##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632695.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32835
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.982670930409625	34.0	33.0	34.0	31.0	34.0
2	33.12800365463682	34.0	33.0	34.0	31.0	34.0
3	33.197654941373536	34.0	33.0	34.0	31.0	34.0
4	36.51116186995584	37.0	37.0	37.0	35.0	37.0
5	36.48253388152886	37.0	37.0	37.0	35.0	37.0
6	36.52827775239835	37.0	37.0	37.0	35.0	37.0
7	36.51728338663012	37.0	37.0	37.0	35.0	37.0
8	36.48396528095021	37.0	37.0	37.0	35.0	37.0
9	38.26572255215471	39.0	39.0	39.0	37.0	39.0
10	38.31387239226435	39.0	39.0	39.0	37.0	39.0
11	38.37548347799604	39.0	39.0	39.0	37.0	39.0
12	38.34027714329222	39.0	39.0	39.0	37.0	39.0
13	38.36147403685092	39.0	39.0	39.0	37.0	39.0
14	39.90677630577128	41.0	40.0	41.0	38.0	41.0
15	39.91231917161566	41.0	40.0	41.0	38.0	41.0
16	39.85555047967108	41.0	40.0	41.0	38.0	41.0
17	39.86663621135983	41.0	40.0	41.0	38.0	41.0
18	39.85006852444038	41.0	40.0	41.0	38.0	41.0
19	39.85025125628141	41.0	40.0	41.0	38.0	41.0
20	39.84644434292675	41.0	40.0	41.0	38.0	41.0
21	39.78562509517283	41.0	40.0	41.0	38.0	41.0
22	39.76004263742957	41.0	40.0	41.0	38.0	41.0
23	39.71152733363789	41.0	40.0	41.0	38.0	41.0
24	39.68679762448607	41.0	40.0	41.0	37.0	41.0
25	39.64537840718745	41.0	40.0	41.0	37.0	41.0
26	39.5503273945485	41.0	40.0	41.0	37.0	41.0
27	39.43724684026191	41.0	39.0	41.0	37.0	41.0
28	39.42619156388	41.0	39.0	41.0	37.0	41.0
29	39.39537079336074	41.0	39.0	41.0	37.0	41.0
30	39.34371859296483	41.0	39.0	41.0	36.0	41.0
31	39.26779351301964	41.0	39.0	41.0	36.0	41.0
32	39.22960255824577	41.0	39.0	41.0	36.0	41.0
33	39.142378559463985	41.0	39.0	41.0	35.0	41.0
34	39.12739454850008	41.0	39.0	41.0	35.0	41.0
35	39.03484087102178	40.0	39.0	41.0	35.0	41.0
36	38.97770671539516	40.0	39.0	41.0	35.0	41.0
37	38.9050708085884	40.0	39.0	41.0	35.0	41.0
38	38.80733972894777	40.0	38.0	41.0	35.0	41.0
39	38.72657225521547	40.0	38.0	41.0	35.0	41.0
40	38.63998781787726	40.0	38.0	41.0	35.0	41.0
41	38.59424394700777	40.0	38.0	41.0	35.0	41.0
42	38.5223389675651	40.0	38.0	41.0	35.0	41.0
43	37.757058017359526	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	0.0
18	0.0
19	1.0
20	4.0
21	2.0
22	3.0
23	13.0
24	21.0
25	36.0
26	41.0
27	68.0
28	84.0
29	119.0
30	129.0
31	186.0
32	295.0
33	350.0
34	562.0
35	855.0
36	1421.0
37	2605.0
38	6969.0
39	19068.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.928734582000914	15.745393634840871	12.300898431551698	28.02497335160652
2	21.726815897670168	19.66803715547434	30.63194761687224	27.97319932998325
3	21.458809197502664	19.354347495051012	28.350845134764736	30.835998172681588
4	16.534186081924776	15.023602862798842	32.25826100197959	36.18395005329679
5	17.368661489264504	32.98918836607279	32.03898279275164	17.60316735191107
6	36.375818486371244	32.55063194761687	14.585046444342927	16.488503121668952
7	31.359829450281712	26.37125019034567	19.287345820009136	22.981574539363482
8	28.448302116643827	30.065478909700015	19.485305314451043	22.000913659205114
9	28.728490939546212	12.221714633774935	16.44586569209685	42.603928734582006
10	19.78985838282321	23.079031521242577	29.31018730013705	27.820922795797166
11	40.4781483173443	18.355413430790314	17.95340338053906	23.21303487132633
12	23.094259174661186	22.890208618851833	26.030150753768844	27.985381452718137
13	36.15044921577585	16.631643063803867	19.74112989188366	27.476777828536626
