##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632708.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99384
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.864153183611045	34.0	31.0	34.0	31.0	34.0
2	33.009035659663525	34.0	33.0	34.0	31.0	34.0
3	33.09395878612251	34.0	33.0	34.0	31.0	34.0
4	36.44800973999839	37.0	37.0	37.0	35.0	37.0
5	36.39139096836513	37.0	37.0	37.0	35.0	37.0
6	36.42830837961845	37.0	37.0	37.0	35.0	37.0
7	36.44277750945826	37.0	37.0	37.0	35.0	37.0
8	36.40717821782178	37.0	37.0	37.0	35.0	37.0
9	38.16576108830395	39.0	39.0	39.0	37.0	39.0
10	38.182091684778236	39.0	38.0	39.0	37.0	39.0
11	38.24928559929164	39.0	39.0	39.0	37.0	39.0
12	38.200505111486756	39.0	38.0	39.0	37.0	39.0
13	38.24398293487885	39.0	39.0	39.0	37.0	39.0
14	39.73290469290831	41.0	40.0	41.0	38.0	41.0
15	39.71775134830556	41.0	40.0	41.0	38.0	41.0
16	39.67741286323754	41.0	40.0	41.0	38.0	41.0
17	39.67525959913065	41.0	40.0	41.0	38.0	41.0
18	39.684858729775414	41.0	40.0	41.0	38.0	41.0
19	39.69070474120583	41.0	40.0	41.0	38.0	41.0
20	39.67087257506238	41.0	40.0	41.0	38.0	41.0
21	39.62611687998068	41.0	40.0	41.0	37.0	41.0
22	39.58244787893423	41.0	39.0	41.0	37.0	41.0
23	39.542461563229494	41.0	39.0	41.0	37.0	41.0
24	39.517014811237225	41.0	39.0	41.0	37.0	41.0
25	39.474341946389764	41.0	39.0	41.0	37.0	41.0
26	39.406031151895675	41.0	39.0	41.0	37.0	41.0
27	39.27829429284392	40.0	39.0	41.0	36.0	41.0
28	39.265284150366256	40.0	39.0	41.0	36.0	41.0
29	39.20770949046124	40.0	39.0	41.0	36.0	41.0
30	39.17284472349674	40.0	39.0	41.0	36.0	41.0
31	39.09422039764952	40.0	39.0	41.0	35.0	41.0
32	39.04992755373098	40.0	39.0	41.0	35.0	41.0
33	38.99383200515174	40.0	39.0	41.0	35.0	41.0
34	38.9519540368671	40.0	39.0	41.0	35.0	41.0
35	38.872313450857284	40.0	38.0	41.0	35.0	41.0
36	38.83482250664091	40.0	38.0	41.0	35.0	41.0
37	38.78110158576833	40.0	38.0	41.0	35.0	41.0
38	38.70624044111728	40.0	38.0	41.0	35.0	41.0
39	38.63904652660388	40.0	38.0	41.0	35.0	41.0
40	38.555582387507044	40.0	38.0	41.0	35.0	41.0
41	38.509689688481046	40.0	38.0	41.0	35.0	41.0
42	38.445685422200754	40.0	38.0	41.0	35.0	41.0
43	37.69056387346052	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	9.0
22	21.0
23	39.0
24	63.0
25	93.0
26	140.0
27	201.0
28	262.0
29	354.0
30	511.0
31	688.0
32	923.0
33	1190.0
34	1901.0
35	2894.0
36	4694.0
37	9038.0
38	25019.0
39	51336.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.86806729453433	16.32959027610078	12.731425581582547	28.07091684778234
2	21.743942686951623	19.325042260323595	31.201199388231505	27.72981566449328
3	21.56383321258955	19.254608387668036	28.28926185301457	30.89229654672784
4	16.025718425501086	15.501489173307576	33.33333333333333	35.139459067858006
5	17.916364807212428	32.56560412138775	32.38046365612171	17.137567415278113
6	34.896965306286724	33.40376720598889	14.847460355791679	16.851807131932706
7	30.673951541495615	26.937937696208646	19.52326330193995	22.86484746035579
8	27.973315624245355	30.271472269178133	19.67922401996297	22.075988086613542
9	27.902881751589792	12.39636158737825	17.150647991628432	42.55010866940353
10	19.93781695242695	22.79038879497706	28.689728728970458	28.582065523625534
11	40.40590034613217	19.007083635192785	18.13772840698704	22.449287611688
12	23.27135152539644	23.12947758190453	26.20341302422925	27.395757868469772
13	36.03497544876439	16.983619093616678	19.974040086935524	27.007365370683413
