FastQCFastQC Report
Fri 10 Feb 2017
ERR1632691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192395
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA33080.27742484663219824No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA28070.2354085684693411No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20710.1736840560384772No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA20200.16940694987818633No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18730.15707882035734802No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17490.1466795818499742No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16140.13535783024920434No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15650.13124845374225821No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15400.129151833075449No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14910.1250424565685029No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14880.12479086208848576No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14570.12219105246164233No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14390.12068148558153967No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14010.11749462216798963No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13900.11657210907459357No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC13850.11615278494123173No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13630.11430775875443959No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13450.11279819187433694No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13340.11187567878094089No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT13070.1096113284607869No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT13050.10944359880744217No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT12970.10877268019406322No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12610.10575354643385791No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC12460.10449557403377235No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC12250.10273441267365262No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA12200.10231508854029077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.028.6384181
GTACTAT358.86959E-426.4285721
ATACGGC2350.025.97872429
TATAGAC659.392534E-825.6153852
ATTAGAG901.4370016E-1024.6666663
GTATAGA751.3738827E-824.6666641
TATAATA806.960563E-720.81252
ATAATAC450.00382589120.5555553
ATATCTA450.00382589120.5555556
GATCTAG851.2453875E-619.58823621
GTATCAA26150.019.3135762
TATTAGA1254.129106E-1019.242
CGTATGC801.616753E-518.531
ATCTAGC902.1523538E-618.522
CCGCTTA902.1523538E-618.525
TCTAAGA609.23709E-418.52
TCGTATG701.21924575E-418.530
ATAGACC1002.8751492E-718.4999983
GCGGTAA14800.018.37523
TACGGCT3350.018.2238830
Produced by FastQC (version 0.11.5)