##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632700.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	612989
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.060705820169694	34.0	33.0	34.0	31.0	34.0
2	33.19845380585949	34.0	33.0	34.0	31.0	34.0
3	33.27621213431236	34.0	34.0	34.0	31.0	34.0
4	36.555050743161786	37.0	37.0	37.0	35.0	37.0
5	36.52747276052262	37.0	37.0	37.0	35.0	37.0
6	36.57209672604239	37.0	37.0	37.0	35.0	37.0
7	36.56384861718563	37.0	37.0	37.0	35.0	37.0
8	36.53508464262817	37.0	37.0	37.0	35.0	37.0
9	38.33477925378759	39.0	39.0	39.0	37.0	39.0
10	38.36321206416429	39.0	39.0	39.0	37.0	39.0
11	38.43577943486751	39.0	39.0	39.0	37.0	39.0
12	38.403625513671535	39.0	39.0	39.0	37.0	39.0
13	38.43222798451522	39.0	39.0	39.0	37.0	39.0
14	40.00652377122591	41.0	40.0	41.0	38.0	41.0
15	40.00215338285026	41.0	40.0	41.0	38.0	41.0
16	39.97000924975815	41.0	40.0	41.0	38.0	41.0
17	39.964511598087405	41.0	40.0	41.0	38.0	41.0
18	39.956301010295455	41.0	40.0	41.0	38.0	41.0
19	39.968271861322144	41.0	40.0	41.0	38.0	41.0
20	39.93883903300059	41.0	40.0	41.0	38.0	41.0
21	39.90574382248295	41.0	40.0	41.0	38.0	41.0
22	39.88601100509145	41.0	40.0	41.0	38.0	41.0
23	39.84699236038493	41.0	40.0	41.0	38.0	41.0
24	39.83029874924346	41.0	40.0	41.0	38.0	41.0
25	39.787431748367425	41.0	40.0	41.0	38.0	41.0
26	39.706051821484564	41.0	40.0	41.0	38.0	41.0
27	39.63470470106315	41.0	40.0	41.0	37.0	41.0
28	39.59673501482082	41.0	40.0	41.0	37.0	41.0
29	39.55606707461308	41.0	40.0	41.0	37.0	41.0
30	39.51154425283325	41.0	40.0	41.0	37.0	41.0
31	39.456972311085515	41.0	40.0	41.0	37.0	41.0
32	39.40589472241753	41.0	39.0	41.0	36.0	41.0
33	39.34647440655542	41.0	39.0	41.0	36.0	41.0
34	39.31741189483009	41.0	39.0	41.0	36.0	41.0
35	39.23997167975282	41.0	39.0	41.0	35.0	41.0
36	39.190605377910536	41.0	39.0	41.0	35.0	41.0
37	39.1405979552651	41.0	39.0	41.0	35.0	41.0
38	39.06606806973698	41.0	39.0	41.0	35.0	41.0
39	39.00066069701088	41.0	39.0	41.0	35.0	41.0
40	38.929925333081016	40.0	39.0	41.0	35.0	41.0
41	38.88394734652661	40.0	39.0	41.0	35.0	41.0
42	38.825683658271195	40.0	38.0	41.0	35.0	41.0
43	38.10785837918788	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	11.0
19	18.0
20	37.0
21	66.0
22	117.0
23	219.0
24	330.0
25	498.0
26	667.0
27	893.0
28	1265.0
29	1772.0
30	2468.0
31	3261.0
32	4216.0
33	5863.0
34	8856.0
35	14062.0
36	22760.0
37	43364.0
38	115597.0
39	386645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17271598674691	16.12345409134585	12.580323627340784	28.123506294566457
2	20.67769568458814	19.05009714693086	32.682152534547924	27.59005463393307
3	20.904779694252262	19.571803082926447	28.67457654215655	30.84884068066474
4	16.058852605837952	15.047741476600724	32.258817042393915	36.63458887516742
5	17.104058963537682	33.2917882702626	33.05752631776427	16.546626448435454
6	36.58842165193829	32.32504987854595	14.704994706267161	16.38153376324861
7	31.60497170422308	27.211418149428457	19.614218199674056	21.56939194667441
8	28.096752144002586	31.357985216700463	19.584690753015146	20.96057188628181
9	28.071792479147263	13.265980303072322	17.085950971387742	41.57627624639267
10	19.079298323460943	24.278902231524548	29.90265730706424	26.73914213795027
11	39.514249032201235	19.22302031520957	18.755964625792632	22.506766026796566
12	23.49291749117847	23.26208137503283	26.035540605133207	27.209460528655487
13	34.251511854209454	17.349740370544986	21.266776402186665	27.131971373058896
