##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632701.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26563
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.029778262997404	33.0	31.0	34.0	30.0	34.0
2	32.23672024997177	33.0	31.0	34.0	31.0	34.0
3	32.32985732033279	34.0	31.0	34.0	31.0	34.0
4	36.00176937845876	37.0	35.0	37.0	35.0	37.0
5	35.570982193276365	37.0	35.0	37.0	33.0	37.0
6	35.48401912434589	37.0	35.0	37.0	33.0	37.0
7	35.50355758009261	37.0	35.0	37.0	33.0	37.0
8	35.42841546512066	37.0	35.0	37.0	33.0	37.0
9	37.00918570944547	39.0	37.0	39.0	33.0	39.0
10	36.9252719948801	38.0	37.0	39.0	33.0	39.0
11	37.029326506795165	39.0	37.0	39.0	33.0	39.0
12	36.95203854986259	38.0	37.0	39.0	33.0	39.0
13	37.05217784135828	39.0	37.0	39.0	33.0	39.0
14	38.225576930316606	40.0	38.0	41.0	34.0	41.0
15	38.160599329894964	40.0	38.0	41.0	34.0	41.0
16	38.05665775703046	39.0	37.0	40.0	33.0	41.0
17	38.07830440838761	39.0	38.0	40.0	33.0	41.0
18	38.00598576967963	39.0	37.0	40.0	33.0	41.0
19	38.06313292926251	40.0	37.0	40.0	34.0	41.0
20	38.00583518427889	39.0	37.0	41.0	33.0	41.0
21	37.97473929902496	39.0	37.0	40.0	33.0	41.0
22	37.8905244136581	39.0	37.0	40.0	33.0	41.0
23	37.851598087565414	39.0	37.0	40.0	33.0	41.0
24	37.8127470541731	39.0	37.0	40.0	33.0	41.0
25	37.734743816586985	39.0	37.0	40.0	33.0	41.0
26	37.55607423860257	39.0	37.0	40.0	33.0	41.0
27	37.43598238150811	39.0	37.0	40.0	32.0	41.0
28	37.42506493995407	39.0	37.0	40.0	32.0	41.0
29	37.32353273350149	39.0	36.0	40.0	32.0	41.0
30	37.27399013665625	39.0	36.0	40.0	32.0	41.0
31	37.18401535971088	39.0	36.0	40.0	32.0	41.0
32	37.10823325678575	39.0	36.0	40.0	32.0	41.0
33	37.015811467078265	39.0	36.0	40.0	31.0	41.0
34	36.9178180175432	39.0	36.0	40.0	31.0	41.0
35	36.7757783382901	39.0	35.0	40.0	31.0	41.0
36	36.77389602078078	38.0	35.0	40.0	31.0	41.0
37	36.70248089447728	38.0	35.0	40.0	31.0	41.0
38	36.572789218085305	38.0	35.0	40.0	31.0	41.0
39	36.51387268004367	38.0	35.0	40.0	30.0	41.0
40	36.35959793698001	38.0	35.0	40.0	30.0	41.0
41	36.26371268305538	38.0	35.0	40.0	30.0	41.0
42	36.24884237473177	38.0	35.0	40.0	30.0	41.0
43	35.33678424876708	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	4.0
20	3.0
21	9.0
22	16.0
23	32.0
24	36.0
25	67.0
26	94.0
27	142.0
28	190.0
29	277.0
30	389.0
31	547.0
32	670.0
33	1010.0
34	1490.0
35	2345.0
36	3584.0
37	5333.0
38	6633.0
39	3690.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.297067349320486	16.22934156533524	11.753190528178294	28.72040055716598
2	21.95535142867899	18.725294582690207	31.122237699055077	28.197116289575725
3	22.147347814629374	19.128110529684147	27.63618567179912	31.08835598388736
4	16.696156307645975	15.284418175657871	32.443624590595945	35.575800926100214
5	18.258479840379476	32.14245378910515	32.31939163498099	17.27967473553439
6	36.41154990023717	32.33068554003689	14.56913752211723	16.688627037608704
7	32.00692692843429	26.292210970146446	18.808116553100177	22.89274554831909
8	28.58863833151376	30.214960659564056	18.988819033994655	22.207581974927532
9	27.952415013364458	12.555057787147536	16.270752550540223	43.221774648947786
10	19.97515340887701	22.779806497760042	28.791928622520047	28.453111470842902
11	40.3681813048225	18.845762903286527	18.051424914354552	22.734630877536425
12	24.089899484245002	22.471106426231977	25.565636411549903	27.87335767797312
13	34.99228249821179	16.970974664006324	19.98268267891428	28.0540601588676
14	24.978353348642848	18.41282987614351	22.090878289349845	34.517938485863795
15	30.11331551406091	23.208974889884427	18.90976169860332	27.76794789745134
16	27.094078229115688	23.31062003538757	21.051839024206604	28.54346271129014
17	27.628656401761848	23.284267590257123	21.194895154914732	27.892180853066296
18	26.969845273500738	20.74313895267854	22.644279637089184	29.642736136731546
19	29.34532997025938	21.590181831871398	21.6654745322441	27.399013665625116
20	29.209803109588528	20.396792530964124	22.211346609946165	28.182057749501187
21	28.889809133004558	20.78078530286489	21.24760004517562	29.08180551895494
22	29.16086285434627	22.08334901931258	20.04668147423107	28.709106652110076
23	29.285095809961227	21.804766027933592	21.187365884877458	27.722772277227726
24	28.746753002296426	20.724315777585364	21.729473327560893	28.799457892557317
25	29.364153145352557	20.71302187252946	22.128524639536195	27.794300342581785
26	29.08557015397357	22.113466099461657	21.533712306591877	27.267251439972895
27	28.75804690735233	21.097014644430224	22.55769303166058	27.587245416556865