14	25.12867367138724	18.726968174204355	21.888229023907417	34.25612913050099
15	30.09593421653723	23.085122582610023	18.77265113446018	28.04629206639257
16	26.879853814527184	22.999847723465813	21.20603015075377	28.914268311253238
17	27.537688442211056	23.322673975940308	20.846657530074616	28.29298005177402
18	27.89706106289021	20.395918988883814	22.150144662707476	29.5568752855185
19	28.914268311253238	22.074006395614436	21.940003045530684	27.07172224760164
20	30.60453784071874	19.990863407948837	21.547129587330595	27.857469164001824
21	28.04324653570885	20.96847875742348	21.32176031673519	29.66651439013248
22	29.7121973503883	21.86386477843764	19.771585198720878	28.652352672453173
23	29.273640931932388	21.553220648698034	21.00197959494442	28.171158824425156
24	28.89904065783463	20.584741891274554	21.41312623724684	29.103091213643978
25	29.614740368509214	20.992843002893256	21.37657986904218	28.015836759555352
26	29.74265265722552	21.76640779655855	21.047662555200244	27.443276991015686
27	27.839195979899493	21.684178468098068	22.905436272270443	27.571189279731996
28	27.2910004568296	22.293284604842395	21.13293741434445	29.282777523983555
29	27.769148774173903	22.649611694837827	21.20907568143749	28.372163849550784
30	27.647327546825036	21.364397746307297	22.868889904065785	28.11938480280189
31	28.83813004416019	21.720724836302725	21.276077356479366	28.16506776305771
32	26.56007309273641	21.370488807674736	22.275011420740064	29.79442667884879
33	26.56007309273641	21.07811786203746	23.599817268158976	28.761991777067152
34	27.12045073854119	21.78468098066088	22.536927059540123	28.557941221257803
35	27.032130348713263	22.34201309578194	23.517587939698494	27.108268615806303
36	27.26663621135983	21.41312623724684	23.953098827470686	27.367138723922647
37	27.580325871783156	20.938023450586265	23.45667732602406	28.02497335160652
38	26.43520633470382	20.886249428962998	25.034262220191867	27.644282016141315
39	26.35602253692706	19.942134917009287	25.42713567839196	28.27470686767169
40	25.609867519415257	20.402010050251256	26.901172529313232	27.08694990102025
41	23.931780112684635	20.14923100350236	27.376275315973807	28.542713567839193
42	22.530835998172684	20.55428658443734	28.798538145271813	28.116339272118168
43	22.494289629968023	20.499467032130347	28.06151971981118	28.94472361809045
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	5.0
14	9.0
15	10.5
16	12.0
17	17.0
18	22.0
19	22.0
20	25.0
21	28.0
22	21.5
23	15.0
24	24.0
25	33.0
26	33.0
27	44.0
28	55.0
29	84.0
30	113.0
31	144.5
32	176.0
33	176.0
34	231.5
35	287.0
36	331.0
37	375.0
38	483.0
39	591.0
40	591.0
41	748.5
42	906.0
43	1041.5
44	1177.0
45	1641.0
46	2105.0
47	2105.0
48	2315.5
49	2526.0
50	2810.0
51	3094.0
52	3399.5
53	3705.0
54	3705.0
55	3490.0
56	3275.0
57	3249.5
58	3224.0
59	3076.0
60	2928.0
61	2928.0
62	2683.5
63	2439.0
64	2100.5
65	1762.0
66	1537.0
67	1312.0
68	1312.0
69	1142.5
70	973.0
71	810.0
72	647.0
73	530.5
74	414.0
75	414.0
76	337.0
77	260.0
78	217.5
79	175.0
80	125.5
81	76.0
82	76.0
83	70.0
84	64.0
85	44.0
86	24.0
87	20.5
88	17.0
89	17.0
90	12.5
91	8.0
92	5.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	32835.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.13933302878027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.92380025940338	67.56814374904828
2	5.309662775616083	7.979290391350694
3	1.7874513618677041	4.029237094563728
4	0.8025291828793774	2.4120603015075375
5	0.5147535667963683	1.9339119841632404