14	24.722955876859128	18.974891882236058	22.349503824701586	33.95264841620323
15	29.049460920179644	24.307777137925804	19.73966906420833	26.903092877686223
16	27.060028809652376	23.388674185018	21.46041772364594	28.090879281683684
17	27.431161081193956	23.49536451714468	21.429911466600544	27.64356293506082
18	26.690854158883763	21.832691940638412	23.122600894958964	28.353853005518857
19	28.71128193164967	22.049824711373287	22.673000657434308	26.565892699542733
20	29.404279685279832	21.11538706241058	22.751468623417388	26.728864628892197
21	27.372758728133782	22.637763483520914	21.64133451008093	28.348143278264377
22	28.42905827021366	22.368753762302422	21.042139418488752	28.16004854899517
23	27.842424578581344	22.55782730195811	21.94900724156551	27.650740877895036
24	28.348632683457613	21.987180846638356	21.85650966004284	27.80767680986119
25	28.69317393949973	21.812789462779918	22.49518343722318	26.99885316049717
26	28.31616880563925	22.25847445875864	22.21720128746193	27.208155448140182
27	27.387440883931035	21.710014372199176	23.2229289595735	27.679615784296292
28	26.69232237446349	22.43123449197294	22.320139513107087	28.556303620456486
29	27.58255042097003	23.119827598863925	21.987833386896014	27.309788593270024
30	27.089066851118048	22.660112987345613	22.683441301556797	27.56737885997954
31	27.97847922230252	22.14460618379775	22.502198244992975	27.37471634890675
32	25.930807893779495	22.176743791487286	22.801714223256862	29.090734091476357
33	26.52706655421223	21.6080549569405	23.779872069482487	28.085006419364785
34	26.866713758321925	21.575754214186553	23.678402059417053	27.879129968074466
35	26.444683346683217	22.43629167896977	24.056875408857255	27.062149565489758
36	26.373393323534355	21.713440208551866	24.647261206971088	27.26590526094269
37	26.77813141834519	20.779165694653575	24.665206064056612	27.77749682294462
38	25.551029463823983	20.69107275987008	26.06001086479529	27.69788691151065
39	25.117579597676304	19.751741058974957	26.906029308845675	28.224650034503068
40	24.63714683297743	20.349794205116243	28.094957658294035	26.918101303612303
41	23.264854671127864	20.157621099236692	28.736078461440577	27.841445768194863
42	22.46581912562868	20.34636836876355	29.36219083866105	27.825621666946716
43	21.548673793493847	20.680632115747592	29.4238558930095	28.346838197749065
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	7.0
4	13.0
5	13.0
6	18.0
7	23.0
8	24.0
9	25.0
10	31.0
11	37.0
12	37.0
13	81.0
14	125.0
15	248.0
16	371.0
17	379.5
18	388.0
19	388.0
20	466.0
21	544.0
22	576.0
23	608.0
24	749.5
25	891.0
26	891.0
27	1129.5
28	1368.0
29	2233.0
30	3098.0
31	3691.5
32	4285.0
33	4285.0
34	5666.5
35	7048.0
36	8010.0
37	8972.0
38	12424.5
39	15877.0
40	15877.0
41	19017.0
42	22157.0
43	24574.5
44	26992.0
45	34341.0
46	41690.0
47	41690.0
48	46767.0
49	51844.0
50	58474.0
51	65104.0
52	69026.5
53	72949.0
54	72949.0
55	65979.0
56	59009.0
57	55665.5
58	52322.0
59	48630.0
60	44938.0
61	44938.0
62	41911.0
63	38884.0
64	33551.5
65	28219.0
66	24917.5
67	21616.0
68	21616.0
69	18617.0
70	15618.0
71	13296.0
72	10974.0
73	8915.0
74	6856.0
75	6856.0
76	5550.5
77	4245.0
78	3569.5
79	2894.0
80	2163.5
81	1433.0
82	1433.0
83	1132.5
84	832.0
85	619.0
86	406.0
87	302.0
88	198.0
89	198.0
90	144.5
91	91.0
92	59.5
93	28.0
94	16.5
95	5.0
96	5.0
97	6.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	612989.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.86621451321295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.79865137668784	44.829836941111395
