##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632696.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1575821
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05377260488342	34.0	33.0	34.0	31.0	34.0
2	33.18857344838024	34.0	33.0	34.0	31.0	34.0
3	33.260994744961515	34.0	34.0	34.0	31.0	34.0
4	36.54267648419459	37.0	37.0	37.0	35.0	37.0
5	36.51452925173608	37.0	37.0	37.0	35.0	37.0
6	36.560639818862676	37.0	37.0	37.0	35.0	37.0
7	36.56048117140208	37.0	37.0	37.0	35.0	37.0
8	36.53520165044126	37.0	37.0	37.0	35.0	37.0
9	38.330477890572595	39.0	39.0	39.0	37.0	39.0
10	38.36234826163631	39.0	39.0	39.0	37.0	39.0
11	38.433745330212	39.0	39.0	39.0	37.0	39.0
12	38.39612049845763	39.0	39.0	39.0	37.0	39.0
13	38.426404394915416	39.0	39.0	39.0	37.0	39.0
14	39.98921768398822	41.0	40.0	41.0	38.0	41.0
15	39.98327094257533	41.0	40.0	41.0	38.0	41.0
16	39.947260507379966	41.0	40.0	41.0	38.0	41.0
17	39.949479033468904	41.0	40.0	41.0	38.0	41.0
18	39.94614299466754	41.0	40.0	41.0	38.0	41.0
19	39.953200268304585	41.0	40.0	41.0	38.0	41.0
20	39.926154683812435	41.0	40.0	41.0	38.0	41.0
21	39.892023903730184	41.0	40.0	41.0	38.0	41.0
22	39.866184039938545	41.0	40.0	41.0	38.0	41.0
23	39.828136571349155	41.0	40.0	41.0	38.0	41.0
24	39.806264797841884	41.0	40.0	41.0	38.0	41.0
25	39.76715502585636	41.0	40.0	41.0	38.0	41.0
26	39.681758270768064	41.0	40.0	41.0	38.0	41.0
27	39.60595461032693	41.0	40.0	41.0	37.0	41.0
28	39.565298977485384	41.0	40.0	41.0	37.0	41.0
29	39.53340131905845	41.0	40.0	41.0	37.0	41.0
30	39.48620687248108	41.0	40.0	41.0	37.0	41.0
31	39.42968458981065	41.0	39.0	41.0	37.0	41.0
32	39.381666445617874	41.0	39.0	41.0	36.0	41.0
33	39.323251816037484	41.0	39.0	41.0	36.0	41.0
34	39.28097734450804	41.0	39.0	41.0	36.0	41.0
35	39.20636671297057	41.0	39.0	41.0	35.0	41.0
36	39.15833524239111	41.0	39.0	41.0	35.0	41.0
37	39.10296283651506	41.0	39.0	41.0	35.0	41.0
38	39.029006467105084	41.0	39.0	41.0	35.0	41.0
39	38.94417132402729	41.0	39.0	41.0	35.0	41.0
40	38.87113257152938	40.0	39.0	41.0	35.0	41.0
41	38.81749323051286	40.0	38.0	41.0	35.0	41.0
42	38.75172687760856	40.0	38.0	41.0	35.0	41.0
43	38.03296440395197	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.0
17	8.0
18	39.0
19	63.0
20	116.0
21	221.0
22	312.0
23	544.0
24	913.0
25	1324.0
26	1842.0
27	2513.0
28	3411.0
29	4755.0
30	6544.0
31	8740.0
32	11348.0
33	15566.0
34	23185.0
35	37025.0
36	58921.0
37	113629.0
38	298502.0
39	986293.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.37427918526279	15.92357253774382	12.464740601883081	28.23740767511031
2	21.21034051456352	19.03331660131449	32.10307515891716	27.653267725204827
3	21.390119816908136	19.386783143516936	28.525701840500922	30.697395199074005
4	16.01711108050978	15.526382755401787	32.888951219713405	35.56755494437503
5	17.52680031551807	32.47735624794948	32.859125497121816	17.13671793941063
6	35.25501944700572	32.585426898105815	15.20420149242839	16.955352162460077
7	31.13767363171325	27.031052384756897	19.602670607892648	22.228603375637206
8	27.69502373683305	30.550360732595898	19.951187349324577	21.803428181246474
9	28.293568876160425	12.891946483769413	17.339025181159535	41.47545945891062
10	19.57582745756022	23.29331821317269	29.67735548644167	27.453498842825425
11	39.23510347939265	19.389638797807617	18.583519321039635	22.7917384017601
12	23.06480241093373	23.17338073296396	26.57027670020897	27.19154015589334
13	34.79049968238778	17.49532465933631	21.017425202481753	26.696750455794156
14	24.672599235573074	19.647980322638166	22.611007214651917	33.06841322713684
15	29.62614408616207	23.706372741574075	19.796728181690685	26.87075499057317
16	26.118766027359708	23.53021060133099	22.15372177423705	28.197301597072254
