FastQCFastQC Report
Fri 10 Feb 2017
ERR1632688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40662
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1430.35167970094928924No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA970.2385519649795878No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA920.22625547193940287No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT810.199203187250996No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT780.19182529142688506No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA760.18690669421081108No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA740.1819880969947371No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC680.16723230534651518No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT680.16723230534651518RNA PCR Primer, Index 46 (95% over 21bp)
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC660.1623137081304412No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA620.15247651369829326No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT590.14509861787418227No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA580.1426393192661453No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC570.1401800206581083No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC550.13526142344203435No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC510.12542422900988637No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA470.11558703457773842No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT470.11558703457773842No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC460.11312773596970144No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA450.11066843736166444No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA440.10820913875362746No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA440.10820913875362746No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC430.10574984014559048No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG430.10574984014559048No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC430.10574984014559048No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC420.10329054153755349No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC420.10329054153755349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAA401.5504047E-632.37531
ACATCTA401.5504047E-632.37530
GGTATCA701.9281288E-1029.0714281
GAAACAT453.8847047E-628.77777727
AAACATC453.8847047E-628.77777728
ATCTAAG453.8847047E-628.77777732
GAACTGA453.8847047E-628.77777722
GCGGTAA556.0158527E-726.9090923
ACTGAAA508.812804E-625.89999824
CTGAAAC508.812804E-625.89999825
TCTAAGT508.812804E-625.89999833
TGAAACA508.812804E-625.89999826
AACATCT508.812804E-625.89999829
CGCGGTA601.2852088E-624.66666622
AGCCGCG601.2852088E-624.66666619
GGTAATA601.2852088E-624.66666625
CCGCGGT601.2852088E-624.66666621
ATACGGA551.8448212E-523.54545429
GGGAACT551.8448212E-523.54545420
CCAGCAG652.5787776E-622.7692314
Produced by FastQC (version 0.11.5)