##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632686.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	159239
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91937276672172	34.0	33.0	34.0	31.0	34.0
2	33.06119731975207	34.0	33.0	34.0	31.0	34.0
3	33.142358341863485	34.0	33.0	34.0	31.0	34.0
4	36.459874779419614	37.0	37.0	37.0	35.0	37.0
5	36.42767161310985	37.0	37.0	37.0	35.0	37.0
6	36.46889895063395	37.0	37.0	37.0	35.0	37.0
7	36.47843807107555	37.0	37.0	37.0	35.0	37.0
8	36.45233265720081	37.0	37.0	37.0	35.0	37.0
9	38.23227350083836	39.0	39.0	39.0	37.0	39.0
10	38.242616444463984	39.0	39.0	39.0	37.0	39.0
11	38.312128310275746	39.0	39.0	39.0	37.0	39.0
12	38.27215694647668	39.0	39.0	39.0	37.0	39.0
13	38.31417554744755	39.0	39.0	39.0	37.0	39.0
14	39.802473012264585	41.0	40.0	41.0	38.0	41.0
15	39.809380867752246	41.0	40.0	41.0	38.0	41.0
16	39.75657345248337	41.0	40.0	41.0	38.0	41.0
17	39.7651957121057	41.0	40.0	41.0	38.0	41.0
18	39.77912446071628	41.0	40.0	41.0	38.0	41.0
19	39.77219776562274	41.0	40.0	41.0	38.0	41.0
20	39.7575028730399	41.0	40.0	41.0	38.0	41.0
21	39.71206802353695	41.0	40.0	41.0	38.0	41.0
22	39.677239872141875	41.0	40.0	41.0	38.0	41.0
23	39.64420148330497	41.0	40.0	41.0	37.0	41.0
24	39.62754099184245	41.0	40.0	41.0	37.0	41.0
25	39.589585465872055	41.0	40.0	41.0	37.0	41.0
26	39.49465897173431	41.0	39.0	41.0	37.0	41.0
27	39.35942200089174	41.0	39.0	41.0	36.0	41.0
28	39.332092012635094	41.0	39.0	41.0	36.0	41.0
29	39.26714561131381	41.0	39.0	41.0	36.0	41.0
30	39.263440488824976	41.0	39.0	41.0	36.0	41.0
31	39.19366486853095	40.0	39.0	41.0	36.0	41.0
32	39.14454373614504	40.0	39.0	41.0	35.0	41.0
33	39.06941138791377	40.0	39.0	41.0	35.0	41.0
34	39.04400931932504	40.0	39.0	41.0	35.0	41.0
35	38.954596549840176	40.0	39.0	41.0	35.0	41.0
36	38.912132078196926	40.0	39.0	41.0	35.0	41.0
37	38.84336751675155	40.0	38.0	41.0	35.0	41.0
38	38.768574281426034	40.0	38.0	41.0	35.0	41.0
39	38.649608450191224	40.0	38.0	41.0	35.0	41.0
40	38.58280948762552	40.0	38.0	41.0	35.0	41.0
41	38.521794284063574	40.0	38.0	41.0	35.0	41.0
42	38.46609184935851	40.0	38.0	41.0	35.0	41.0
43	37.71758174818983	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	7.0
21	25.0
22	38.0
23	64.0
24	108.0
25	146.0
26	215.0
27	296.0
28	391.0
29	569.0
30	747.0
31	1049.0
32	1350.0
33	1834.0
34	2703.0
35	4350.0
36	7109.0
37	13737.0
38	38038.0
39	86456.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.846915642524756	16.04820427156664	12.80716407412757	28.29771601178103
2	22.12648911384774	19.482664422660278	31.29949321460195	27.091353248890034
3	21.750952970063867	19.295524337630855	28.68957981399029	30.263942878314985
4	15.712859286983715	16.24727610698384	34.47019888343936	33.569665722593086
5	17.618171427853728	32.50648396435547	32.35388315676436	17.521461451026443
6	32.36895484146471	34.26421919253449	16.104095102330458	17.26273086367033
7	29.362781730606198	27.189319199442348	19.51720370009859	23.930695369852863
8	26.39365984463605	30.055451239960064	20.908194600568955	22.642694314834934
9	27.830493786069994	12.454235457394232	17.515181582401297	42.200089174134476
10	19.848780763506426	22.27092609222615	28.273852511005472	29.606440633261954
11	39.982039575732074	19.26600895509266	18.13249266825338	22.619458800921883
12	22.33560873906518	23.564579029006712	26.424431200899278	27.675381031028827
13	37.02045353211211	16.938689642612676	19.75332676040417	26.287530064871046
14	24.31062742167434	20.137026733400738	22.46936994078084	33.082975904144085
15	30.431615370606448	23.495500474130083	19.12471191102682	26.948172244236652
16	24.955569929477075	23.92629946181526	22.52777271899472	28.590357889712948
17	27.637701819277943	23.24493371598666	20.95529361525757	28.16207084947783
18	26.93247257267378	20.42841263760762	22.748196107737424	29.89091868198117
19	28.64938865478934	22.14470073286067	22.797807069876097	26.408103542473892
20	31.295725293426862	19.93732691112102	21.68250240204975	27.08444539340237
