##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632689.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690855
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00177750758119	34.0	33.0	34.0	31.0	34.0
2	33.14078352186783	34.0	33.0	34.0	31.0	34.0
3	33.215013280644996	34.0	33.0	34.0	31.0	34.0
4	36.50925736949143	37.0	37.0	37.0	35.0	37.0
5	36.483606545512444	37.0	37.0	37.0	35.0	37.0
6	36.53090880141274	37.0	37.0	37.0	35.0	37.0
7	36.540861685881985	37.0	37.0	37.0	35.0	37.0
8	36.510196785143044	37.0	37.0	37.0	35.0	37.0
9	38.312527230750305	39.0	39.0	39.0	37.0	39.0
10	38.3347055460263	39.0	39.0	39.0	37.0	39.0
11	38.40453496030281	39.0	39.0	39.0	37.0	39.0
12	38.355617314776616	39.0	39.0	39.0	37.0	39.0
13	38.39350080697107	39.0	39.0	39.0	37.0	39.0
14	39.91001295496161	41.0	40.0	41.0	38.0	41.0
15	39.91328571118397	41.0	40.0	41.0	38.0	41.0
16	39.863369303254665	41.0	40.0	41.0	38.0	41.0
17	39.877528569670915	41.0	40.0	41.0	38.0	41.0
18	39.89176600010132	41.0	40.0	41.0	38.0	41.0
19	39.89507639084902	41.0	40.0	41.0	38.0	41.0
20	39.87835218678305	41.0	40.0	41.0	38.0	41.0
21	39.83318207149112	41.0	40.0	41.0	38.0	41.0
22	39.804189012166084	41.0	40.0	41.0	38.0	41.0
23	39.76393454487555	41.0	40.0	41.0	38.0	41.0
24	39.74608275253129	41.0	40.0	41.0	38.0	41.0
25	39.704437255285114	41.0	40.0	41.0	38.0	41.0
26	39.618109444094635	41.0	40.0	41.0	37.0	41.0
27	39.50901564004024	41.0	39.0	41.0	37.0	41.0
28	39.477094325147824	41.0	39.0	41.0	37.0	41.0
29	39.425535025439494	41.0	39.0	41.0	37.0	41.0
30	39.39442140536003	41.0	39.0	41.0	36.0	41.0
31	39.3266647849404	41.0	39.0	41.0	36.0	41.0
32	39.27958688871037	41.0	39.0	41.0	36.0	41.0
33	39.21259019620615	41.0	39.0	41.0	35.0	41.0
34	39.183545027538344	41.0	39.0	41.0	35.0	41.0
35	39.09890642754268	41.0	39.0	41.0	35.0	41.0
36	39.04928096344385	40.0	39.0	41.0	35.0	41.0
37	38.99189265475389	40.0	39.0	41.0	35.0	41.0
38	38.924671602579416	40.0	39.0	41.0	35.0	41.0
39	38.81779678803801	40.0	38.0	41.0	35.0	41.0
40	38.74355689688864	40.0	38.0	41.0	35.0	41.0
41	38.68768989151124	40.0	38.0	41.0	35.0	41.0
42	38.619422310036114	40.0	38.0	41.0	35.0	41.0
43	37.88319980314248	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	12.0
20	36.0
21	67.0
22	137.0
23	255.0
24	380.0
25	577.0
26	764.0
27	1136.0
28	1625.0
29	2185.0
30	3107.0
31	3947.0
32	5414.0
33	7347.0
34	11417.0
35	17459.0
36	27601.0
37	53822.0
38	141979.0
39	411579.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.819983932952645	16.200215674779802	12.783869263448915	28.195931128818636
2	21.92370323729292	19.486867721880856	31.420341460943323	27.1690875798829
3	21.3822003169985	19.151341453705914	29.2569352469042	30.209522982391384
4	15.437248047708998	16.125091372285066	34.94191979503658	33.49574078496935
5	17.258614325726818	32.58064282664235	32.93426261661275	17.226480231018087
6	32.50305780518343	34.50000361870436	15.871926815323043	17.125011760789167
7	29.447568592541128	27.46697932272329	19.835710822097255	23.249741262638324
8	27.0985952189678	29.918289655571716	20.688711813622252	22.29440331183823
9	28.44750345586266	12.251340729965044	17.439260047332652	41.86189576683964
10	19.84179024542053	22.62269217129499	28.40813195243575	29.12738563084873
11	40.0975602695211	19.08417830080118	18.255784498918008	22.56247693075971
12	22.62037620050517	23.782269796122197	26.516273313502836	27.081080689869797
13	36.86793900311932	17.331567405606098	19.652893877875965	26.147599713398616
14	24.11301937454314	20.327854614933667	23.01684145008721	32.54228456043598
15	30.420421072439225	23.694986646980915	19.2800225807152	26.604569699864662
16	24.763228173784658	24.05439636392586	22.660181948455175	28.522193513834303
17	27.45453097972802	23.394199940653248	21.0942961981892	28.05697288142953
18	27.212222535843267	20.395741508710223	22.702593163543725	29.68944279190279
19	28.675192334136685	22.10290147715512	22.807680338131735	26.41422585057646
20	31.18671790752039	20.10754789355219	21.853645120900914	26.852089078026502
21	27.81886213460133	21.474260155893784	21.356145645613044	29.350732063891844
22	29.103502182078728	22.358526753081325	20.634575996410245	27.9033950684297
23	29.098870240499092	21.031764986864104	21.555898126234883	28.313466646401924
24	28.19679961786482	20.684803612914433	22.61357303631008	28.504823732910666
25	28.679679527541957	21.632180414124527	22.327261147418778	27.36087891091474
26	28.545208473558127	22.358092508558236	21.804864986140363	27.291834031743274