14	24.43753521693633	19.257626982210414	22.274209128229895	34.03062867262336
15	30.02092892216051	23.470578765193594	19.402519520244706	27.10597279240119
16	26.15410931337036	23.283425903565966	22.27219673186831	28.290268051195362
17	27.973315624245355	22.84572969492071	21.14022377847541	28.04073090235853
18	27.162319890525637	20.74579409160428	22.613297915157368	29.47858810271271
19	29.171697657570633	21.771110037833054	22.27823392095307	26.778958383643243
20	30.284552845528456	20.499275537309828	21.770103839652258	27.44606777750946
21	28.119214360460436	21.472269178137328	21.51956049263463	28.88895596876761
22	28.928197697818565	22.19270707558561	20.683409804395072	28.195685422200757
23	29.19785881027127	21.406866296385736	21.409884890928115	27.985390002414878
24	28.530749416405055	20.734725911615552	21.84456250503099	28.8899621669484
25	28.91914191419142	21.622192707075584	21.971343475810993	27.487321902922
26	28.746075827094902	22.244023182806085	21.567858005312726	27.442042984786287
27	27.411857039362474	21.55980841986638	23.52994445786042	27.498390082910728
28	27.036545117926426	22.82158093858166	21.26801094743621	28.873862996055706
29	27.939104886098363	23.251227561780567	21.55276503260082	27.256902519520242
30	27.457135957498192	21.384729936408277	23.37197134347581	27.786162762617728
31	28.326491185703933	21.892860017709086	22.247041777348468	27.53360701923851
32	25.972993640827497	21.486355952668436	22.84472349673992	29.695926909764147
33	26.40968365129196	21.166384931176044	23.967640666505673	28.45629075102632
34	26.99830958705627	21.093938662158898	23.70401674313773	28.203735007647108
35	26.29296466231989	22.344643000885455	24.021975368268535	27.34041696852612
36	26.672301376479112	20.651211462609677	25.16099170892699	27.515495451984222
37	26.469049343958783	20.141068984947275	25.149923528938263	28.23995814215568
38	25.334057796023508	19.546405860098204	26.442888191258152	28.67664815262014
39	24.704177734846656	18.613660146502458	27.955204056991068	28.726958061659825
40	24.26044433711664	19.491064960154553	29.130443532158097	27.118047170570712
41	22.85377928036706	19.08657329147549	29.789503340577962	28.270144087579492
42	20.986275456813974	19.801980198019802	31.111647750140868	28.10009659502536
43	20.062585526845368	20.51336231184094	30.620623037913546	28.803429123400143
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	1.5
4	1.0
5	1.0
6	3.0
7	5.0
8	4.0
9	3.0
10	2.5
11	2.0
12	2.0
13	13.5
14	25.0
15	46.5
16	68.0
17	59.5
18	51.0
19	51.0
20	63.0
21	75.0
22	72.5
23	70.0
24	90.0
25	110.0
26	110.0
27	127.5
28	145.0
29	210.5
30	276.0
31	361.0
32	446.0
33	446.0
34	611.0
35	776.0
36	906.0
37	1036.0
38	1443.0
39	1850.0
40	1850.0
41	2344.0
42	2838.0
43	3543.5
44	4249.0
45	5537.5
46	6826.0
47	6826.0
48	7677.0
49	8528.0
50	9696.0
51	10864.0
52	11255.0
53	11646.0
54	11646.0
55	10944.5
56	10243.0
57	9765.0
58	9287.0
59	8908.5
60	8530.0
61	8530.0
62	7833.0
63	7136.0
64	5943.0
65	4750.0
66	4061.5
67	3373.0
68	3373.0
69	2860.0
70	2347.0
71	1994.0
72	1641.0
73	1270.0
74	899.0
75	899.0
76	729.5
77	560.0
78	451.5
79	343.0
80	262.5
81	182.0
82	182.0
83	141.0
84	100.0
85	72.0
86	44.0
87	38.5
88	33.0
89	33.0
90	25.5
91	18.0
92	11.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	99384.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.46341463414635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.23841099706301	58.646260967560174
