FastQCFastQC Report
Fri 10 Feb 2017
ERR1632703.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632703.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145555
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3050.2095427845144447No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2570.1765655594105321No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2540.17450448284153758No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2410.16557315104256123No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT2370.1628250489505685No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2270.15595479372058674No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT2220.1525196661055958No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2000.13740510459963587No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1940.1332829514616468No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1860.12778674727766137No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1790.12297756861667411No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1780.12229054309367592No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1730.11885541547868503No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1710.11748136443268868No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1590.10923705815671053No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1570.10786300711071416No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1550.10648895606471781No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1500.10305382844972692No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1470.10099275188073238No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1460.10030572635773419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCA251.2283358E-436.9999961
GGTATCA1400.033.0357171
GTGCATG303.5841882E-430.8333348
CTCGTAA303.5841882E-430.83333431
GGTGCAT303.5841882E-430.8333347
CGTCCGC303.5841882E-430.83333410
GCGCGGG250.00548078129.59999813
ACTCGTC250.00548078129.59999818
ATACACA250.00548078129.59999837
CAAAGAA250.00548078129.59999827
CGGTTGG358.8320737E-426.42857223
AAGGTGC358.8320737E-426.4285725
CCGTCCG358.8320737E-426.4285729
TGCATGC358.8320737E-426.4285729
AGGGGCG358.8320737E-426.42857218
TCACCCG659.274481E-825.6153855
TATACAC659.274481E-825.61538537
GCGGTTG400.001923138423.12500222
TAACCCT400.001923138423.12500235
CGAGGGG400.001923138423.12500216
Produced by FastQC (version 0.11.5)