##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632703.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145555
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89384768644155	34.0	31.0	34.0	31.0	34.0
2	33.04326886743843	34.0	33.0	34.0	31.0	34.0
3	33.11471952183024	34.0	33.0	34.0	31.0	34.0
4	36.45887808732095	37.0	37.0	37.0	35.0	37.0
5	36.40273436158153	37.0	37.0	37.0	35.0	37.0
6	36.43969633471883	37.0	37.0	37.0	35.0	37.0
7	36.44662155199066	37.0	37.0	37.0	35.0	37.0
8	36.41121912679056	37.0	37.0	37.0	35.0	37.0
9	38.17780907560716	39.0	39.0	39.0	37.0	39.0
10	38.192112946995984	39.0	38.0	39.0	37.0	39.0
11	38.277833121500464	39.0	39.0	39.0	37.0	39.0
12	38.2229603929786	39.0	39.0	39.0	37.0	39.0
13	38.26001854968912	39.0	39.0	39.0	37.0	39.0
14	39.755693724021846	41.0	40.0	41.0	38.0	41.0
15	39.75941053210127	41.0	40.0	41.0	38.0	41.0
16	39.72387070179657	41.0	40.0	41.0	38.0	41.0
17	39.713001958022744	41.0	40.0	41.0	38.0	41.0
18	39.69390951873862	41.0	40.0	41.0	38.0	41.0
19	39.688083542303595	41.0	40.0	41.0	38.0	41.0
20	39.66942392909897	41.0	40.0	41.0	37.0	41.0
21	39.64103603448868	41.0	40.0	41.0	37.0	41.0
22	39.59987633540586	41.0	40.0	41.0	37.0	41.0
23	39.56485864449864	41.0	40.0	41.0	37.0	41.0
24	39.541513517227166	41.0	39.0	41.0	37.0	41.0
25	39.48901102675964	41.0	39.0	41.0	37.0	41.0
26	39.38833430661949	41.0	39.0	41.0	37.0	41.0
27	39.296245405516814	40.0	39.0	41.0	36.0	41.0
28	39.2614338222665	40.0	39.0	41.0	36.0	41.0
29	39.220741300539316	40.0	39.0	41.0	36.0	41.0
30	39.15079523204287	40.0	39.0	41.0	36.0	41.0
31	39.10659200989317	40.0	39.0	41.0	35.0	41.0
32	39.047452852873484	40.0	39.0	41.0	35.0	41.0
33	38.98068084229329	40.0	39.0	41.0	35.0	41.0
34	38.93062416268764	40.0	39.0	41.0	35.0	41.0
35	38.835498608773314	40.0	38.0	41.0	35.0	41.0
36	38.7942702071382	40.0	38.0	41.0	35.0	41.0
37	38.73555700594277	40.0	38.0	41.0	35.0	41.0
38	38.66515063034592	40.0	38.0	41.0	35.0	41.0
39	38.56661742983752	40.0	38.0	41.0	35.0	41.0
40	38.450455154408985	40.0	38.0	41.0	35.0	41.0
41	38.40092748445605	40.0	38.0	41.0	35.0	41.0
42	38.34323108103466	40.0	38.0	41.0	34.0	41.0
43	37.56498917934801	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	2.0
20	9.0
21	19.0
22	33.0
23	62.0
24	103.0
25	136.0
26	210.0
27	300.0
28	426.0
29	510.0
30	735.0
31	981.0
32	1354.0
33	1814.0
34	2708.0
35	4583.0
36	7028.0
37	13023.0
38	35612.0
39	75904.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.78894575933496	15.542578406787813	12.2263062072756	28.442169626601626
2	21.823365738037168	18.118924118030986	31.74744941774587	28.310260726185977
3	22.122908866064375	18.73999519082134	27.816976400673283	31.320119542441
4	16.710521795884716	15.207309951564701	31.737831060423893	36.34433719212669
5	18.22197794648071	31.972793789289273	31.718594345779945	18.086633918450072
6	36.81288859881144	31.348287588883927	14.855552883789633	16.983270928514994
7	31.798976331970735	26.168802171000653	19.061523135584487	22.97069836144413
8	28.361100614887842	29.84576277008691	19.56236474185016	22.23077187317509
9	28.318505032461953	12.621345882999554	16.89052248291024	42.16962660162825
10	20.401909930953934	22.564666277352206	28.793239668853698	28.240184122840162
11	40.24458108618735	18.560681529318813	18.219916869911717	22.974820514582117
12	23.993679365188417	22.42932224932156	25.685136202809932	27.89186218268009
13	35.421661914740135	16.58891827831404	20.249390264848337	27.74002954209749
14	24.59963587647281	19.00724811926763	22.337260829239806	34.05585517501975
