FastQCFastQC Report
Fri 10 Feb 2017
ERR1632699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16068
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT950.5912372417226786Illumina PCR Primer Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA830.5165546427682349No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA650.40453074433656955No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA640.3983071944236993No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA640.3983071944236993No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT500.31117749564351505No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA490.30495394573064477No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC430.26761264625342296No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC410.25516554642768235No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT400.24894199651481203No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC360.22404779686333084No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC360.22404779686333084No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA360.22404779686333084No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT350.21782424695046051No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC350.21782424695046051No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA340.21160069703759027No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT310.19293004729897933No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG310.19293004729897933No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC300.18670649738610903No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA300.18670649738610903No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC290.18048294747323873No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC290.18048294747323873No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT290.18048294747323873No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG280.17425939756036843No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT280.17425939756036843No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA280.17425939756036843No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA270.16803584764749813No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC270.16803584764749813No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG270.16803584764749813No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC260.16181229773462785No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC260.16181229773462785No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT260.16181229773462785No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC260.16181229773462785No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC250.15558874782175752Illumina PCR Primer Index 10 (95% over 22bp)
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC250.15558874782175752No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA250.15558874782175752No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA250.15558874782175752No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC240.14936519790888725No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT240.14936519790888725No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC230.14314164799601692No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC230.14314164799601692No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA230.14314164799601692No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG220.13691809808314662No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC220.13691809808314662No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC220.13691809808314662No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG220.13691809808314662No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC210.13069454817027634No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC210.13069454817027634No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA210.13069454817027634No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT210.13069454817027634No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG210.13069454817027634No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG210.13069454817027634No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA200.12447099825740601No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG200.12447099825740601No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA200.12447099825740601No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA200.12447099825740601No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC190.11824744834453572No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG190.11824744834453572No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA190.11824744834453572No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT180.11202389843166542No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG180.11202389843166542No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA180.11202389843166542No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG180.11202389843166542No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC180.11202389843166542No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG180.11202389843166542No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT180.11202389843166542No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG180.11202389843166542No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA170.10580034851879513No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT170.10580034851879513No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA170.10580034851879513No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC170.10580034851879513No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT170.10580034851879513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT170.10580034851879513No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA170.10580034851879513No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC170.10580034851879513No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC170.10580034851879513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA200.001788398737.01
CTCTTAT603.4406097E-521.58333437
TCTCTTA1009.907434E-514.837
GTCTCTT1256.561644E-411.8436
TGTCTCT1758.118812E-510.57142937
CTGTCTC2350.00156190077.872340736
Produced by FastQC (version 0.11.5)