2	7.747105579771101	8.191202908733759
3	2.4623677571205924	3.905281861750688
4	1.209289303231748	2.5572219085273367
5	0.7532358142299885	1.9910363067058596
6	0.472648495330312	1.4992282046087764
7	0.34947600223498576	1.2932831310962392
8	0.25995653022945603	1.0994334152976757
9	0.206068285024752	0.98046451444396
>10	1.4578934586555343	15.212508796641039
>50	0.16794314311160163	6.243249699348339
>100	0.1110342287388469	10.722895111111427
>500	0.004330025633269775	1.474357200623536
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	837	0.1365440489144177	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	823	0.1342601580126234	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	793	0.129366106080207	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	740	0.12071994766627134	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	667	0.10881108796405808	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	658	0.10734287238433315	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	651	0.106200926933436	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	618	0.10081746980777795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.6313506441388017E-4	0.0
4	0.0	0.0	0.0	1.6313506441388017E-4	0.0
5	0.0	0.0	0.0	1.6313506441388017E-4	0.0
6	0.0	0.0	0.0	1.6313506441388017E-4	0.0
7	0.0	0.0	0.0	1.6313506441388017E-4	0.0
8	1.6313506441388017E-4	0.0	0.0	1.6313506441388017E-4	0.0
9	1.6313506441388017E-4	0.0	0.0	1.6313506441388017E-4	0.0
10	1.6313506441388017E-4	0.0	0.0	1.6313506441388017E-4	0.0
11	1.6313506441388017E-4	0.0	0.0	1.6313506441388017E-4	0.0
12	1.6313506441388017E-4	0.0	0.0	1.6313506441388017E-4	0.0
13	1.6313506441388017E-4	0.0	0.0	3.2627012882776034E-4	0.0
14	1.6313506441388017E-4	0.0	0.0	3.2627012882776034E-4	0.0
15	1.6313506441388017E-4	0.0	0.0	3.2627012882776034E-4	0.0
16	1.6313506441388017E-4	0.0	0.0	6.525402576555207E-4	0.0
17	1.6313506441388017E-4	0.0	0.0	8.15675322069401E-4	0.0
18	1.6313506441388017E-4	0.0	0.0	8.15675322069401E-4	0.0
19	1.6313506441388017E-4	0.0	0.0	0.0011419454508971613	0.0
20	3.2627012882776034E-4	0.0	0.0	0.0013050805153110414	0.0
21	3.2627012882776034E-4	0.0	0.0	0.0024470259662082027	0.0
22	3.2627012882776034E-4	0.0	0.0	0.004894051932416405	0.0
23	3.2627012882776034E-4	0.0	0.0	0.006035997383313567	0.0
24	3.2627012882776034E-4	0.0	0.0	0.007830483091866248	0.0
25	3.2627012882776034E-4	0.0	0.0	0.00815675322069401	0.0
26	3.2627012882776034E-4	0.0	0.0	0.011419454508971612	0.0
27	3.2627012882776034E-4	0.0	0.0	0.031158797303051114	0.0
28	3.2627012882776034E-4	0.0	0.0	0.12235129831041014	0.0
29	3.2627012882776034E-4	0.0	0.0	0.2708042069270411	0.0
30	3.2627012882776034E-4	0.0	0.0	0.4554730998435535	0.0
31	3.2627012882776034E-4	0.0	0.0	1.0518948953406995	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	560	0.0	30.062498	1
ATAATGC	25	0.0054943664	29.6	3
ATGCGAT	40	0.0019301644	23.125002	7
ATACGGC	75	3.7348764E-7	22.199999	29
CTTATAC	635	0.0	21.559055	37
GTATCAA	790	0.0	21.075949	2
CATATAA	45	0.003823801	20.555557	2
TAGCACT	45	0.003823801	20.555557	4
AATAGAC	45	0.003823801	20.555557	18
CTCTAAA	45	0.003823801	20.555557	1
TATACAC	145	0.0	20.413794	37
TATAACT	55	5.139332E-4	20.181818	4
GCGGTAA	315	0.0	19.380953	23
CTTAATC	50	0.0070312265	18.5	37
TAATACT	50	0.0070312265	18.5	4
ACTATGA	50	0.0070312265	18.5	1
CGTATTC	50	0.0070312265	18.5	21
CTATATG	50	0.0070312265	18.5	1
GTAGGGA	70	1.2180195E-4	18.5	3
TATTCAA	50	0.0070312265	18.5	10
>>END_MODULE