FastQCFastQC Report
Fri 10 Feb 2017
ERR1632689.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632689.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690855
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA27690.4008076948129492No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20880.3022341880713029No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18230.26387592186493547No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16660.24115045848984232No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16250.2352157833409326No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16040.23217607167929596No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14380.2081478747349299No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14320.207279385688748No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT13280.1922255755549283No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT13200.19106759016001912No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12680.18354068509310928No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12570.1819484551751091No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12500.18093521795456355No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12350.17876399533910878No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC11560.16732888956438036No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11160.16153896258983433No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10620.1537225611741972No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC10460.1514065903843788No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10430.15097234586128783No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9940.14387968531746892No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC9820.1421427072251051No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9760.14127421817892322No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9680.140116232784014No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA9150.13244457954274053No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT9130.1321550831940132No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC9040.13085234962474038No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8830.1278126379631037No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC8360.1210094737680121No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC8320.1204304810705575No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8140.11782501393201178No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7960.11521954679346606No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7930.11478530227037512No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7910.1144958059216478No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7630.11044285703946559No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA7520.10885062712146544No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7360.106534656331647No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7350.10638990815728336No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA7200.1042186855418286No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7160.103639692844374No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT7070.10233695927510114No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6980.10103422570582829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC200.001841427437.03
GGTATCA13250.030.158491
TTTTACG604.3175532E-827.7500024
AGTCGGT1900.025.3157911
ATACGGC2200.024.38636429
CCGCTTA851.9736035E-923.94117725
GTAAGAG400.001930411123.1250023
CTCTATG2800.023.1250021
GCAGTCG2000.023.1249989
CAGTCGG2050.022.56097410
CTTATTG2150.022.37209328
GTATCAA18650.021.3270782
TCTATGG3050.021.229512
GCGGTAA11950.020.89958223
GACGCGG450.003824287320.5555536
CGCGGTA12150.020.40329222
GTGATTC2200.020.18181816
GATTCCT2300.020.10869618
AACGACC1950.019.92307737
TGCGGGT7250.019.90344821
Produced by FastQC (version 0.11.5)