##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632702.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15218
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.001905638060194	34.0	33.0	34.0	31.0	34.0
2	33.151662504928375	34.0	33.0	34.0	31.0	34.0
3	33.20554606387173	34.0	33.0	34.0	31.0	34.0
4	36.50729399395453	37.0	37.0	37.0	35.0	37.0
5	36.47811801813642	37.0	37.0	37.0	35.0	37.0
6	36.53443290839795	37.0	37.0	37.0	35.0	37.0
7	36.51511368116704	37.0	37.0	37.0	35.0	37.0
8	36.510973846760415	37.0	37.0	37.0	35.0	37.0
9	38.29773951899067	39.0	39.0	39.0	37.0	39.0
10	38.30194506505454	39.0	39.0	39.0	37.0	39.0
11	38.36430542778289	39.0	39.0	39.0	37.0	39.0
12	38.34156919437508	39.0	39.0	39.0	37.0	39.0
13	38.3803390721514	39.0	39.0	39.0	37.0	39.0
14	39.93087133657511	41.0	40.0	41.0	38.0	41.0
15	39.9214088579314	41.0	40.0	41.0	38.0	41.0
16	39.88382178998555	41.0	40.0	41.0	38.0	41.0
17	39.87238796162439	41.0	40.0	41.0	38.0	41.0
18	39.87915626232093	41.0	40.0	41.0	38.0	41.0
19	39.90340386384545	41.0	40.0	41.0	38.0	41.0
20	39.876790642660005	41.0	40.0	41.0	38.0	41.0
21	39.86607964252858	41.0	40.0	41.0	38.0	41.0
22	39.82829543961099	41.0	40.0	41.0	38.0	41.0
23	39.793533972926795	41.0	40.0	41.0	38.0	41.0
24	39.741424628729135	41.0	40.0	41.0	38.0	41.0
25	39.73025364699698	41.0	40.0	41.0	38.0	41.0
26	39.63950584833749	41.0	40.0	41.0	37.0	41.0
27	39.58391378630569	41.0	40.0	41.0	37.0	41.0
28	39.543698252069916	41.0	40.0	41.0	37.0	41.0
29	39.5262846628992	41.0	40.0	41.0	37.0	41.0
30	39.45189906689447	41.0	39.0	41.0	37.0	41.0
31	39.381193323695626	41.0	39.0	41.0	36.0	41.0
32	39.30293073991326	41.0	39.0	41.0	36.0	41.0
33	39.237613352608754	41.0	39.0	41.0	36.0	41.0
34	39.231370745170196	41.0	39.0	41.0	36.0	41.0
35	39.142462872913654	41.0	39.0	41.0	35.0	41.0
36	39.078459718754104	41.0	39.0	41.0	35.0	41.0
37	39.02687606781443	41.0	39.0	41.0	35.0	41.0
38	38.92495728742279	40.0	39.0	41.0	35.0	41.0
39	38.880799053752135	40.0	39.0	41.0	35.0	41.0
40	38.774280457353136	40.0	38.0	41.0	35.0	41.0
41	38.77060060454725	40.0	38.0	41.0	35.0	41.0
42	38.68885530293074	40.0	38.0	41.0	35.0	41.0
43	38.00781968721251	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	2.0
23	5.0
24	9.0
25	9.0
26	20.0
27	22.0
28	40.0
29	46.0
30	100.0
31	80.0
32	121.0
33	166.0
34	235.0
35	378.0
36	575.0
37	1150.0
38	2914.0
39	9342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66657905112367	16.631620449467736	13.12261795242476	27.579182546983834
2	20.810881850440268	19.733210671573136	32.07385990274675	27.38204757523985
3	20.90944933631226	20.015770797739517	29.018267840714945	30.05651202523328
4	16.145354185832566	15.330529635957419	32.63240898935471	35.89170718885531
5	17.223025364699698	33.06610592719149	32.61269549218031	17.098173215928504
6	36.22026547509528	32.776974635300306	14.84426337232225	16.158496517282167
7	30.877907740833223	27.158627940596663	19.437508213957155	22.52595610461296
8	28.183729793665396	30.884478906558023	19.99605730056512	20.935733999211458
9	28.196872125114997	12.984623472203968	16.97989223288211	41.83861216979892
10	19.71349717439874	24.22131686161125	29.649099750295704	26.416086213694314
11	38.809304770666316	19.483506374030753	18.234984886318834	23.472203968984097
12	23.26192666579051	23.29478249441451	26.4817978709423	26.961492968852674
13	35.50400841109212	17.019319227230913	20.91602050203706	26.5606518596399
14	24.52359048495203	19.910632146142724	22.263109475620976	33.30266789328427
15	29.182546983834932	24.66815613089762	19.50321987120515	26.646077014062296
16	25.956104612958335	23.774477592324878	21.816270206334604	28.453147588382176
17	27.257195426468655	23.72847943225128	21.507425417269026	27.506899724011042
18	27.00091996320147	21.238007622552242	22.880799053752135	28.88027336049415
19	28.380864765409385	22.05940333815219	22.769089236430542	26.790642660007887
20	29.7082402418189	20.876593507688266	22.68366408200815	26.73150216848469
21	26.84321198580628	22.177684321198583	22.02654750952819	28.95255618346695
22	28.748850045998157	22.289394138520173	21.159153633854647	27.802602181627023
23	28.499145748455778	22.131686161124982	22.065974503876987	27.30319358654225
24	28.269154948087795	21.8491260349586	22.407675121566566	27.47404389538704
25	28.374293599684584	21.8819818635826	22.295965304244973	27.447759232487844
26	27.907740833223816	22.926797213825733	21.8425548692338	27.32290708371665
