FastQCFastQC Report
Fri 10 Feb 2017
ERR1632696.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632696.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575821
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA32970.2092242710307833No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA27350.17356032188935164No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA26230.16645291565476028No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA23430.14868440006828185No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23300.14785943327319537No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC22090.1401808961804672No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA20490.13002745870247953No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA20240.12844098409654398No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG20100.12755255831722004No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT19510.1238084782472121No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT18620.11816062865008146No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC18230.11568572826482196No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT17550.11137051733667719No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA16860.10699184742429503No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16640.10559574977107172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.031.6721
ATACGGC1900.030.18421229
TACGGCT2550.022.49019630
GTATCAA27150.021.7366492
GTTATCG450.003826429120.55555511
GCGGTAA13350.019.81647923
TAATACG13150.018.85171127
ACCATAC609.23889E-418.58
TTTTACG1103.8542566E-818.54
CTTATAC17300.018.39306337
TATACAC5450.018.33027537
CGCGGTA14600.018.11986422
CGTGCCA15300.018.0163410
AACTCCG15400.017.8993535
GGTAATA14800.017.87525
CGGTAAT14800.017.87524
AGCCGCG15750.017.38412719
GTCTATA752.06788E-417.2666662
CCGCGGT16050.017.05918921
TCTATGG5000.017.022
Produced by FastQC (version 0.11.5)