FastQCFastQC Report
Fri 10 Feb 2017
ERR1632686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159239
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7340.4609423570858898No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5090.3196453130200516No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4540.28510603558173564No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4170.2618705216686867No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3600.226075270505341No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3520.22105137560522234No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3520.22105137560522234No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3240.20346774345480692No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3200.20095579600474758No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3060.19216397992953987No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3010.1890240456169657No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2950.18525612444187667No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2910.18274417699181733No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2910.18274417699181733No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2840.1783482689542135No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2740.17206840032906512No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2550.16013664994128324No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2520.1582526893537387No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2520.1582526893537387No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2450.15385678131613487No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2440.15322879445362003No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2430.1526008075911052No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2410.15134483386607553No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2340.1469489258284717No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2260.14192503092835296No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2260.14192503092835296No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2060.12936529367805627No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2040.12810931995302657No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT1990.1249693856404524Illumina PCR Primer Index 10 (95% over 22bp)
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1960.1230854250529079No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1940.12182945132787823No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1910.11994549074033371No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1880.1180615301527892No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1840.11554958270272986No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1840.11554958270272986No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1820.11429360897770018No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1810.11366562211518536No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1780.11178166152764084No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1720.10801374035255182No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1710.107385753490037No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1690.1061297797650073No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC1680.10550179290249248No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1600.10047789800237379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC200.001837177437.04
GGTATCA2450.030.204081
GCAAGTC250.005482306229.613
GATTTTG250.005482306229.617
ATACGGC405.908529E-527.75000229
AGGACCG358.8357343E-426.428575
ATGCCCC358.8357343E-426.4285737
GCGGTAA2350.023.61702223
CGCGGTA2400.023.12499822
AGCCGCG2500.022.94000219
CCGCGGT2450.022.6530621
GGTAATA2500.022.225
TACGGCT502.6879588E-422.230
CGGTAAT2550.021.76470624
TATACAC855.1104507E-821.76470637
CAGCCGC2600.021.34615318
GCAGCCG2600.021.34615317
TAATACG2450.021.14285727
CAGTCGG705.056516E-621.14285710
CGTGCCA2650.020.94339610
Produced by FastQC (version 0.11.5)