27	27.907740833223816	21.592850571691418	23.656196609278485	26.84321198580628
28	26.337232224996715	22.440530950190563	22.322249967144174	28.89998685766855
29	27.39518990668945	23.610198449204887	21.34314627414903	27.65146536995663
30	26.869496648705482	22.48652911026416	23.3999211460113	27.244053095019055
31	28.702851885924563	21.678275726113814	22.309107635694573	27.30976475226705
32	26.600078853988695	21.369430937048232	22.95308187672493	29.077408332238143
33	26.370088053620712	21.770272046261006	23.73505059797608	28.1245893021422
34	26.711788671310288	21.586279405966618	23.353922985937707	28.348008936785384
35	26.626363516887896	22.61795242476015	24.635300302273624	26.12038375607833
36	26.416086213694314	21.901695360757	24.839006439742413	26.84321198580628
37	27.48061506111184	21.244578788277042	23.656196609278485	27.618609541332635
38	26.21238007622552	20.2851885924563	25.962675778683135	27.539755552635036
39	25.29898804047838	19.36522539098436	26.639505848337496	28.696280720199763
40	24.35931134183204	21.605992903141015	27.769746353003022	26.26494940202392
41	23.071362859771323	19.56893152845315	28.834275200420556	28.525430411354975
42	22.447102115915364	20.653173873045077	29.189118149559732	27.710605861479827
43	21.27086345117624	21.284005782625837	29.189118149559732	28.256012616638195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	1.5
11	2.0
12	2.0
13	5.0
14	8.0
15	11.5
16	15.0
17	12.0
18	9.0
19	9.0
20	13.0
21	17.0
22	16.0
23	15.0
24	29.0
25	43.0
26	43.0
27	39.5
28	36.0
29	65.5
30	95.0
31	107.5
32	120.0
33	120.0
34	139.5
35	159.0
36	198.0
37	237.0
38	311.5
39	386.0
40	386.0
41	436.0
42	486.0
43	575.5
44	665.0
45	832.0
46	999.0
47	999.0
48	1155.5
49	1312.0
50	1436.5
51	1561.0
52	1669.0
53	1777.0
54	1777.0
55	1659.0
56	1541.0
57	1424.0
58	1307.0
59	1217.0
60	1127.0
61	1127.0
62	1083.0
63	1039.0
64	856.0
65	673.0
66	617.5
67	562.0
68	562.0
69	448.0
70	334.0
71	308.5
72	283.0
73	222.0
74	161.0
75	161.0
76	135.0
77	109.0
78	85.5
79	62.0
80	49.5
81	37.0
82	37.0
83	29.0
84	21.0
85	16.0
86	11.0
87	6.0
88	1.0
89	1.0
90	2.0
91	3.0
92	2.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	15218.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.09593901958208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.05660841742554	72.13168616112499
2	5.2916564115185825	8.476803784991457
3	1.9853966691279021	4.770666316204495
4	0.9680859791615392	3.1015902221054015
5	0.49224710804824023	1.9713497174398737
6	0.28714414636147345	1.3799448022079117
7	0.23791943555664946	1.3339466421343145
8	0.12306177701206006	0.7885398869759496
9	0.1066535400771187	0.7688263898015508
>10	0.4512265157108869	5.276646077014062
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	40	0.2628466289919832	RNA PCR Primer, Index 29 (95% over 23bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	35	0.22999080036798528	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	33	0.2168484689183861	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	30	0.1971349717439874	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	22	0.14456564594559074	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	22	0.14456564594559074	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	21	0.13799448022079117	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	20	0.1314233144959916	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	19	0.12485214877119202	RNA PCR Primer, Index 29 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	19	0.12485214877119202	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	18	0.11828098304639244	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	17	0.11170981732159285	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	17	0.11170981732159285	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	16	0.10513865159679327	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	16	0.10513865159679327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.013142331449599158	0.0
28	0.0	0.0	0.0	0.07228282297279537	0.0
29	0.0	0.0	0.0	0.2628466289919832	0.0
30	0.0	0.0	0.0	0.4139834406623735	0.0
31	0.0	0.0	0.0	0.9396766986463398	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGACCA	25	0.0053295186	29.6	7
TCTCTTA	70	0.0024382116	15.857143	37
GTCTCTT	115	1.9405865E-5	14.478261	37
TGTCTCT	155	3.1768545E-4	10.741936	36
CTGTCTC	180	0.0012590152	9.25	35
>>END_MODULE