FastQCFastQC Report
Fri 10 Feb 2017
ERR1632702.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632702.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15218
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT400.2628466289919832RNA PCR Primer, Index 29 (95% over 23bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA350.22999080036798528No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA330.2168484689183861No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA300.1971349717439874No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA220.14456564594559074No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC220.14456564594559074No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT210.13799448022079117No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT200.1314233144959916No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC190.12485214877119202RNA PCR Primer, Index 29 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA190.12485214877119202No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA180.11828098304639244No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC170.11170981732159285No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA170.11170981732159285No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC160.10513865159679327No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA160.10513865159679327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACCA250.005329518629.67
TCTCTTA700.002438211615.85714337
GTCTCTT1151.9405865E-514.47826137
TGTCTCT1553.1768545E-410.74193636
CTGTCTC1800.00125901529.2535
Produced by FastQC (version 0.11.5)