##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632697.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	851735
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04457959341814	34.0	33.0	34.0	31.0	34.0
2	33.187718010883664	34.0	33.0	34.0	31.0	34.0
3	33.2626626826419	34.0	34.0	34.0	31.0	34.0
4	36.54848280274968	37.0	37.0	37.0	35.0	37.0
5	36.514537972491446	37.0	37.0	37.0	35.0	37.0
6	36.557433943656186	37.0	37.0	37.0	35.0	37.0
7	36.55046111760113	37.0	37.0	37.0	35.0	37.0
8	36.52432740230236	37.0	37.0	37.0	35.0	37.0
9	38.31627090585687	39.0	39.0	39.0	37.0	39.0
10	38.35051982130592	39.0	39.0	39.0	37.0	39.0
11	38.42226279300487	39.0	39.0	39.0	37.0	39.0
12	38.384548010824965	39.0	39.0	39.0	37.0	39.0
13	38.41500936324091	39.0	39.0	39.0	37.0	39.0
14	39.97647507734213	41.0	40.0	41.0	38.0	41.0
15	39.96694981420278	41.0	40.0	41.0	38.0	41.0
16	39.937023839574515	41.0	40.0	41.0	38.0	41.0
17	39.93215965059555	41.0	40.0	41.0	38.0	41.0
18	39.91990231703523	41.0	40.0	41.0	38.0	41.0
19	39.92865738756773	41.0	40.0	41.0	38.0	41.0
20	39.89478652397753	41.0	40.0	41.0	38.0	41.0
21	39.867488127175704	41.0	40.0	41.0	38.0	41.0
22	39.83843918589702	41.0	40.0	41.0	38.0	41.0
23	39.79120618502234	41.0	40.0	41.0	38.0	41.0
24	39.772954029128776	41.0	40.0	41.0	38.0	41.0
25	39.72642312456339	41.0	40.0	41.0	38.0	41.0
26	39.64359454525175	41.0	40.0	41.0	37.0	41.0
27	39.574831373607985	41.0	40.0	41.0	37.0	41.0
28	39.52388242822005	41.0	40.0	41.0	37.0	41.0
29	39.48248574967566	41.0	40.0	41.0	37.0	41.0
30	39.431955948739926	41.0	39.0	41.0	37.0	41.0
31	39.37337434765508	41.0	39.0	41.0	36.0	41.0
32	39.31880338368154	41.0	39.0	41.0	36.0	41.0
33	39.253308834320535	41.0	39.0	41.0	36.0	41.0
34	39.21209765948329	41.0	39.0	41.0	35.0	41.0
35	39.1391876581331	41.0	39.0	41.0	35.0	41.0
36	39.08819527200362	41.0	39.0	41.0	35.0	41.0
37	39.032547682084214	41.0	39.0	41.0	35.0	41.0
38	38.954145362113806	41.0	39.0	41.0	35.0	41.0
39	38.86954393091748	40.0	39.0	41.0	35.0	41.0
40	38.79327020728278	40.0	38.0	41.0	35.0	41.0
41	38.740267806301254	40.0	38.0	41.0	35.0	41.0
42	38.67041626797067	40.0	38.0	41.0	35.0	41.0
43	37.930589913529445	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	8.0
18	15.0
19	48.0
20	68.0
21	96.0
22	203.0
23	305.0
24	558.0
25	797.0
26	1064.0
27	1400.0
28	1983.0
29	2712.0
30	3644.0
31	4875.0
32	6298.0
33	8519.0
34	12911.0
35	20640.0
36	33282.0
37	63642.0
38	166672.0
39	521993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4945141387873	16.055932889924684	12.269191708688735	28.180361262599284
2	21.214462244712266	18.771566273547524	32.047409111989055	27.966562369751152
3	21.244048911926832	19.279940357035933	28.08796163125855	31.388049099778687
4	16.257169189947575	15.043176574873641	32.27881911627443	36.42083511890436
5	17.75857514367732	32.42252578560233	32.69631986474666	17.12257920597369
6	36.55327067691242	32.17326985506055	14.607360270506673	16.666099197520357
7	31.772793180977654	27.018262722560422	19.201747022254576	22.00719707420735
8	28.360112006668743	30.605763529736365	19.45088554538677	21.583238918208128
9	28.321132746687645	12.906126905669016	16.987325870135663	41.78541447750768
10	19.399108877761275	23.3712363587266	29.833340182098894	27.396314581413233
11	39.64331628969104	19.077882205145965	18.545557010102907	22.733244495060084
12	23.788737107198834	22.951035239833985	26.180208632966828	27.08001902000035