15	30.53210126756209	22.692453024629867	19.314348528047816	27.46109717976023
16	26.921782144206656	22.73573563257875	21.185806052694858	29.156676170519734
17	27.68575452578063	23.07169111332486	21.23046271168974	28.012091649204766
18	27.2824705437807	20.406719109614922	22.66703308027893	29.643777266325444
19	28.734155473875855	21.633059668166673	21.95939679159081	27.673388066366666
20	29.658204802308408	20.436261207103847	22.100237023805434	27.80529696678232
21	29.41293669059806	20.82649170416681	20.983133523410395	28.77743808182474
22	29.587441173439593	21.98138160832675	20.36961973137302	28.06155748686064
23	29.457593349592937	21.3960358627323	20.924049328432552	28.22232145924221
24	29.016522963828105	20.910308817972588	21.405654220054274	28.667513998145033
25	29.061179622822987	21.132905087423996	21.930541719624884	27.875373570128133
26	29.467211706914913	21.348631101645427	21.644052076534642	27.540105114905018
27	28.563773144172306	20.870461337638694	22.954209748892172	27.61155576929683
28	27.201401532066917	22.098862972759438	21.576036549757823	29.12369894541582
29	28.59743739479922	22.140771529662327	21.551990656452887	27.709800419085568
30	27.851327676823196	20.97008003847343	22.98787399951908	28.190718285184296
31	28.424993988526676	21.2826766514376	21.813747380715192	28.478581979320534
32	26.64903301157638	21.58084572841881	22.148328810415308	29.621792449589503
33	27.298959156332657	21.25313455394868	23.41863900243894	28.029267287279723
34	27.36628765758648	21.382982377795333	22.7542853216997	28.496444642918483
35	27.268043007797736	22.288482017106936	23.582838102435506	26.86063687265982
36	27.560715880594966	20.789392325924908	23.349936450139122	28.299955343341004
37	26.807735907388956	20.965957885335442	23.673525471471265	28.552780735804333
38	26.832468826216893	20.03435127614991	24.77757548692934	28.355604410703855
39	25.812235924564597	19.236714643949025	26.308955377692282	28.642094053794096
40	25.72017450448284	20.039160454810897	27.33949366218955	26.901171378516715
41	24.035587922091306	19.49022706193535	27.881556799835117	28.592628216138227
42	22.43275737693655	20.152519666105594	28.78499536257772	28.62972759438013
43	21.964205970251797	20.146336436398613	29.061179622822987	28.828277970526607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	3.5
4	5.0
5	5.0
6	9.0
7	13.0
8	12.0
9	11.0
10	15.0
11	19.0
12	19.0
13	44.0
14	69.0
15	109.5
16	150.0
17	140.0
18	130.0
19	130.0
20	159.0
21	188.0
22	161.5
23	135.0
24	136.5
25	138.0
26	138.0
27	201.5
28	265.0
29	343.5
30	422.0
31	567.5
32	713.0
33	713.0
34	932.0
35	1151.0
36	1353.5
37	1556.0
38	2008.5
39	2461.0
40	2461.0
41	3035.0
42	3609.0
43	4456.0
44	5303.0
45	7153.0
46	9003.0
47	9003.0
48	10219.0
49	11435.0
50	12931.0
51	14427.0
52	15445.0
53	16463.0
54	16463.0
55	15751.5
56	15040.0
57	14317.5
58	13595.0
59	12897.5
60	12200.0
61	12200.0
62	11755.0
63	11310.0
64	9263.0
65	7216.0
66	6274.5
67	5333.0
68	5333.0
69	4608.0
70	3883.0
71	3411.5
72	2940.0
73	2790.5
74	2641.0
75	2641.0
76	2498.5
77	2356.0
78	1569.5
79	783.0
80	537.5
81	292.0
82	292.0
83	227.5
84	163.0
85	117.5
86	72.0
87	55.0
88	38.0
89	38.0
90	25.0
91	12.0
92	9.5
93	7.0
94	5.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	145555.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.591597677853734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.94933339587023	50.945003606884
2	6.207567745037112	7.274226237504723
3	2.0684075372583046	3.635739067706365
4	1.0834515671353024	2.539246333001271
5	0.7164382116013742	2.098862972759438
6	0.4725443523328213	1.6612277146095977
