##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632688.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40662
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89002016624858	34.0	33.0	34.0	31.0	34.0
2	33.04731690521863	34.0	33.0	34.0	31.0	34.0
3	33.12156313019527	34.0	33.0	34.0	31.0	34.0
4	36.45959372386995	37.0	37.0	37.0	35.0	37.0
5	36.42058924794649	37.0	37.0	37.0	35.0	37.0
6	36.456962274359356	37.0	37.0	37.0	35.0	37.0
7	36.45824110963553	37.0	37.0	37.0	35.0	37.0
8	36.44464118833309	37.0	37.0	37.0	35.0	37.0
9	38.22291082583247	39.0	39.0	39.0	37.0	39.0
10	38.2407899267129	39.0	39.0	39.0	37.0	39.0
11	38.31260144606758	39.0	39.0	39.0	37.0	39.0
12	38.250504156214646	39.0	39.0	39.0	37.0	39.0
13	38.304338202744574	39.0	39.0	39.0	37.0	39.0
14	39.80817470857311	41.0	40.0	41.0	38.0	41.0
15	39.79004967783188	41.0	40.0	41.0	38.0	41.0
16	39.7424130637942	41.0	40.0	41.0	38.0	41.0
17	39.75242240912892	41.0	40.0	41.0	38.0	41.0
18	39.75827553981605	41.0	40.0	41.0	38.0	41.0
19	39.75726722738675	41.0	40.0	41.0	38.0	41.0
20	39.719074320003934	41.0	40.0	41.0	38.0	41.0
21	39.6770203138065	41.0	40.0	41.0	38.0	41.0
22	39.655968717721706	41.0	40.0	41.0	37.0	41.0
23	39.60356106438444	41.0	40.0	41.0	37.0	41.0
24	39.571909891299	41.0	40.0	41.0	37.0	41.0
25	39.533003787319856	41.0	39.0	41.0	37.0	41.0
26	39.42954109487974	41.0	39.0	41.0	37.0	41.0
27	39.32157788598692	41.0	39.0	41.0	36.0	41.0
28	39.278884462151396	41.0	39.0	41.0	36.0	41.0
29	39.21538537209188	41.0	39.0	41.0	36.0	41.0
30	39.187890413654024	40.0	39.0	41.0	36.0	41.0
31	39.14485268801338	40.0	39.0	41.0	35.0	41.0
32	39.070385126162016	40.0	39.0	41.0	35.0	41.0
33	39.01239486498451	40.0	39.0	41.0	35.0	41.0
34	38.9359844572328	40.0	39.0	41.0	35.0	41.0
35	38.841916285475385	40.0	38.0	41.0	35.0	41.0
36	38.75237322315675	40.0	38.0	41.0	35.0	41.0
37	38.701883822733755	40.0	38.0	41.0	35.0	41.0
38	38.60961093896021	40.0	38.0	41.0	35.0	41.0
39	38.52097781712656	40.0	38.0	41.0	35.0	41.0
40	38.44016526486646	40.0	38.0	41.0	35.0	41.0
41	38.38082238945453	40.0	37.0	41.0	35.0	41.0
42	38.31164231961045	40.0	37.0	41.0	35.0	41.0
43	37.5476120210516	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	3.0
20	2.0
21	4.0
22	10.0
23	24.0
24	36.0
25	36.0
26	56.0
27	77.0
28	128.0
29	133.0
30	212.0
31	257.0
32	391.0
33	490.0
34	725.0
35	1205.0
36	1937.0
37	3819.0
38	9462.0
39	21654.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.406620431852836	16.292853278245044	12.901480497762039	27.399045792140083
2	22.173036250061482	19.89080714180316	30.866656829472234	27.069499778663126
3	22.032856229403375	18.87511681668388	29.044316560916826	30.047710392995917
4	15.653435640155427	16.056760611873493	33.72436181201122	34.56544193595986
5	17.532339776695686	32.82179922286164	32.31518370960602	17.330677290836654
6	33.84240814519699	34.21868083222665	15.188628203236437	16.750282819339922
7	30.008361615267326	27.175249618808717	19.059564212286656	23.7568245536373
8	27.219516993753384	29.971472136146772	20.707294279671437	22.10171659042841
9	27.782696374993854	12.362894102601937	16.78717229846048	43.067237223943735
10	20.488416703556144	22.46077418720181	27.94992868034037	29.100880428901675
11	40.421031921695935	18.939058580492844	18.06108897742364	22.578820520387584
12	22.86163986031184	23.176430082140573	26.14726280064926	27.814667256898336
13	37.135408981358516	16.644532979194334	19.863754857114753	26.356303182332397
14	24.36427130982244	19.443214795140427	22.91082583247258	33.281688062564555
15	30.5321922187792	23.129703408587872	18.99316314986966	27.344941222763268