28	27.69641983209728	22.26781613522569	21.228776870082445	28.806987162594588
29	28.110529684147124	22.7534540526296	21.458419606219177	27.677596657004106
30	28.076647968979408	21.055603659225238	22.77604186274141	28.09170650905395
31	28.351466325339757	21.21748296502654	21.880058728306288	28.55099198132741
32	26.962316003463467	21.258893950231524	22.388284455822006	29.390505590483002
33	27.406542935662387	21.737002597598163	23.088506569288107	27.76794789745134
34	28.151940669352104	21.349245190678765	22.294168580356136	28.204645559612995
35	27.564657606445053	22.07205511425667	23.107329744381282	27.25595753491699
36	27.33501487030832	21.213718330007904	23.047095584083124	28.40417121560065
37	27.376425855513308	20.53231939163498	23.5402627715243	28.55099198132741
38	26.47291345104092	20.381733990889582	24.846591122990628	28.298761435078866
39	25.742574257425744	20.0278582991379	25.576930316605807	28.652637126830555
40	25.89315965817114	20.381733990889582	26.732673267326735	26.992433083612543
41	24.504009336294846	19.60998381206942	27.79053570756315	28.095471144072583
42	23.152505364604902	20.38549862590822	28.212174829650266	28.249821179836616
43	22.44475398110153	20.25750103527463	28.313819975153407	28.98392500847043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.0
4	1.0
5	1.0
6	3.0
7	5.0
8	2.5
9	0.0
10	2.5
11	5.0
12	5.0
13	9.0
14	13.0
15	17.0
16	21.0
17	17.5
18	14.0
19	14.0
20	22.5
21	31.0
22	26.0
23	21.0
24	25.0
25	29.0
26	29.0
27	43.0
28	57.0
29	63.5
30	70.0
31	96.0
32	122.0
33	122.0
34	175.5
35	229.0
36	286.5
37	344.0
38	453.0
39	562.0
40	562.0
41	634.5
42	707.0
43	885.0
44	1063.0
45	1279.0
46	1495.0
47	1495.0
48	1774.0
49	2053.0
50	2307.5
51	2562.0
52	2682.5
53	2803.0
54	2803.0
55	2764.0
56	2725.0
57	2643.0
58	2561.0
59	2383.5
60	2206.0
61	2206.0
62	2150.0
63	2094.0
64	1698.5
65	1303.0
66	1156.5
67	1010.0
68	1010.0
69	881.0
70	752.0
71	653.0
72	554.0
73	518.5
74	483.0
75	483.0
76	433.0
77	383.0
78	270.0
79	157.0
80	100.5
81	44.0
82	44.0
83	42.0
84	40.0
85	32.0
86	24.0
87	17.0
88	10.0
89	10.0
90	7.5
91	5.0
92	3.0
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26563.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.64755486955539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.01819761795225	64.82325038587508
2	6.001124571895926	8.839363023754848
3	2.228697030107857	4.924142604374506
4	1.1603537289781731	3.418288596920529
5	0.6236262331953177	2.2964273613673156
6	0.5060573531666922	2.2361932010691565
7	0.2760312835454685	1.4230320370440086
8	0.23002606962122374	1.3552686067085797
9	0.15335071308081583	1.0164514550314347
>10	0.7872003271481879	9.035124044723863
>50	0.015335071308081582	0.6324586831306706
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	62	0.23340737115536647	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	53	0.199525655987652	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	53	0.199525655987652	TruSeq Adapter, Index 8 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	50	0.18823175093174718	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	45	0.16940857583857247	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	37	0.1392914956894929	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	31	0.11670368557768324	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	28	0.1054097805217784	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	28	0.1054097805217784	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	27	0.10164514550314346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0037646350186349435	0.0
26	0.0	0.0	0.0	0.01129390505590483	0.0
27	0.0	0.0	0.0	0.0263524451304446	0.0
28	0.0	0.0	0.0	0.12046832059631819	0.0
29	0.0	0.0	0.0	0.25599518126717613	0.0
30	0.0	0.0	0.0	0.4404622971802884	0.0
31	0.0	0.0	0.0	0.9072770394910213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCAAT	20	0.0018097443	37.0	30
GTCAATG	20	0.0018097443	37.0	31
AACGTCA	30	3.5064598E-4	30.833334	28
ACGTCAA	25	0.005401372	29.6	29
GGGTAAC	25	0.005401372	29.6	24
CGGGTAA	25	0.005401372	29.6	23
TCTGCGG	25	0.005401372	29.6	19
TGCGGGT	25	0.005401372	29.6	21
GCGGGTA	25	0.005401372	29.6	22
TCTTATA	50	0.0068598264	18.5	36
ACCTGTC	70	0.0025041865	15.857143	37
TATCAAC	70	0.0025041865	15.857143	1
ATCAACG	70	0.0025041865	15.857143	2
CTCTTAT	85	5.123325E-4	15.235293	37
AACGCAG	75	0.0039657345	14.8	5
ACGCAGA	75	0.0039657345	14.8	6
TCAACGC	75	0.0039657345	14.8	3
CGCAGAG	75	0.0039657345	14.8	7
CAACGCA	75	0.0039657345	14.8	4
CAGAGTA	80	0.006087554	13.875	9
>>END_MODULE