FastQCFastQC Report
Fri 10 Feb 2017
ERR1632705.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632705.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35786
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1830.5113731626893199No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1260.35209299726149895No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1090.3045883865198681No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1040.2906164421840943No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1000.27943888671547534No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA970.2710557201140111No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC890.24870060917677306No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA880.24590622030961828No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT840.2347286648409993No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA820.22913988710668975No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT800.22355110937238026No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC780.21796233163807074No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT780.21796233163807074TruSeq Adapter, Index 11 (95% over 21bp)
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA780.21796233163807074No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG730.203990387302297No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC720.20119599843514221No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT690.19281283183367798No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA680.19001844296652323No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC670.18722405409936846No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT650.18163527636505897No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC620.1732521097635947No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC620.1732521097635947No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT610.17045772089643996No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA590.16486894316213044No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT580.1620745542949757No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA560.15648577656066617No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC560.15648577656066617No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA560.15648577656066617No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA550.15369138769351143No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT550.15369138769351143No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC540.15089699882635665No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC540.15089699882635665No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA530.1481026099592019No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC530.1481026099592019No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA530.1481026099592019No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG520.14530822109204716No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC500.13971944335773767No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG490.1369250544905829No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG480.13413066562342815No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA470.1313362767562734No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC460.12854188788911866No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT460.12854188788911866No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA460.12854188788911866No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG440.12295311015480914No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT440.12295311015480914No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA430.1201587212876544No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC430.1201587212876544No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC420.11736433242049965No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG420.11736433242049965No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC410.11456994355334488No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC410.11456994355334488No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT400.11177555468619013No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG400.11177555468619013No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA400.11177555468619013No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG400.11177555468619013No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT380.10618677695188063No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA380.10618677695188063No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT380.10618677695188063No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA370.10339238808472588No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA370.10339238808472588No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC370.10339238808472588No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT360.10059799921757111No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT360.10059799921757111No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC360.10059799921757111No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC360.10059799921757111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCCG200.001818187137.020
ACGGGCT200.001818187137.017
GCTCCGT200.001818187137.021
GGGCTCC250.005426283529.619
GGTATCA1050.028.1904751
AATGAGC405.7831545E-527.75000234
GTACTGG508.773815E-625.9000021
TACTGGT508.773815E-625.9000022
ACTGGTT508.773815E-625.9000023
CAATGAG451.2885239E-424.66666633
TCAATGA451.2885239E-424.66666632
GAGCAAA451.2885239E-424.66666637
GCTTCTT451.2885239E-424.66666613
TGAGCAA451.2885239E-424.66666636
ATGAGCA451.2885239E-424.66666635
TGGTTCA551.8367182E-523.5454545
CTGGTTC551.8367182E-523.5454544
TCTTATA1101.8189894E-1223.54545437
CTTATAC400.001895024723.12500237
AACGTCA502.631833E-422.228
Produced by FastQC (version 0.11.5)