27	26.948925606675783	21.49828835283815	24.50239196358136	27.050394076904706
28	26.80794088484559	22.762953152253367	21.22674077773194	29.202365185169104
29	27.474071983267113	23.556896888637993	21.763322260097993	27.205708867996904
30	27.20440613442763	21.450231958949416	23.673129672652003	27.672232233970952
31	28.52002229121885	22.02922465640402	22.2061069254764	27.244646126900722
32	25.90471227681641	21.551410932829608	22.528895354307345	30.01498143604664
33	26.120097560269524	21.303457310144676	24.405989679455168	28.170455450130632
34	26.55434208336047	21.42938822184105	23.800363317917654	28.215906376880824
35	25.759095613406576	23.29113924050633	24.455493555087536	26.494271590999556
36	26.45359735400337	21.48960346237633	25.080371423815418	26.976427759804878
37	27.04576213532507	21.182592584551028	24.499931244617176	27.27171403550673
38	25.760108850627123	20.417308986690404	25.91035745561659	27.91222470706588
39	25.10628134702651	19.39770284647285	27.06255292355125	28.43346288294939
40	24.862235925049394	20.314972027415305	28.321717292340647	26.501074755194647
41	22.92883456007411	19.59716583074596	29.08019772600618	28.393801883173747
42	21.056082680157196	20.439744953716772	30.727287202090164	27.776885164035868
43	19.964391949106542	21.188672007874302	29.955200440034453	28.89173560298471
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	4.5
2	4.0
3	13.5
4	23.0
5	23.0
6	24.0
7	25.0
8	27.5
9	30.0
10	45.5
11	61.0
12	61.0
13	149.5
14	238.0
15	422.5
16	607.0
17	593.0
18	579.0
19	579.0
20	718.0
21	857.0
22	764.0
23	671.0
24	806.0
25	941.0
26	941.0
27	1168.0
28	1395.0
29	1791.5
30	2188.0
31	2678.5
32	3169.0
33	3169.0
34	4215.5
35	5262.0
36	6053.0
37	6844.0
38	9091.0
39	11338.0
40	11338.0
41	14341.5
42	17345.0
43	21535.0
44	25725.0
45	38435.0
46	51145.0
47	51145.0
48	58519.5
49	65894.0
50	74370.5
51	82847.0
52	84755.5
53	86664.0
54	86664.0
55	79290.5
56	71917.0
57	69047.5
58	66178.0
59	62323.0
60	58468.0
61	58468.0
62	52388.5
63	46309.0
64	37408.0
65	28507.0
66	24439.0
67	20371.0
68	20371.0
69	16946.5
70	13522.0
71	11302.0
72	9082.0
73	7018.0
74	4954.0
75	4954.0
76	3936.5
77	2919.0
78	2392.5
79	1866.0
80	1548.0
81	1230.0
82	1230.0
83	1029.5
84	829.0
85	609.5
86	390.0
87	331.5
88	273.0
89	273.0
90	191.0
91	109.0
92	82.5
93	56.0
94	34.0
95	12.0
96	12.0
97	9.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	690855.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.773835074495565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.91103827159839	38.46566658651222
2	7.057032885009057	6.319408530173742
3	2.0914728529234647	2.8092978173873977
4	1.0554217318377845	1.890211142213738
5	0.6573459271001773	1.4715949063437361
6	0.45399216564176326	1.2196182209734427
7	0.3448187866100316	1.0807201637585664
8	0.24902248943109087	0.8919753497302383
9	0.21002980626016887	0.8463455915598951
>10	1.5548830766291537	13.867985972276573
>50	0.22615476759519346	7.0684307765791345
>100	0.16636672384556042	15.019960636754798
>500	0.016246750375543	5.0441404033022605
>1k	0.006173765142706341	4.004643902434254
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2769	0.4008076948129492	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2088	0.3022341880713029	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1823	0.26387592186493547	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1666	0.24115045848984232	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1625	0.2352157833409326	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1604	0.23217607167929596	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1438	0.2081478747349299	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1432	0.207279385688748	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	1328	0.1922255755549283	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	1320	0.19106759016001912	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1268	0.18354068509310928	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1257	0.1819484551751091	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1250	0.18093521795456355	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1235	0.17876399533910878	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	1156	0.16732888956438036	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1116	0.16153896258983433	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1062	0.1537225611741972	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	1046	0.1514065903843788	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1043	