FastQCFastQC Report
Fri 10 Feb 2017
ERR1632706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199519
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10320.5172439717520637No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8550.42853061613179694No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6840.3428244929054376No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6050.3032292663856575No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5720.28668948821916707No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4490.2250412241440665No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4490.2250412241440665No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4470.22403881334609735No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4470.22403881334609735No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4330.21702193776031356No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4220.21150867837148343No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4210.2110074729724989No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4150.20800024057859148No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4110.20599541898265328No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4100.2054942135836687No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4060.20348939198773047No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4030.2019857757907768No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4010.2009833649928077No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3840.19246287321007022No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3830.19196166781108567No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3750.1879520246192092No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3710.18594720302327095No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3700.1854459976242864No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3700.1854459976242864No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3650.18293997062936362No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3620.18143635443240996No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3570.17893032743748716No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3520.17642430044256435No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3410.17091104105373425No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3250.16289175466998132No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3220.16138813847302763No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3200.16038572767505851No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3150.15787970068013574No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3110.1558748790841975No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3060.1533688520892747No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3000.15036161969536735No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2970.1488580034984137No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2930.14685318190247545No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2930.14685318190247545No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2810.14083871711466076No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2760.13833269011973798No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2740.13733027932176883No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2610.13081460913496962No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2580.12931099293801593No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2550.12780737674106224No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2500.12530134974613946No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2500.12530134974613946No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2480.12429893894817035No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2410.12079050115527844No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2400.12028929575629388No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2380.11928688495832476No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2360.11828447416035565No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2340.11728206336238654No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2310.11577844716543287No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2300.11527724176644831No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2300.11527724176644831No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2260.11327242017051009No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2230.11176880397355639No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT2180.10926277697863361TruSeq Adapter, Index 11 (95% over 23bp)
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2180.10926277697863361No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2130.10675674998371083No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2120.10625554458472627No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2070.10374951758980347No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2060.10324831219081891No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA2060.10324831219081891No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2050.10274710679183435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCT200.001838292637.04
TCCCTAG200.001838292637.02
ATCCCTA200.001838292637.01
CTAGCTG200.001838292637.05
GGTATCA5300.031.0660381
ATACGGC551.839544E-830.27272829
CCCTAGC250.00548559629.63
ACTCCGA250.00548559629.68
ATACCGC250.00548559629.66
GAAATTC405.915928E-527.7514
TAGCGAA405.915928E-527.7510
TTGTCGG405.915928E-527.7522
ATTCCTT405.915928E-527.7517
AAATTCC405.915928E-527.7515
TAAGGTA405.915928E-527.755
TTCCTTG405.915928E-527.7518
TTGCTCC358.843619E-426.4285713
ATACACA601.3281715E-624.66666837
CGAAATT451.3178645E-424.66666613
GCGAAAT451.3178645E-424.66666612
Produced by FastQC (version 0.11.5)