21	28.249989010229903	21.188904728113087	21.292522560428036	29.268583701228973
22	28.776869987879856	22.613178932296737	20.329818700192792	28.28013237963062
23	29.135450486375824	21.12422208127406	21.47087082938225	28.269456602967868
24	28.26506069493026	20.68400329065116	22.40719924139187	28.643736773026706
25	29.387901205106786	21.67496655969957	21.750324983201352	27.18680725199229
26	28.906235281557908	22.142816772273125	21.78926016867727	27.161687777491693
27	27.21067075276785	21.46145102644453	24.282368012861173	27.045510207926448
28	26.737168658431663	22.988087089218094	21.309478205715937	28.9652660466343
29	27.39592687720973	23.64056543937101	21.470242842519735	27.493264840899528
30	27.337524098995846	21.57323268797217	23.842777209100785	27.246466003931197
31	28.916911058220663	21.70699388968783	22.059922506421163	27.31617254567034
32	25.867406853848617	21.384836629217716	22.74631214714988	30.001444369783787
33	26.32520927662193	21.468986868794705	24.10276377018193	28.10304008440143
34	26.65364640571719	21.317014048066117	23.43960964336626	28.58972990285043
35	25.93334547441268	23.02137039293138	24.1649344695709	26.880349663085045
36	26.612199272791216	21.102870527948557	25.163433580969485	27.121496618290745
37	27.282889241957058	20.48053554719635	24.544238534529857	27.69233667631673
38	25.756881166046007	19.836221026256133	26.36728439641043	28.039613411287434
39	25.20174077958289	18.932547931097282	27.5554355402885	28.310275749031334
40	24.94866207398941	19.922255226420663	28.758658368866925	26.370424330723
41	22.994366957843244	19.21200208491638	29.516010525059816	28.277620432180562
42	20.875539283718183	20.255716250416043	30.947192584731127	27.921551881134647
43	19.855060632131575	21.26489113847738	30.039123581534678	28.840924647856365
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	5.0
6	6.0
7	7.0
8	5.5
9	4.0
10	8.0
11	12.0
12	12.0
13	27.5
14	43.0
15	68.0
16	93.0
17	92.5
18	92.0
19	92.0
20	116.0
21	140.0
22	114.0
23	88.0
24	124.0
25	160.0
26	160.0
27	198.5
28	237.0
29	316.0
30	395.0
31	504.0
32	613.0
33	613.0
34	846.5
35	1080.0
36	1266.0
37	1452.0
38	1989.5
39	2527.0
40	2527.0
41	3211.5
42	3896.0
43	4857.5
44	5819.0
45	8687.5
46	11556.0
47	11556.0
48	13300.5
49	15045.0
50	17006.0
51	18967.0
52	19621.5
53	20276.0
54	20276.0
55	18636.0
56	16996.0
57	16143.5
58	15291.0
59	14603.5
60	13916.0
61	13916.0
62	12609.0
63	11302.0
64	8972.0
65	6642.0
66	5650.0
67	4658.0
68	4658.0
69	3918.5
70	3179.0
71	2675.0
72	2171.0
73	1633.0
74	1095.0
75	1095.0
76	866.5
77	638.0
78	525.0
79	412.0
80	322.0
81	232.0
82	232.0
83	171.5
84	111.0
85	77.5
86	44.0
87	36.5
88	29.0
89	29.0
90	20.0
91	11.0
92	8.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	159239.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.248123889248234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.56128763463919	43.84855468823592
2	6.452877816853947	6.613957636006254
3	2.297597019863492	3.532426101645954
4	1.2878797162008162	2.640056770012371
5	0.8001764554511255	2.05037710611094
6	0.548972514612717	1.6880286864398797
7	0.3761932187189824	1.349543767544383
8	0.32595243055130074	1.3363560434315713
9	0.26713394684279534	1.2321102242541087
>10	1.7731322067959858	17.653966679017074
>50	0.1887093018981215	6.698107875583243
>100	0.1176369674170108	10.575926751612357
>500	0.0024507701545210584	0.7805876701059413
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	734	0.4609423570858898	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	509	0.3196453130200516	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	454	0.28510603558173564	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	417	0.2618705216686867	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	360	0.226075270505341	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	352	0.22105137560522234	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	352	0.22105137560522234	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	324	0.20346774345480692	