0.15097234586128783	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	994	0.14387968531746892	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	982	0.1421427072251051	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	976	0.14127421817892322	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	968	0.140116232784014	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	915	0.13244457954274053	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	913	0.1321550831940132	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	904	0.13085234962474038	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	883	0.1278126379631037	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	836	0.1210094737680121	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	832	0.1204304810705575	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	814	0.11782501393201178	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	796	0.11521954679346606	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	793	0.11478530227037512	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	791	0.1144958059216478	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	763	0.11044285703946559	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	752	0.10885062712146544	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	736	0.106534656331647	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	735	0.10638990815728336	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	720	0.1042186855418286	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	716	0.103639692844374	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	707	0.10233695927510114	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	698	0.10103422570582829	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4474817436365084E-4
2	0.0	0.0	0.0	0.0	1.4474817436365084E-4
3	0.0	0.0	0.0	0.0	1.4474817436365084E-4
4	0.0	0.0	0.0	0.0	1.4474817436365084E-4
5	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
6	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
7	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
8	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
9	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
10	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
11	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
12	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
13	1.4474817436365084E-4	0.0	0.0	0.0	1.4474817436365084E-4
14	1.4474817436365084E-4	0.0	0.0	1.4474817436365084E-4	1.4474817436365084E-4
15	1.4474817436365084E-4	0.0	0.0	5.789926974546034E-4	1.4474817436365084E-4
16	1.4474817436365084E-4	0.0	0.0	0.001013237220545556	1.4474817436365084E-4
17	1.4474817436365084E-4	0.0	0.0	0.00173697809236381	1.4474817436365084E-4
18	1.4474817436365084E-4	0.0	0.0	0.002026474441091112	1.4474817436365084E-4
19	1.4474817436365084E-4	0.0	0.0	0.0023159707898184135	1.4474817436365084E-4
20	1.4474817436365084E-4	0.0	0.0	0.0033292080103639694	1.4474817436365084E-4
21	1.4474817436365084E-4	0.0	0.0	0.005645178800182383	1.4474817436365084E-4
22	1.4474817436365084E-4	0.0	0.0	0.009698127682364607	1.4474817436365084E-4
23	1.4474817436365084E-4	0.0	0.0	0.011290357600364766	1.4474817436365084E-4
24	2.894963487273017E-4	0.0	0.0	0.014330069262001434	1.4474817436365084E-4
25	2.894963487273017E-4	0.0	0.0	0.016501291877456196	1.4474817436365084E-4
26	2.894963487273017E-4	0.0	0.0	0.020409492585274767	1.4474817436365084E-4
27	2.894963487273017E-4	0.0	0.0	0.051819846422187	1.4474817436365084E-4
28	2.894963487273017E-4	0.0	0.0	0.1745662982825629	1.4474817436365084E-4
29	5.789926974546034E-4	0.0	0.0	0.3709895708940371	1.4474817436365084E-4
30	5.789926974546034E-4	0.0	0.0	0.6305230475280631	1.4474817436365084E-4
31	7.237408718182542E-4	0.0	0.0	1.297956879518857	1.4474817436365084E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGTAC	20	0.0018414274	37.0	3
GGTATCA	1325	0.0	30.15849	1
TTTTACG	60	4.3175532E-8	27.750002	4
AGTCGGT	190	0.0	25.31579	11
ATACGGC	220	0.0	24.386364	29
CCGCTTA	85	1.9736035E-9	23.941177	25
GTAAGAG	40	0.0019304111	23.125002	3
CTCTATG	280	0.0	23.125002	1
GCAGTCG	200	0.0	23.124998	9
CAGTCGG	205	0.0	22.560974	10
CTTATTG	215	0.0	22.372093	28
GTATCAA	1865	0.0	21.327078	2
TCTATGG	305	0.0	21.22951	2
GCGGTAA	1195	0.0	20.899582	23
GACGCGG	45	0.0038242873	20.555553	6
CGCGGTA	1215	0.0	20.403292	22
GTGATTC	220	0.0	20.181818	16
GATTCCT	230	0.0	20.108696	18
AACGACC	195	0.0	19.923077	37
TGCGGGT	725	0.0	19.903448	21
>>END_MODULE