FastQCFastQC Report
Fri 10 Feb 2017
ERR1632701.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632701.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26563
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA620.23340737115536647No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA530.199525655987652No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT530.199525655987652TruSeq Adapter, Index 8 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA500.18823175093174718No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT450.16940857583857247No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA370.1392914956894929No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT310.11670368557768324No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT280.1054097805217784No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA280.1054097805217784No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT270.10164514550314346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAT200.001809744337.030
GTCAATG200.001809744337.031
AACGTCA303.5064598E-430.83333428
ACGTCAA250.00540137229.629
GGGTAAC250.00540137229.624
CGGGTAA250.00540137229.623
TCTGCGG250.00540137229.619
TGCGGGT250.00540137229.621
GCGGGTA250.00540137229.622
TCTTATA500.006859826418.536
ACCTGTC700.002504186515.85714337
TATCAAC700.002504186515.8571431
ATCAACG700.002504186515.8571432
CTCTTAT855.123325E-415.23529337
AACGCAG750.003965734514.85
ACGCAGA750.003965734514.86
TCAACGC750.003965734514.83
CGCAGAG750.003965734514.87
CAACGCA750.003965734514.84
CAGAGTA800.00608755413.8759