##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632694.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16880
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.952251184834125	34.0	33.0	34.0	31.0	34.0
2	33.09691943127962	34.0	33.0	34.0	31.0	34.0
3	33.17511848341232	34.0	33.0	34.0	31.0	34.0
4	36.49988151658768	37.0	37.0	37.0	35.0	37.0
5	36.47529620853081	37.0	37.0	37.0	35.0	37.0
6	36.50420616113744	37.0	37.0	37.0	35.0	37.0
7	36.51486966824645	37.0	37.0	37.0	35.0	37.0
8	36.48696682464455	37.0	37.0	37.0	35.0	37.0
9	38.26007109004739	39.0	39.0	39.0	37.0	39.0
10	38.277488151658766	39.0	39.0	39.0	37.0	39.0
11	38.35248815165877	39.0	39.0	39.0	37.0	39.0
12	38.30663507109005	39.0	39.0	39.0	37.0	39.0
13	38.3571682464455	39.0	39.0	39.0	37.0	39.0
14	39.867772511848344	41.0	40.0	41.0	38.0	41.0
15	39.8561018957346	41.0	40.0	41.0	38.0	41.0
16	39.82191943127962	41.0	40.0	41.0	38.0	41.0
17	39.83613744075829	41.0	40.0	41.0	38.0	41.0
18	39.853436018957346	41.0	40.0	41.0	38.0	41.0
19	39.85989336492891	41.0	40.0	41.0	38.0	41.0
20	39.78471563981043	41.0	40.0	41.0	38.0	41.0
21	39.72802132701422	41.0	40.0	41.0	38.0	41.0
22	39.6997037914692	41.0	40.0	41.0	38.0	41.0
23	39.68998815165877	41.0	40.0	41.0	37.0	41.0
24	39.654561611374405	41.0	40.0	41.0	37.0	41.0
25	39.60462085308057	41.0	40.0	41.0	37.0	41.0
26	39.52067535545024	41.0	40.0	41.0	37.0	41.0
27	39.44010663507109	41.0	39.0	41.0	37.0	41.0
28	39.42754739336493	41.0	39.0	41.0	37.0	41.0
29	39.36901658767773	41.0	39.0	41.0	36.0	41.0
30	39.33904028436019	41.0	39.0	41.0	36.0	41.0
31	39.240165876777255	41.0	39.0	41.0	36.0	41.0
32	39.20876777251185	41.0	39.0	41.0	35.0	41.0
33	39.12375592417062	41.0	39.0	41.0	35.0	41.0
34	39.08015402843602	41.0	39.0	41.0	35.0	41.0
35	39.000296208530806	40.0	39.0	41.0	35.0	41.0
36	38.938803317535545	40.0	39.0	41.0	35.0	41.0
37	38.88986966824645	40.0	39.0	41.0	35.0	41.0
38	38.821267772511845	40.0	38.0	41.0	35.0	41.0
39	38.730450236966824	40.0	38.0	41.0	35.0	41.0
40	38.61640995260664	40.0	38.0	41.0	35.0	41.0
41	38.571800947867295	40.0	38.0	41.0	35.0	41.0
42	38.48163507109005	40.0	38.0	41.0	35.0	41.0
43	37.78412322274882	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	0.0
22	4.0
23	4.0
24	19.0
25	12.0
26	27.0
27	36.0
28	42.0
29	51.0
30	86.0
31	125.0
32	134.0
33	200.0
34	279.0
35	475.0
36	704.0
37	1367.0
38	3520.0
39	9792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09834123222749	16.007109004739338	12.162322274881516	28.732227488151658
2	21.658767772511847	18.803317535545023	31.226303317535546	28.31161137440758
3	21.20260663507109	19.31872037914692	27.553317535545023	31.925355450236964
4	16.226303317535546	14.77488151658768	31.001184834123226	37.997630331753555
5	17.932464454976305	32.731042654028435	31.854265402843602	17.482227488151658
6	37.38744075829384	31.70616113744076	14.206161137440759	16.700236966824644
7	32.22748815165877	26.321090047393366	18.584123222748815	22.86729857819905
8	28.773696682464454	30.396919431279624	18.767772511848342	22.061611374407583
9	27.778436018957347	12.588862559241706	16.492890995260666	43.139810426540286
10	19.745260663507107	23.252369668246445	29.170616113744074	27.83175355450237
11	40.568720379146924	19.40165876777251	17.754739336492893	22.274881516587676
12	24.27725118483412	22.94431279620853	25.20734597156398	27.571090047393366
13	35.00592417061611	16.74170616113744	20.088862559241708	28.163507109004737
14	25.177725118483412	17.422985781990523	21.59952606635071	35.79976303317536
15	29.46682464454976	23.767772511848342	19.41350710900474	27.35189573459716
16	27.29265402843602	22.642180094786728	21.25	28.81516587677725
17	28.31161137440758	22.630331753554504	21.0781990521327	27.979857819905213
18	27.037914691943126	21.06042654028436	22.15047393364929	29.751184834123222
19	29.218009478672986	21.563981042654028	21.504739336492893	27.713270142180097
20	30.177725118483412	20.657582938388625	21.81872037914692	27.345971563981042
21	28.116113744075825	21.504739336492893	21.481042654028435	28.89810426540284
22	29.08175355450237	22.091232227488153	20.385071090047393	28.441943127962084
23	29.46090047393365	21.25	21.18483412322275	28.104265402843602
24	29.06398104265403	20.906398104265403	21.32701421800948	28.70260663507109