7	0.36115052237843415	1.4812270275840747
8	0.2696903251527268	1.26412696231665
9	0.22747792643316955	1.1995465631548212
>10	1.4317038565716498	16.567620487101095
>50	0.15126109541174676	6.070557521211914
>100	0.06097346481713824	5.262615506166054
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	305	0.2095427845144447	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	257	0.1765655594105321	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	254	0.17450448284153758	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	241	0.16557315104256123	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	237	0.1628250489505685	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	227	0.15595479372058674	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	222	0.1525196661055958	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	200	0.13740510459963587	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	194	0.1332829514616468	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	186	0.12778674727766137	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	179	0.12297756861667411	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	178	0.12229054309367592	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	173	0.11885541547868503	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	171	0.11748136443268868	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	159	0.10923705815671053	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	157	0.10786300711071416	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	155	0.10648895606471781	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	150	0.10305382844972692	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	147	0.10099275188073238	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	146	0.10030572635773419	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	6.870255229981793E-4	0.0
17	6.870255229981793E-4	0.0	0.0	6.870255229981793E-4	0.0
18	6.870255229981793E-4	0.0	0.0	6.870255229981793E-4	0.0
19	6.870255229981793E-4	0.0	0.0	6.870255229981793E-4	0.0
20	6.870255229981793E-4	0.0	0.0	0.0020610765689945383	0.0
21	0.0013740510459963587	0.0	0.0	0.003435127614990897	0.0
22	0.0013740510459963587	0.0	0.0	0.005496204183985435	0.0
23	0.0013740510459963587	0.0	0.0	0.005496204183985435	0.0
24	0.0013740510459963587	0.0	0.0	0.008244306275978153	0.0
25	0.0013740510459963587	0.0	0.0	0.00961835732197451	0.0
26	0.0013740510459963587	0.0	0.0	0.01099240836797087	0.0
27	0.0013740510459963587	0.0	0.0	0.030229123011919892	0.0
28	0.0013740510459963587	0.0	0.0	0.13534402803064133	0.0
29	0.0013740510459963587	0.0	0.0	0.2899247707052317	0.0
30	0.0013740510459963587	0.0	0.0	0.49397135103569095	0.0
31	0.0013740510459963587	0.0	0.0	1.022981003744289	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTCA	25	1.2283358E-4	36.999996	1
GGTATCA	140	0.0	33.035717	1
GTGCATG	30	3.5841882E-4	30.833334	8
CTCGTAA	30	3.5841882E-4	30.833334	31
GGTGCAT	30	3.5841882E-4	30.833334	7
CGTCCGC	30	3.5841882E-4	30.833334	10
GCGCGGG	25	0.005480781	29.599998	13
ACTCGTC	25	0.005480781	29.599998	18
ATACACA	25	0.005480781	29.599998	37
CAAAGAA	25	0.005480781	29.599998	27
CGGTTGG	35	8.8320737E-4	26.428572	23
AAGGTGC	35	8.8320737E-4	26.428572	5
CCGTCCG	35	8.8320737E-4	26.428572	9
TGCATGC	35	8.8320737E-4	26.428572	9
AGGGGCG	35	8.8320737E-4	26.428572	18
TCACCCG	65	9.274481E-8	25.615385	5
TATACAC	65	9.274481E-8	25.615385	37
GCGGTTG	40	0.0019231384	23.125002	22
TAACCCT	40	0.0019231384	23.125002	35
CGAGGGG	40	0.0019231384	23.125002	16
>>END_MODULE