16	25.893955044021443	23.6904234912203	21.786926368599676	28.628695096158573
17	27.43101667404456	22.937878117160988	21.38360139688161	28.247503811912843
18	27.63759775711967	20.19084157198367	22.46077418720181	29.71078648369485
19	29.2287639565196	21.971373764202447	22.084501500172152	26.7153607791058
20	30.82238945452757	20.171167183119373	21.28522945256013	27.721213909792926
21	28.899217943042643	21.31965963307265	20.719590772711623	29.061531651173084
22	28.91151443608283	22.41158821504107	20.153952092863115	28.52294525601298
23	29.7673503516797	20.97289852933943	20.803206925384877	28.456544193595985
24	28.613939304510357	20.503172495204367	21.730362500614824	29.152525699670456
25	29.496827504795633	21.841030937976488	21.236043480399392	27.426098076828488
26	28.931188824947125	22.177954847277555	21.51394422310757	27.37691210466775
27	28.149131867591365	21.18685750823865	23.198563769612907	27.46544685455708
28	27.310511042250752	23.166592887708426	20.99995081402784	28.52294525601298
29	27.713836013968816	23.673208400964047	20.985195022379617	27.62776056268752
30	27.854016034626923	21.813978653288082	23.210860262653092	27.1211450494319
31	28.933648123555162	21.540996507795978	21.555752299444197	27.969603069204663
32	25.566868329152527	21.568048792484383	23.06576164477891	29.79932123358418
33	26.540750577935174	21.425409473218238	23.77403964389356	28.259800304953025
34	26.671093404161134	21.3098224386405	23.761743150853377	28.257341006344987
35	26.250553342186812	22.67227386749299	24.602823274802027	26.474349515518174
36	26.36368107815651	21.287688751168165	25.2791303920122	27.069499778663126
37	26.506320397422655	20.761398849048252	24.578230288721656	28.15405046480744
38	24.996311052087943	19.994097683340712	26.452215828045837	28.5573754365255
39	24.686439427475285	18.936599281884806	27.620382666863406	28.756578623776498
40	24.391323594510844	19.738330628104865	29.07382814421327	26.796517633171018
41	22.775564409030544	19.106290885839357	30.082140573508436	28.03600413162166
42	21.181938911022577	19.551423933894053	31.363435148295704	27.903202006787666
43	19.86621415572279	20.230190349712263	30.812552260095423	29.091043234469527
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.0
7	0.0
8	2.0
9	4.0
10	7.5
11	11.0
12	11.0
13	15.0
14	19.0
15	29.5
16	40.0
17	39.5
18	39.0
19	39.0
20	51.0
21	63.0
22	52.5
23	42.0
24	61.0
25	80.0
26	80.0
27	95.5
28	111.0
29	131.5
30	152.0
31	202.0
32	252.0
33	252.0
34	304.5
35	357.0
36	404.0
37	451.0
38	574.0
39	697.0
40	697.0
41	836.5
42	976.0
43	1228.5
44	1481.0
45	2116.5
46	2752.0
47	2752.0
48	3212.5
49	3673.0
50	3954.0
51	4235.0
52	4375.0
53	4515.0
54	4515.0
55	4383.0
56	4251.0
57	4086.5
58	3922.0
59	3732.5
60	3543.0
61	3543.0
62	3221.0
63	2899.0
64	2411.5
65	1924.0
66	1675.0
67	1426.0
68	1426.0
69	1237.0
70	1048.0
71	905.0
72	762.0
73	581.0
74	400.0
75	400.0
76	325.0
77	250.0
78	196.5
79	143.0
80	103.0
81	63.0
82	63.0
83	56.5
84	50.0
85	35.5
86	21.0
87	13.0
88	5.0
89	5.0
90	3.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	40662.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.32364369681767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8094072390278	63.15724755299789
2	4.916943521594684	6.91554768580001
3	1.7135862913096693	3.6151689538143725
4	0.9022556390977444	2.5379961634941712
5	0.6084979891589438	2.139589788992179
6	0.2902605350585767	1.22473070680242
7	0.2867634201783529	1.411637401013231
8	0.19933554817275745	1.1214401652648665
9	0.15387305472984789	0.9738822487826472
>10	1.06312292358804	13.988490482514388
>50	0.05245672320335724	2.5625891495745416