13	34.94819398052211	17.1391336507247	20.82748742273125	27.085184946021947
14	24.86912009016889	18.840014793333605	22.44864893423424	33.842216182263265
15	29.434624619159717	23.500149694447217	19.662805919681592	27.40241976671148
16	26.943121980428188	23.13982635444123	21.59732780735792	28.319723857772665
17	27.50996495388824	22.958901536275956	21.653448549137934	27.87768496069787
18	27.190029762778327	21.378715210716948	22.97886079590483	28.452394230599893
19	28.85909349739062	21.741151884095405	22.461915971516962	26.937838646997015
20	29.60932684461716	21.005594463066565	22.263262634504862	27.121816057811408
21	27.96409681414994	21.8670126271669	21.53263632467845	28.63625423400471
22	28.879404979248243	22.43056819315867	20.73696630994382	27.95306051764927
23	28.73229349504247	21.886619664567032	21.710743365013766	27.67034347537673
24	28.4006175629744	21.44469817490182	21.85315855283627	28.30152570928751
25	28.77502979213018	21.645464845286387	22.152195225040654	27.427310137542783
26	28.35353719173217	22.264084486371935	21.930177813521812	27.452200508374087
27	27.888134220150633	21.57114595502122	22.87448560878677	27.666234216041374
28	27.207523466805988	22.3179744873699	21.849049293500915	28.6254527523232
29	27.743488291546083	22.735592643251714	21.837425959952334	27.683493105249873
30	27.420030878148722	22.196575225862507	22.736649309937953	27.646744586050826
31	28.045812371218748	21.906813739015067	22.060265223338245	27.98710866642794
32	26.249948634258306	21.887793738662847	22.662917456720695	29.199340170358152
33	26.732962717277086	21.610360029821482	23.66592895677646	27.990748296124966
34	27.079314575542863	21.057018908463313	23.55991006592426	28.30375645006956
35	26.659700493698157	22.143741891550775	24.118886742942347	27.077670871808717
36	26.661226790022717	21.12593705788772	24.482027860778295	27.730808291311266
37	26.857531978843184	20.574709269902023	24.49905193516763	28.068706816087165
38	25.810375292784727	20.216616670678086	25.723141587465587	28.249866449071604
39	25.321373431877287	19.480237397782176	26.876317164376246	28.322072005964294
40	24.965041943797072	20.190317410931804	27.822268663375343	27.022371981895777
41	23.666985623462697	19.670437401304397	28.540919417424433	28.121657557808472
42	22.55819004737389	20.210746300199006	29.268493134601727	27.96257051782538
43	21.84447040452723	20.46986445314564	29.196404985118612	28.489260157208523
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	9.5
2	8.0
3	18.0
4	28.0
5	28.0
6	40.0
7	52.0
8	54.0
9	56.0
10	69.0
11	82.0
12	82.0
13	198.5
14	315.0
15	508.5
16	702.0
17	685.0
18	668.0
19	668.0
20	812.0
21	956.0
22	991.0
23	1026.0
24	1254.5
25	1483.0
26	1483.0
27	1863.0
28	2243.0
29	3096.0
30	3949.0
31	5022.0
32	6095.0
33	6095.0
34	7858.0
35	9621.0
36	11357.5
37	13094.0
38	16351.5
39	19609.0
40	19609.0
41	22946.0
42	26283.0
43	30661.5
44	35040.0
45	44314.5
46	53589.0
47	53589.0
48	60054.0
49	66519.0
50	74644.0
51	82769.0
52	87907.5
53	93046.0
54	93046.0
55	89124.5
56	85203.0
57	81834.5
58	78466.0
59	73847.0
60	69228.0
61	69228.0
62	65067.0
63	60906.0
64	52200.0
65	43494.0
66	38053.0
67	32612.0
68	32612.0
69	27885.5
70	23159.0
71	19951.0
72	16743.0
73	13494.0
74	10245.0
75	10245.0
76	8117.5
77	5990.0
78	4919.0
79	3848.0
80	3029.0
81	2210.0
82	2210.0
83	1699.0
84	1188.0
85	908.0
86	628.0
87	470.0
88	312.0
