FastQCFastQC Report
Fri 10 Feb 2017
ERR1632693.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632693.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279118
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA77480.6057298857493991No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA59960.46876050528567337No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA46200.36118637998996184No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA45770.3578246885744708No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA38720.3027085851344442No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA35980.28128757471945515No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC35400.2767532002520487No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG34480.2695607442003005No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA32870.2569739461097413No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT31850.24899970135671612No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT31190.24383989592828811No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA30590.23914916372062625No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT29900.2337548216818151No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC29740.23250395975977198No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT29610.2314876344481119No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC29560.2310967400974734No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT29440.23015859365594105No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA28760.2248424304872576No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT28440.22234070664317132No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC26800.20951937194222894No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC26790.20944119307210124No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA26570.20772125792929189No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC26350.20600132278648253No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA26300.2056104284358441No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC25250.19740164707243588No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT25200.19701075272179738No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC24870.19443085000758334No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA22810.1783260027612777No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA22810.1783260027612777No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC22720.1776223929301284No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG22430.17535520569642518No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG22060.1724625875017004No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC21240.16605192015122922No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG20980.16401926952790907No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC20820.16276840760586592No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC20670.16159572455395046No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA20370.15925035845011953No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA20250.15831221200858717No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC20090.157061350086544No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA19800.15479416285284078No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA19710.1540905530216915No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19540.15276151222952064No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC18860.14744534906083726No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA18710.14627266600892178No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA18590.1453345195673894No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT18150.1418946492817707No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC17950.14033107187921678No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA17720.13853295786627973No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT17620.13775116916500274No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC17460.1365003072429596No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG17360.13571851854168263No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC17230.13470219323002255No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT17110.1337640467884902No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC16500.12899513571070068No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA16280.12727520056789132No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC16260.12711884282763591No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC16200.12664976960686974No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC16110.12594615977572046No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15990.1250080133341881No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG15890.12422622463291112No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG15850.12391350915240032No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA15500.12117724869793092No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC14810.1157829066591198No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14550.11375025603579966No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA14550.11375025603579966No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG14420.11273393072413959No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA14060.10991949139954249No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC13980.10929406043852091No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT13550.10593236902302994No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13440.10507240145162526No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT13150.10280521421792205No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA13130.10264885647766665No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA13130.10264885647766665No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC13100.10241431986728355No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA13080.10225796212702816No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG12880.1006943847244742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCT1400.030.39285933
ATTACCT1600.028.9062533
GGTATCA24800.028.4959681
TATTGAC405.940647E-527.7530
TTTTGCT405.940647E-527.752
TCAATGC1550.027.45161432
GTATTGC754.620233E-1027.1333311
ATACGGC4300.026.67442129
ACAACTG358.869944E-426.42857235
CTAAGTC1500.025.89999834
AATTACC451.3233266E-424.66666632
TGGGAGT701.923272E-723.78571528
CTTTTTA1250.023.682
TTTTACG1250.023.684
ATACACA2350.022.82978837
AAACCGT652.6832404E-622.769236
TGTACGT652.6832404E-622.7692312
CCTAGTT1005.347829E-1022.22
CTAGTTC1005.347829E-1022.23
TACGGCT5250.022.19999930
Produced by FastQC (version 0.11.5)