FastQCFastQC Report
Fri 10 Feb 2017
ERR1632692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286036
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16630.5813953488372093No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12320.4307150148932302No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10740.3754772126585465No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9660.33771972758673735No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9080.31744255967780277No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8040.2810834999790236No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7670.2681480652784964No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7530.26325357647289155No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7360.25731026863751416No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7150.24996853542910685No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7130.24926932274259186No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT7020.24542365296675944No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT6930.24227719587744198No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6930.24227719587744198No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6630.23178900557971724No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6550.2289921548336573No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6350.22200002796850749No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6320.220951208938735No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC6210.21710553916290254No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6140.21465829476010012No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC6010.2101134122977527No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5650.19752758394048303No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5620.19647876491071053No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5400.18878742535904572No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5350.18703939364275826No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5140.17969766043435093No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5050.1765512033450335No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4920.17200632088268608No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4860.16990868282314114No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4710.16466458767427877No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4690.16396537498776378No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4510.15767246080912892No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4500.15732285446587144No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4470.15627403543609894No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4420.15452600371981148No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4390.15347718469003901No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4280.14963151491420662No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4240.14823308954117664No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4210.14718427051140415No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4050.1415905690192843No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3990.13949293095973933No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3950.13809450558670938No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3930.1373952929001944No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3870.13529765484064943No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3790.1325008040945895No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3730.13040316603504454No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3720.13005355969178703No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3710.12970395334852955No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3690.12900474066201456No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3690.12900474066201456No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3640.1272567089457271No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3630.12690710260246962No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3570.12480946454292467No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3500.12236222014012223No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3490.12201261379686473No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3430.11991497573731978No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3350.11711812499125986No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3200.11187402984239746No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3160.1104756044693675No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG3130.10942678543959501No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3130.10942678543959501No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3080.10767875372330755No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3080.10767875372330755No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3050.10662993469353509No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3030.1059307220070201No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC3020.1055811156637626No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3010.10523150932050512No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3010.10523150932050512No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2970.10383308394747515No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2960.10348347760421765No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2950.10313387126096017No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2930.10243465857444518No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2870.10033702051490022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA200.001839625237.034
TAAAGCC200.001839625237.030
CTAAGTC200.001839625237.034
GTTTCCA250.005489527729.613
TCCTCGA250.005489527729.62
TTTTTAC250.005489527729.63
ATGTTTG250.005489527729.633
TTTTACG250.005489527729.64
GCTTTTT250.005489527729.61
ATACACA250.005489527729.637
ATTGTTA453.9944316E-628.77777714
CTCCACT453.9944316E-628.7777772
GAGGCAG453.9944316E-628.77777717
GACCGTT803.274181E-1127.7500027
GGTATCA6200.027.4516141
TTAGGAC754.5838533E-1027.1333313
CTTAGGA754.5838533E-1027.1333312
ATACGGC1300.027.03846229
TTAGCCA358.853047E-426.428574
CCGCTTT358.853047E-426.4285726
Produced by FastQC (version 0.11.5)