17	27.041459658171835	23.348337152506534	21.795749644153744	27.814453545167883
18	26.736095026021356	21.47769321515578	23.298839144801345	28.487372614021517
19	28.482486272235235	22.062658131856345	22.931157790129717	26.523697805778703
20	29.776288042867815	20.873373308262806	22.494623437560485	26.8557152113089
21	27.720090035606837	22.004529702294867	21.74859961886534	28.526780643232957
22	28.369275444355672	22.654476618854552	21.05423141333946	27.922016523450317
23	28.470555983198597	22.038036045972227	21.951160696551195	27.54024727427798
24	28.021075997844935	21.625044976555078	22.343210301169993	28.010668724429994
25	28.49663762572018	21.81485079840921	22.58092765612338	27.10758391974723
26	28.15516483153861	22.56049386319893	22.134366784044634	27.14997452121783
27	27.551479514488005	21.685838683454527	23.37924167783016	27.38344012422731
28	26.758686424409877	22.707401411708563	21.913719895851113	28.620192268030443
29	27.463779198271887	23.066135049602714	22.015381188599466	27.454704563525933
30	27.26546987252994	22.191226033921367	23.190641576676537	27.352662516872154
31	28.0908808805061	22.118565496969516	22.20912146747632	27.58143215504807
32	26.016470144768984	22.019125268669473	22.771558444772598	29.192846141788948
33	26.368032917444307	21.72080458376935	24.062377643146018	27.84878485564033
34	26.68202797145107	21.49520789480531	23.671787595164677	28.150976538578938
35	26.34125322609611	22.72504300932657	24.3228133144564	26.610890450120923
36	26.466965473870445	21.56044373060138	24.669235909408492	27.303354886119678
37	26.86313991246468	21.07758431953883	24.49821394688864	27.561061821107852
38	25.762761125787765	20.60354570728528	25.702728926699166	27.93096424022779
39	25.37223453679066	19.839880290972133	26.668765043745452	28.119120128491755
40	24.92783127017599	20.609637769772075	27.606308076869134	26.856222883182795
41	23.469480353415776	20.030003407747454	28.462242856263497	28.038273382573276
42	22.294029588385992	20.566866414396053	29.17825057541434	27.960853421803616
43	21.651507372982085	21.07409407540577	28.903028960776638	28.371369590835506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	6.0
2	8.0
3	30.5
4	53.0
5	53.0
6	68.5
7	84.0
8	90.0
9	96.0
10	146.0
11	196.0
12	196.0
13	361.0
14	526.0
15	978.5
16	1431.0
17	1443.0
18	1455.0
19	1455.0
20	1790.0
21	2125.0
22	1952.0
23	1779.0
24	2171.0
25	2563.0
26	2563.0
27	3142.5
28	3722.0
29	5215.5
30	6709.0
31	8579.0
32	10449.0
33	10449.0
34	13647.0
35	16845.0
36	19743.0
37	22641.0
38	28914.5
39	35188.0
40	35188.0
41	42444.5
42	49701.0
43	58049.0
44	66397.0
45	88035.0
46	109673.0
47	109673.0
48	122820.0
49	135967.0
50	152630.5
51	169294.0
52	177387.5
53	185481.0
54	185481.0
55	171785.0
56	158089.0
57	149927.0
58	141765.0
59	130884.0
60	120003.0
61	120003.0
62	111586.0
63	103169.0
64	86867.0
65	70565.0
66	61884.5
67	53204.0
68	53204.0
69	45567.5
70	37931.0
71	32533.5
72	27136.0
73	22030.0
74	16924.0
75	16924.0
76	13641.0
77	10358.0
78	8559.0
79	6760.0
80	5164.5
81	3569.0
82	3569.0
83	2839.0
84	2109.0
85	1552.0
86	995.0
87	753.5
88	512.0
89	512.0
90	365.5
91	219.0
92	158.0
93	97.0
94	57.5
95	18.0
96	18.0
97	11.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1575821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.39483647056745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.78053262809016	38.03203578069193
2	8.67446742733311	7.8755206066610075
3	2.4883801951325104	3.3887883610391705
4	1.210801162894309	2.1985648315184023
5	0.7227140190038301	1.6403742353832722
6	0.4675772923275585	1.2735356833536138
7	0.3372500741233016	1.071658837515982
8	0.25954122597299234	0.9425465208331664
9	0.2022312966916274	0.826223097829251