>100	0.0034971148802238152	0.35167970094928924
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	143	0.35167970094928924	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	97	0.2385519649795878	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	92	0.22625547193940287	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	81	0.199203187250996	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	78	0.19182529142688506	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	76	0.18690669421081108	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	74	0.1819880969947371	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	68	0.16723230534651518	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	68	0.16723230534651518	RNA PCR Primer, Index 46 (95% over 21bp)
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	66	0.1623137081304412	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	62	0.15247651369829326	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	59	0.14509861787418227	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	58	0.1426393192661453	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	57	0.1401800206581083	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	55	0.13526142344203435	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	51	0.12542422900988637	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	47	0.11558703457773842	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	47	0.11558703457773842	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	46	0.11312773596970144	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	45	0.11066843736166444	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	44	0.10820913875362746	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	44	0.10820913875362746	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	43	0.10574984014559048	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	43	0.10574984014559048	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	43	0.10574984014559048	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	42	0.10329054153755349	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	42	0.10329054153755349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.004918597216073976	0.0
22	0.0	0.0	0.0	0.007377895824110963	0.0
23	0.002459298608036988	0.0	0.0	0.007377895824110963	0.0
24	0.002459298608036988	0.0	0.0	0.009837194432147952	0.0
25	0.002459298608036988	0.0	0.0	0.009837194432147952	0.0
26	0.002459298608036988	0.0	0.0	0.012296493040184939	0.0
27	0.002459298608036988	0.0	0.0	0.06886036102503566	0.0
28	0.002459298608036988	0.0	0.0	0.2213368747233289	0.0
29	0.002459298608036988	0.0	0.0	0.5066155132556195	0.0
30	0.002459298608036988	0.0	0.0	0.8361615267325758	0.0
31	0.002459298608036988	0.0	0.0	1.6747823520731888	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCTAA	40	1.5504047E-6	32.375	31
ACATCTA	40	1.5504047E-6	32.375	30
GGTATCA	70	1.9281288E-10	29.071428	1
GAAACAT	45	3.8847047E-6	28.777777	27
AAACATC	45	3.8847047E-6	28.777777	28
ATCTAAG	45	3.8847047E-6	28.777777	32
GAACTGA	45	3.8847047E-6	28.777777	22
GCGGTAA	55	6.0158527E-7	26.90909	23
ACTGAAA	50	8.812804E-6	25.899998	24
CTGAAAC	50	8.812804E-6	25.899998	25
TCTAAGT	50	8.812804E-6	25.899998	33
TGAAACA	50	8.812804E-6	25.899998	26
AACATCT	50	8.812804E-6	25.899998	29
CGCGGTA	60	1.2852088E-6	24.666666	22
AGCCGCG	60	1.2852088E-6	24.666666	19
GGTAATA	60	1.2852088E-6	24.666666	25
CCGCGGT	60	1.2852088E-6	24.666666	21
ATACGGA	55	1.8448212E-5	23.545454	29
GGGAACT	55	1.8448212E-5	23.545454	20
CCAGCAG	65	2.5787776E-6	22.76923	14
>>END_MODULE