FastQCFastQC Report
Fri 10 Feb 2017
ERR1632698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60656
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT2590.4269981535214983TruSeq Adapter, Index 3 (95% over 21bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2400.3956739646531258No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2160.3561065681878133No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1950.32148509628066474No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1940.3198364547612767No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1770.2918095489316803No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1560.2571880770245318No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1550.2555394355051438No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1500.24729622790820366No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1450.23905302031126352No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1340.22091796359799526No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1320.21762068055921918No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1250.20608018992350302No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1200.1978369823265629No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1140.18794513321023476No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1100.1813505671326827No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1070.1764046425745186No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1060.17475600105513056No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1040.1714587180163545No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1040.1714587180163545No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1000.16486415193880244No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1000.16486415193880244No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG960.1582695858612503No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA910.15002637826431023No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT890.14672909522553415No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA860.1417831706673701No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA860.1417831706673701No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC850.14013452914798205No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC840.13848588762859404No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC830.13683724610920603No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC790.1302426800316539No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG770.12694539699287785No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC770.12694539699287785No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA760.12529675547348984No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT760.12529675547348984No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG760.12529675547348984No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA740.12199947243471379No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC730.12035083091532578No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA720.11870218939593775No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC710.11705354787654972No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC680.11210762331838565No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA670.11045898179899763No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC670.11045898179899763No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC660.10881034027960959No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC650.10716169876022158No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC630.10386441572144553No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA620.1022157742020575No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC620.1022157742020575No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA610.10056713268266947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050.033.4761921
TAATACG1000.033.30000327
AATACGG1000.031.4528
ATACGGA658.367351E-1131.30769229
TACACAT250.00545589729.65
ACACATC250.00545589729.66
GTGTGAG250.00545589729.61
TACGGAG701.9826984E-1029.07142830
ACGGAGG701.9826984E-1029.07142831
CGGAGGG652.8721843E-928.46153832
GCGGTAA1250.028.12000323
TGCCAGC1250.028.12000312
CGTGCCA1250.028.12000310
AGCCGCG1250.028.12000319
GGTAATA1250.028.12000325
TACGGCT405.849277E-527.75000230
AGGGTGC604.1825842E-827.75000235
TCTTATA1350.027.40740637
CGCGGTA1300.027.03846222
GTAATAC1300.027.03846226
Produced by FastQC (version 0.11.5)