>10	1.476712570509115	13.41945216459724
>50	0.19885698404691268	6.316823395851776
>100	0.16056590985846936	14.158939407523926
>500	0.01376677912810356	4.310368966639285
>1k	0.006602434887968034	4.545168110561967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3297	0.2092242710307833	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2735	0.17356032188935164	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2623	0.16645291565476028	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2343	0.14868440006828185	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2330	0.14785943327319537	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2209	0.1401808961804672	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2049	0.13002745870247953	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2024	0.12844098409654398	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2010	0.12755255831722004	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1951	0.1238084782472121	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1862	0.11816062865008146	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1823	0.11568572826482196	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1755	0.11137051733667719	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1686	0.10699184742429503	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1664	0.10559574977107172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	6.34589842374229E-5	0.0	0.0	0.0	0.0
9	6.34589842374229E-5	0.0	0.0	0.0	0.0
10	1.269179684748458E-4	0.0	0.0	0.0	0.0
11	1.269179684748458E-4	0.0	0.0	6.34589842374229E-5	0.0
12	1.269179684748458E-4	0.0	0.0	6.34589842374229E-5	0.0
13	1.269179684748458E-4	0.0	0.0	6.34589842374229E-5	0.0
14	1.269179684748458E-4	0.0	0.0	6.34589842374229E-5	6.34589842374229E-5
15	1.269179684748458E-4	0.0	0.0	1.269179684748458E-4	6.34589842374229E-5
16	2.538359369496916E-4	0.0	0.0	4.4421288966196035E-4	6.34589842374229E-5
17	2.538359369496916E-4	0.0	0.0	7.615078108490749E-4	6.34589842374229E-5
18	2.538359369496916E-4	0.0	0.0	0.0012057207005110352	6.34589842374229E-5
19	2.538359369496916E-4	0.0	0.0	0.0018403105428852643	6.34589842374229E-5
20	3.172949211871145E-4	0.0	0.0	0.002157605464072379	6.34589842374229E-5
21	3.8075390542453743E-4	0.0	0.0	0.0029191132749214537	6.34589842374229E-5
22	4.4421288966196035E-4	0.0	0.0	0.004949800770518987	6.34589842374229E-5
23	5.076718738993832E-4	0.0	0.0	0.006092062486792599	6.34589842374229E-5
24	5.076718738993832E-4	0.0	0.0	0.007741996076965595	6.34589842374229E-5
25	5.076718738993832E-4	0.0	0.0	0.009582306619850859	6.34589842374229E-5
26	5.076718738993832E-4	0.0	0.0	0.01307255075290912	6.34589842374229E-5
27	5.076718738993832E-4	0.0	0.0	0.03686966984194271	6.34589842374229E-5
28	5.076718738993832E-4	0.0	0.0	0.13669065204740893	6.34589842374229E-5
29	5.076718738993832E-4	0.0	0.0	0.27940990759737305	6.34589842374229E-5
30	5.076718738993832E-4	0.0	0.0	0.4784807411501687	6.34589842374229E-5
31	5.076718738993832E-4	0.0	0.0	1.0567190055215663	6.34589842374229E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1875	0.0	31.672	1
ATACGGC	190	0.0	30.184212	29
TACGGCT	255	0.0	22.490196	30
GTATCAA	2715	0.0	21.736649	2
GTTATCG	45	0.0038264291	20.555555	11
GCGGTAA	1335	0.0	19.816479	23
TAATACG	1315	0.0	18.851711	27
ACCATAC	60	9.23889E-4	18.5	8
TTTTACG	110	3.8542566E-8	18.5	4
CTTATAC	1730	0.0	18.393063	37
TATACAC	545	0.0	18.330275	37
CGCGGTA	1460	0.0	18.119864	22
CGTGCCA	1530	0.0	18.01634	10
AACTCCG	1540	0.0	17.899353	5
GGTAATA	1480	0.0	17.875	25
CGGTAAT	1480	0.0	17.875	24
AGCCGCG	1575	0.0	17.384127	19
GTCTATA	75	2.06788E-4	17.266666	2
CCGCGGT	1605	0.0	17.059189	21
TCTATGG	500	0.0	17.02	2
>>END_MODULE