FastQCFastQC Report
Fri 10 Feb 2017
ERR1632690.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632690.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22686
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA870.38349642951600105No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA610.2688883011548973No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT470.20717623203737986TruSeq Adapter, Index 1 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT470.20717623203737986No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA460.20276822710041434No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA460.20276822710041434No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA450.1983602221634488No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA410.1807282024155867No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC370.1630961826677246No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT360.15868817773075905No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA350.15428017279379352No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC330.14546416291986247No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC320.14105615798289695No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC310.13664815304593142No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG310.13664815304593142No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC310.13664815304593142No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA310.13664815304593142No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC300.13224014810896587No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT300.13224014810896587No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA300.13224014810896587No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC290.12783214317200034No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT290.12783214317200034No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT290.12783214317200034No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT290.12783214317200034No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC280.12342413823503483No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC270.11901613329806929No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA260.11460812836110376No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA250.11020012342413824No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG240.10579211848717271No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA240.10579211848717271No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC230.10138411355020717No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC230.10138411355020717No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA230.10138411355020717No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA230.10138411355020717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGT303.4902472E-430.83333421
GCGGTAA358.602748E-426.4285723
CGCGGTA358.602748E-426.4285722
GCAGCCG400.001873671323.12500217
CGTGCCA400.001873671323.12500210
GCCGCGG400.001873671323.12500220
AGCCGCG400.001873671323.12500219
GTAATAC400.001873671323.12500226
CGGTAAT400.001873671323.12500224
GGTAATA400.001873671323.12500225
GTGCCAG400.001873671323.12500211
AACTCCG450.003712935620.5555575
GCTAACT450.003712935620.5555572
TGCCAGC450.003712935620.55555712
TCTTTAG450.003712935620.5555571
CTCCGTG450.003712935620.5555577
CAGCCGC450.003712935620.55555718
CTCTTAT554.932466E-420.18181837
CCGTGCC500.00682929418.59
CCAGCAG500.00682929418.514
Produced by FastQC (version 0.11.5)