89	312.0
90	242.0
91	172.0
92	116.0
93	60.0
94	39.0
95	18.0
96	18.0
97	12.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	851735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.895231600428396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.017284218915	48.07956023056128
2	7.466626919352633	8.34697681862417
3	2.219573005503001	3.721906415899475
4	1.0406820190944248	2.3267664991873724
5	0.6310151849071332	1.7635369951885675
6	0.42923547658069683	1.4395329824758984
7	0.2914896258042035	1.1405016100413712
8	0.2319764093452301	1.0373100100949935
9	0.1748959315103818	0.8798263737970909
>10	1.2581013845988265	14.018808410877101
>50	0.14279676868840324	5.5732113911891
>100	0.08998601256413158	9.221634066338463
>500	0.005703338822335043	2.039425952816772
>1k	6.337043135927825E-4	0.4110022429083069
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1401	0.1644877808238478	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1075	0.1262129653002401	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1005	0.11799444662952678	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	919	0.10789740940550759	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	862	0.10120518705935531	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.348148191632374E-4	0.0	0.0	0.0	0.0
6	2.348148191632374E-4	0.0	0.0	0.0	0.0
7	2.348148191632374E-4	0.0	0.0	0.0	0.0
8	2.348148191632374E-4	0.0	0.0	0.0	0.0
9	2.348148191632374E-4	0.0	0.0	0.0	0.0
10	2.348148191632374E-4	0.0	0.0	1.174074095816187E-4	0.0
11	2.348148191632374E-4	0.0	0.0	1.174074095816187E-4	0.0
12	2.348148191632374E-4	0.0	0.0	1.174074095816187E-4	0.0
13	2.348148191632374E-4	0.0	0.0	1.174074095816187E-4	0.0
14	2.348148191632374E-4	0.0	0.0	1.174074095816187E-4	0.0
15	3.522222287448561E-4	0.0	0.0	1.174074095816187E-4	0.0
16	3.522222287448561E-4	0.0	0.0	1.174074095816187E-4	0.0
17	4.696296383264748E-4	0.0	0.0	3.522222287448561E-4	0.0
18	4.696296383264748E-4	0.0	0.0	4.696296383264748E-4	0.0
19	4.696296383264748E-4	0.0	0.0	5.870370479080935E-4	0.0
20	4.696296383264748E-4	0.0	0.0	0.0010566666862345682	0.0
21	4.696296383264748E-4	0.0	0.0	0.0028177778299588487	0.0
22	4.696296383264748E-4	0.0	0.0	0.0037570371066117983	0.0
23	4.696296383264748E-4	0.0	0.0	0.00446148156410151	0.0
24	4.696296383264748E-4	0.0	0.0	0.00540074084075446	0.0
25	4.696296383264748E-4	0.0	0.0	0.006222592707825791	0.0
26	4.696296383264748E-4	0.0	0.0	0.009275185356947878	0.0
27	4.696296383264748E-4	0.0	0.0	0.03322629691159809	0.0
28	4.696296383264748E-4	0.0	0.0	0.12644778011940333	0.0
29	4.696296383264748E-4	0.0	0.0	0.28353889413960914	0.0
30	4.696296383264748E-4	0.0	0.0	0.4880626016307889	0.0
31	4.696296383264748E-4	0.0	0.0	1.1417870581812417	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	965	0.0	29.331604	1
TATACTT	40	5.938351E-5	27.75	5
CTTATAC	1015	0.0	21.689655	37
GCGGTAA	480	0.0	21.197916	23
CGTTAGA	45	0.0038250065	20.555555	1
TAAGGTA	45	0.0038250065	20.555555	5
TACTAGG	45	0.0038250065	20.555555	2
TAATACG	500	0.0	19.61	27
CGGTAAT	520	0.0	19.567308	24
GTATCAA	1465	0.0	19.320818	2
GTAATAC	540	0.0	18.842592	26
GGTAATA	555	0.0	18.666666	25
CGCGGTA	555	0.0	18.666666	22
ATAACGC	70	1.218727E-4	18.5	35
AACTCCG	585	0.0	18.34188	5
TAACTCC	565	0.0	18.336283	4
CTAACTC	555	0.0	18.333334	3
TCTATGG	185	0.0	18.0	2
AGCCGCG	580	0.0	17.86207	19
GCTAACT	560	0.0	17.839285	2
>>END_MODULE