FastQCFastQC Report
Fri 10 Feb 2017
ERR1632697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851735
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14010.1644877808238478No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10750.1262129653002401No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10050.11799444662952678No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9190.10789740940550759No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8620.10120518705935531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.029.3316041
TATACTT405.938351E-527.755
CTTATAC10150.021.68965537
GCGGTAA4800.021.19791623
CGTTAGA450.003825006520.5555551
TAAGGTA450.003825006520.5555555
TACTAGG450.003825006520.5555552
TAATACG5000.019.6127
CGGTAAT5200.019.56730824
GTATCAA14650.019.3208182
GTAATAC5400.018.84259226
GGTAATA5550.018.66666625
CGCGGTA5550.018.66666622
ATAACGC701.218727E-418.535
AACTCCG5850.018.341885
TAACTCC5650.018.3362834
CTAACTC5550.018.3333343
TCTATGG1850.018.02
AGCCGCG5800.017.8620719
GCTAACT5600.017.8392852
Produced by FastQC (version 0.11.5)