FastQCFastQC Report
Fri 10 Feb 2017
ERR1632694.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632694.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16880
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT530.31398104265402843TruSeq Adapter, Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA270.1599526066350711No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA200.11848341232227488No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA190.11255924170616113No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA190.11255924170616113No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA170.10071090047393365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251.1827749E-437.01
GTATCAA303.4524713E-430.8333322
AACGCAG450.003673871320.5555577
ACGCAGA450.003673871320.5555578
CAGAGTA450.003673871320.55555711
AGAGTAC450.003673871320.55555712
GCAGAGT450.003673871320.55555710
CGCAGAG450.003673871320.5555579
TATCAAC450.003673871320.5555573
CAACGCA450.003673871320.5555576
TCAACGC500.00675812318.55
GAGTACG500.00675812318.513
CTGTCTC2052.2341737E-711.73170737
CCTGTCT1250.00904777810.3636
Produced by FastQC (version 0.11.5)