FastQCFastQC Report
Fri 10 Feb 2017
ERR1632704.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632704.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224523
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13510.6017200910374438No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10630.47344815453205236No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8710.3879335301951248No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7530.335377667321388No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6640.29573807583187467No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6510.2899480231423952No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6480.2886118571371307No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6470.2881664684687092No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5980.2663424237160558No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5830.25966159368973335No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5740.25565309567393985No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5670.25253537499498935No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5540.2467453223055099No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5360.23872832627392293No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5330.23739216026865845No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC5270.23471982825812948No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5260.23427443958970798No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5140.22892977556865No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5080.226257443558121No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4700.20933267415810408No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4680.20844189682126105No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4590.2044333988054676No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4550.20265184413178158No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4390.1955256254370376No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4090.18216396538439267No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4060.18082779937912818No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4050.1803824107107067No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4050.1803824107107067No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4020.1790462447054422No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3880.17281080334754123No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3860.17192002601069822No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3790.16880230533174775No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3750.16702075065806177No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3700.16479380731595428No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3680.16390302997911127No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3650.1625668639738468No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3620.1612306979685823No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3610.1607853093001608No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3570.15900375462647479No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3560.1585583659580533No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3440.15321370193699532No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3390.15098675859488783No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3360.14965059258962332No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3270.14564209457382987No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3250.14475131723698684No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3190.14207898522645787No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3110.13851587587908587No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3090.1376250985422429No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3030.1349527665317139No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3000.1336166005264494No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3000.1336166005264494No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2980.1327258231896064No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2970.1322804345211849No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2960.1318350458527634No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2930.13049887984749892No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2900.1291627138422344No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2860.12738115916854845No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2840.12649038183170544No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2800.12470882715801945No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2790.12426343848959795No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2740.12203649514749046No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2660.11847338580011847No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2660.11847338580011847No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2600.11580105378958948No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2570.114464887784325No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2550.11357411044748199No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2530.112683333110639No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT2520.1122379444422175No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2480.1104563897685315No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2450.10912022376326702No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT2440.10867483509484552No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2440.10867483509484552No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2390.10644789175273803No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2380.10600250308431652No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2360.10511172574747354No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG2330.10377555974220903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTTG200.001838783437.05
GTTGTTT200.001838783437.08
GGTTGTT200.001838783437.07
CAATGCT403.8098733E-837.033
TTGTTTC200.001838783437.09
TCAATGC451.07338565E-732.8888932
TCCCTCT250.00548704329.614
TCTGGGT250.00548704329.63
GGTATCA4100.029.3292681
CTCTATG856.91216E-1126.1176451
TCTATGG951.0913936E-1125.315792
ATACGGC751.3611498E-824.66666629
ATACACA551.8938323E-523.54545437
TACGGCT802.7011993E-823.12530
AGTTCCG400.001926376623.12532
AACACCT603.7091202E-521.58333436
TAACACC705.0718118E-621.14285735
AACGTCA2700.020.55555528
GCCGTCT450.003816368820.55555536
CTATGGG1101.7334969E-920.1818183
Produced by FastQC (version 0.11.5)