25	28.850710900473935	21.238151658767773	21.81872037914692	28.092417061611375
26	29.377962085308056	21.700236966824647	20.99526066350711	27.92654028436019
27	27.707345971563978	21.842417061611375	22.363744075829384	28.086492890995263
28	27.446682464454973	22.144549763033176	21.404028436018958	29.00473933649289
29	27.470379146919434	22.393364928909953	21.362559241706162	28.773696682464454
30	27.736966824644547	21.475118483412324	22.209715639810426	28.578199052132703
31	28.329383886255926	21.71208530805687	21.34478672985782	28.613744075829384
32	26.404028436018955	21.54620853080569	22.6718009478673	29.377962085308056
33	27.174170616113745	21.46919431279621	23.25829383886256	28.098341232227487
34	27.191943127962087	21.232227488151658	22.885071090047393	28.690758293838865
35	26.528436018957347	21.77132701421801	23.726303317535542	27.9739336492891
36	27.3696682464455	20.835308056872037	24.117298578199055	27.677725118483416
37	26.830568720379144	19.70379146919431	23.850710900473935	29.61492890995261
38	25.888625592417064	20.100710900473935	25.468009478672986	28.542654028436022
39	25.876777251184834	18.915876777251185	26.7239336492891	28.48341232227488
40	25.00592417061611	19.8696682464455	27.82582938388626	27.29857819905213
41	24.312796208530806	19.28317535545024	27.902843601895732	28.501184834123222
42	22.21563981042654	20.278436018957347	28.96919431279621	28.536729857819903
43	21.321090047393366	20.313981042654028	29.040284360189574	29.324644549763036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	0.5
11	0.0
12	0.0
13	2.0
14	4.0
15	9.0
16	14.0
17	12.5
18	11.0
19	11.0
20	9.5
21	8.0
22	9.5
23	11.0
24	14.0
25	17.0
26	17.0
27	23.0
28	29.0
29	40.0
30	51.0
31	73.0
32	95.0
33	95.0
34	120.5
35	146.0
36	180.5
37	215.0
38	295.5
39	376.0
40	376.0
41	471.5
42	567.0
43	630.0
44	693.0
45	840.5
46	988.0
47	988.0
48	1106.5
49	1225.0
50	1408.0
51	1591.0
52	1701.5
53	1812.0
54	1812.0
55	1712.0
56	1612.0
57	1549.5
58	1487.0
59	1468.0
60	1449.0
61	1449.0
62	1350.5
63	1252.0
64	1089.5
65	927.0
66	839.5
67	752.0
68	752.0
69	643.0
70	534.0
71	459.5
72	385.0
73	313.0
74	241.0
75	241.0
76	188.5
77	136.0
78	123.5
79	111.0
80	85.5
81	60.0
82	60.0
83	45.5
84	31.0
85	26.0
86	21.0
87	17.0
88	13.0
89	13.0
90	8.5
91	4.0
92	5.5
93	7.0
94	3.5
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	16880.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.0781990521327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.67721954576737	79.77488151658768
2	4.397797660013764	7.5710900473933656
3	1.307639366827254	3.3767772511848344
4	0.5437026841018582	1.872037914691943
5	0.28217481073640743	1.2144549763033177
6	0.2202339986235375	1.1374407582938388
7	0.1445285615966965	0.8708530805687204
8	0.10323468685478321	0.7109004739336493
9	0.09635237439779766	0.7464454976303317
>10	0.2202339986235375	2.411137440758294
>50	0.006882312456985547	0.31398104265402843
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	53	0.31398104265402843	TruSeq Adapter, Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	27	0.1599526066350711	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	20	0.11848341232227488	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	19	0.11255924170616113	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	19	0.11255924170616113	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	17	0.10071090047393365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.005924170616113744	0.0
27	0.0	0.0	0.0	0.023696682464454975	0.0
28	0.0	0.0	0.0	0.1362559241706161	0.0
29	0.0	0.0	0.0	0.33767772511848343	0.0
30	0.0	0.0	0.0	0.4976303317535545	0.0
31	0.0	0.0	0.0	1.0959715639810426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25	1.1827749E-4	37.0	1
GTATCAA	30	3.4524713E-4	30.833332	2
AACGCAG	45	0.0036738713	20.555557	7
ACGCAGA	45	0.0036738713	20.555557	8
CAGAGTA	45	0.0036738713	20.555557	11
AGAGTAC	45	0.0036738713	20.555557	12
GCAGAGT	45	0.0036738713	20.555557	10
CGCAGAG	45	0.0036738713	20.555557	9
TATCAAC	45	0.0036738713	20.555557	3
CAACGCA	45	0.0036738713	20.555557	6
TCAACGC	50	0.006758123	18.5	5
GAGTACG	50	0.006758123	18.5	13
CTGTCTC	205	2.2341737E-7	11.731707	37
CCTGTCT	125	0.009047778	10.36	36
>>END_MODULE