2	5.970872316589458	7.9368912501006195
3	1.9362945468858812	3.860782419705385
4	1.0052381384927482	2.672462368188038
5	0.6131347079662094	2.0375513161072205
6	0.3890756048081872	1.5515575947838687
7	0.2815877918067036	1.3100700313933833
8	0.2286008417355497	1.2154874023987765
9	0.18621128167862658	1.1138613861386137
>10	1.035516395676265	12.884367705063191
>50	0.0817512943954946	3.604201883602994
>100	0.03330608290186817	3.16650567495774
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	290	0.29179747243016985	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	237	0.2384689688481043	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	186	0.18715286162762618	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	174	0.17507848345810192	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	164	0.16501650165016502	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	151	0.15193592529984706	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	149	0.1499235289382597	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	149	0.1499235289382597	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	136	0.1368429525879417	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	131	0.13181196168397327	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	130	0.13080576350317957	TruSeq Adapter, Index 12 (95% over 22bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	120	0.12074378169524269	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	119	0.119737583514449	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	118	0.1187313853336553	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	118	0.1187313853336553	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	118	0.1187313853336553	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	116	0.11671898897206794	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	113	0.11370039442968687	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	111	0.11168799806809948	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	106	0.10665700716413105	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	106	0.10665700716413105	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	105	0.10565080898333737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.001006198180793689	0.0
22	0.0	0.0	0.0	0.001006198180793689	0.0
23	0.0	0.0	0.0	0.002012396361587378	0.0
24	0.0	0.0	0.0	0.0030185945423810675	0.0
25	0.0	0.0	0.0	0.005030990903968446	0.0
26	0.0	0.0	0.0	0.008049585446349513	0.0
27	0.0	0.0	0.0	0.04527891813571601	0.0
28	0.0	0.0	0.0	0.20123963615873783	0.0
29	0.0	0.0	0.0	0.4165660468485873	0.0
30	0.0	0.0	0.0	0.7325122756178056	0.0
31	0.0	0.0	0.0	1.521371649360058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	145	0.0	33.172413	1
CCTTGTA	25	0.005472505	29.6	2
ATACTGT	35	8.812249E-4	26.428572	36
CTGGTTC	50	8.982433E-6	25.900002	4
ACTGGTT	55	1.8800794E-5	23.545454	3
TGGTTCA	40	0.0019188629	23.125	5
GTCCACG	40	0.0019188629	23.125	19
GGATTAG	40	0.0019188629	23.125	1
GTATCAA	210	0.0	22.90476	2
TACTGTC	65	2.6421221E-6	22.76923	37
TACTGGT	65	2.6421221E-6	22.76923	2
TTGTGTA	50	2.6781025E-4	22.2	21
TTCTTCT	85	5.0617018E-8	21.764706	15
GTACTGG	70	5.02496E-6	21.142859	1
TATACAC	80	6.8293957E-7	20.8125	36
ATACACA	80	6.8293957E-7	20.8125	37
AGCCGGT	90	9.2615664E-8	20.555557	6
TTAGCCG	90	9.2615664E-8	20.555557	4
TAGCCGG	90	9.2615664E-8	20.555557	5
TCCAGGA	45	0.0038016252	20.555557	2
>>END_MODULE