FastQCFastQC Report
Fri 10 Feb 2017
ERR1632695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32835
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT1140.3471904979442668TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA940.28627988426983403No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA700.21318714786051468No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA610.18577737170701994No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT570.1735952489721334No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA540.16445865692096848No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA530.16141312623724682No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA490.1492310035023603No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT470.14313994213491701No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA440.1340033500837521No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT400.12182122734886554No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT380.11573016598142227No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA380.11573016598142227No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC370.11268463529770063No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC360.10963910461397898No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG360.10963910461397898No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC350.10659357393025734No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC350.10659357393025734No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC340.1035480432465357No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT330.10050251256281408No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA330.10050251256281408No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC330.10050251256281408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCCG358.686286E-426.4285725
GTGTCCC400.001891692823.12500210
CACGTGT400.001891692823.1250027
ACTCATC400.001891692823.12500222
GGATACC400.001891692823.1250021
TACTCAT400.001891692823.12500221
ATCGAGC400.001891692823.12500226
CTACTCA400.001891692823.12500220
ACGTGTC400.001891692823.1250028
ACAGCAT450.003748306520.55555735
AGCTCAC450.003748306520.55555730
CGCCCTA450.003748306520.55555716
TGTCCCG450.003748306520.55555711
CCGCCCT450.003748306520.55555715
TGCCAGC450.003748306520.55555712
CGAGCTC450.003748306520.55555728
TCATCGA450.003748306520.55555724
TACCACG450.003748306520.5555574
CACAGCA450.003748306520.55555734
CATCGAG450.003748306520.55555725
Produced by FastQC (version 0.11.5)