FastQCFastQC Report
Fri 10 Feb 2017
ERR1632700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612989
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8370.1365440489144177No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8230.1342601580126234No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7930.129366106080207No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7400.12071994766627134No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6670.10881108796405808No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6580.10734287238433315No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6510.106200926933436No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC6180.10081746980777795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5600.030.0624981
ATAATGC250.005494366429.63
ATGCGAT400.001930164423.1250027
ATACGGC753.7348764E-722.19999929
CTTATAC6350.021.55905537
GTATCAA7900.021.0759492
CATATAA450.00382380120.5555572
TAGCACT450.00382380120.5555574
AATAGAC450.00382380120.55555718
CTCTAAA450.00382380120.5555571
TATACAC1450.020.41379437
TATAACT555.139332E-420.1818184
GCGGTAA3150.019.38095323
CTTAATC500.007031226518.537
TAATACT500.007031226518.54
ACTATGA500.007031226518.51
CGTATTC500.007031226518.521
CTATATG500.007031226518.51
GTAGGGA701.2180195E-418.53
TATTCAA500.007031226518.510
Produced by FastQC (version 0.11.5)