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	320	0.20095579600474758	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	306	0.19216397992953987	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	301	0.1890240456169657	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	295	0.18525612444187667	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	291	0.18274417699181733	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	291	0.18274417699181733	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	284	0.1783482689542135	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	274	0.17206840032906512	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	255	0.16013664994128324	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	252	0.1582526893537387	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	252	0.1582526893537387	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	245	0.15385678131613487	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	244	0.15322879445362003	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	243	0.1526008075911052	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	241	0.15134483386607553	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	234	0.1469489258284717	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	226	0.14192503092835296	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	226	0.14192503092835296	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	206	0.12936529367805627	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	204	0.12810931995302657	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	199	0.1249693856404524	Illumina PCR Primer Index 10 (95% over 22bp)
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	196	0.1230854250529079	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	194	0.12182945132787823	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	191	0.11994549074033371	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	188	0.1180615301527892	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	184	0.11554958270272986	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	184	0.11554958270272986	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	182	0.11429360897770018	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	181	0.11366562211518536	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	178	0.11178166152764084	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	172	0.10801374035255182	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	171	0.107385753490037	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	169	0.1061297797650073	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	168	0.10550179290249248	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	160	0.10047789800237379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	6.279868625148362E-4	0.0
20	0.0	0.0	0.0	6.279868625148362E-4	0.0
21	0.0	0.0	0.0	0.0012559737250296724	0.0
22	0.0	0.0	0.0	0.002511947450059345	0.0
23	0.0	0.0	0.0	0.003139934312574181	0.0
24	0.0	0.0	0.0	0.004395908037603854	0.0
25	0.0	0.0	0.0	0.005651881762633526	0.0
26	0.0	0.0	0.0	0.010675776662752216	0.0
27	0.0	0.0	0.0	0.037679211750890174	0.0
28	0.0	0.0	0.0	0.17395236091660962	0.0
29	0.0	0.0	0.0	0.3629764065335753	0.0
30	0.0	0.0	0.0	0.6750858772034489	0.0
31	0.0	0.0	0.0	1.3840830449826989	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	20	0.0018371774	37.0	4
GGTATCA	245	0.0	30.20408	1
GCAAGTC	25	0.0054823062	29.6	13
GATTTTG	25	0.0054823062	29.6	17
ATACGGC	40	5.908529E-5	27.750002	29
AGGACCG	35	8.8357343E-4	26.42857	5
ATGCCCC	35	8.8357343E-4	26.42857	37
GCGGTAA	235	0.0	23.617022	23
CGCGGTA	240	0.0	23.124998	22
AGCCGCG	250	0.0	22.940002	19
CCGCGGT	245	0.0	22.65306	21
GGTAATA	250	0.0	22.2	25
TACGGCT	50	2.6879588E-4	22.2	30
CGGTAAT	255	0.0	21.764706	24
TATACAC	85	5.1104507E-8	21.764706	37
CAGCCGC	260	0.0	21.346153	18
GCAGCCG	260	0.0	21.346153	17
TAATACG	245	0.0	21.142857	27
CAGTCGG	70	5.056516E-6	21.142857	10
CGTGCCA	265	0.0	20.943396	10
>>END_MODULE