ERR1632707.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632707.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78952
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	268	0.3394467524571892	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	162	0.20518796230621136	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	146	0.1849224845475732	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	140	0.1773229303880839	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	131	0.16592359914884994	RNA PCR Primer, Index 17 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	127	0.1608572297091904	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	126	0.15959063734927553	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	123	0.15579086026953085	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	110	0.13932515959063735	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	109	0.13805856723072246	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	108	0.13679197487080758	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	103	0.13045901307123317	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	102	0.12919242071131828	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	100	0.1266592359914885	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	95	0.12032627419191408	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	95	0.12032627419191408	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	95	0.12032627419191408	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	91	0.11525990475225453	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	90	0.11399331239233966	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	89	0.11272672003242476	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	87	0.110193535312595	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	87	0.110193535312595	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	87	0.110193535312595	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	83	0.10512716587293545	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	82	0.10386057351302058	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGCAA	25	0.0054657757	29.6	10
GCGGGTA	40	5.8714108E-5	27.75	22
CGGGTAA	45	1.3080274E-4	24.666666	23
TTCTGCG	45	1.3080274E-4	24.666666	18
GTAACGT	45	1.3080274E-4	24.666666	26
GGTATCA	115	0.0	24.130436	1
AACGTCA	40	0.0019153873	23.125	28
ACGTCAA	40	0.0019153873	23.125	29
CGTGCCA	140	0.0	22.464285	10
GTGCCAG	140	0.0	22.464285	11
TCTGCGG	50	2.6713443E-4	22.2	19
GGTAACG	50	2.6713443E-4	22.2	25
TAACGTC	50	2.6713443E-4	22.2	27
CTGCGGG	50	2.6713443E-4	22.2	20
GCGGTAA	135	0.0	21.925924	23
CCGCGGT	130	1.8189894E-12	21.346153	21
GGTAATA	140	0.0	21.142857	25
CTAACTC	140	0.0	21.142857	3
GCTAACT	140	0.0	21.142857	2
TAACTCC	140	0.0	21.142857	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content