6	0.38099870298313876	1.717679305619004
7	0.1823929961089494	0.9593421653723162
8	0.19860570687418935	1.1938480280188823
9	0.12970168612191957	0.8771128369118318
>10	0.7417315175097275	9.797472209532511
>50	0.024319066147859923	1.1847114359677173
>100	0.0040531776913099865	0.3471904979442668
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	114	0.3471904979442668	TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	94	0.28627988426983403	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	70	0.21318714786051468	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	61	0.18577737170701994	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	57	0.1735952489721334	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	54	0.16445865692096848	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	53	0.16141312623724682	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	49	0.1492310035023603	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	47	0.14313994213491701	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	44	0.1340033500837521	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	40	0.12182122734886554	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	38	0.11573016598142227	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	38	0.11573016598142227	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	37	0.11268463529770063	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	36	0.10963910461397898	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	36	0.10963910461397898	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	35	0.10659357393025734	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	35	0.10659357393025734	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	34	0.1035480432465357	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	33	0.10050251256281408	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	33	0.10050251256281408	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	33	0.10050251256281408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.006091061367443277	0.0
17	0.0	0.0	0.0	0.006091061367443277	0.0
18	0.0	0.0	0.0	0.006091061367443277	0.0
19	0.0	0.0	0.0	0.006091061367443277	0.0
20	0.0	0.0	0.0	0.006091061367443277	0.0
21	0.0	0.0	0.0	0.015227653418608192	0.0
22	0.0	0.0	0.0	0.015227653418608192	0.0
23	0.0	0.0	0.0	0.01827318410232983	0.0
24	0.0	0.0	0.0	0.01827318410232983	0.0
25	0.0	0.0	0.0	0.01827318410232983	0.0
26	0.0	0.0	0.0	0.01827318410232983	0.0
27	0.0	0.0	0.0	0.04263742957210294	0.0
28	0.0	0.0	0.0	0.12791228871630883	0.0
29	0.0	0.0	0.0	0.2619156388000609	0.0
30	0.0	0.0	0.0	0.4263742957210294	0.0
31	0.0	0.0	0.0	0.8801583675955535	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCCG	35	8.686286E-4	26.428572	5
GTGTCCC	40	0.0018916928	23.125002	10
CACGTGT	40	0.0018916928	23.125002	7
ACTCATC	40	0.0018916928	23.125002	22
GGATACC	40	0.0018916928	23.125002	1
TACTCAT	40	0.0018916928	23.125002	21
ATCGAGC	40	0.0018916928	23.125002	26
CTACTCA	40	0.0018916928	23.125002	20
ACGTGTC	40	0.0018916928	23.125002	8
ACAGCAT	45	0.0037483065	20.555557	35
AGCTCAC	45	0.0037483065	20.555557	30
CGCCCTA	45	0.0037483065	20.555557	16
TGTCCCG	45	0.0037483065	20.555557	11
CCGCCCT	45	0.0037483065	20.555557	15
TGCCAGC	45	0.0037483065	20.555557	12
CGAGCTC	45	0.0037483065	20.555557	28
TCATCGA	45	0.0037483065	20.555557	24
TACCACG	45	0.0037483065	20.555557	4
CACAGCA	45	0.0037483065	20.555557	34
CATCGAG	45	0.0037483065	20.555557	25
>>END_MODULE