FastQCFastQC Report
Fri 10 Feb 2017
ERR1632684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802405
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA70130.3890912419794663No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA69500.3855959121285172No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA59680.3311131515946749No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53100.29460637315142824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50290.27901609238767094No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA48860.27108224844027845No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA44370.24617108807399005No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA41780.23180139868675462No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT40380.22403399901797877No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA39050.21665496933264164No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA38590.21410282372718673No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG37820.20983075390935998No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC37520.20816631112319373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37470.20788890399216603No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA37260.20672379404184965No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT36450.20222979851920075No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA35820.1987344686682516No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC35570.19734743301311305No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT35100.19473980598145257No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC34070.18902521908228173No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC34060.1889697376560762No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT33350.18503055639548271No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA32370.1795933766273396No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA32230.178816636660462No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG31990.17748508243152897No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC31920.1770967124480902No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG31060.17232530979441357No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT30900.1714376069751249No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC29830.16550109437113192No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC29630.16439146584702108No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC29480.16355924445393794No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA29250.16228317165121045No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG29140.1616728759629495No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC28900.1603413217340165No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA28700.15923169320990566No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG27860.15457125340864014No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA27720.15379451344176254No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC27260.1512423678363076No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC26550.14730318657571412No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC26530.14719222372330304No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT26230.14552778093713678No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA26040.14447363383923148No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA25720.14269822820065411No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC24530.13609593848219462No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG23800.13204579436919006No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA23460.13015942587820162No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC22530.12499965324108621No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT22490.12477772753626405No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA22470.12466676468385297No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA22460.12461128325764743No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC22200.12316876617630332No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC21870.12133787911152044No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC21860.1212823976853149No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA21730.12056113914464285No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA21490.11922958491570985No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA21450.11900765921088767No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT21440.11895217778468213No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG21420.11884121493227105No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT21200.11762062355574912No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT20900.11595618076958286No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG20850.11567877363855515No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA20630.11445818226203323No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC20490.11368144229515564No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG20480.1136259608689501No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC20220.112183443787606No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT20100.1115176666731395No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC19770.10968677960835661No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC19620.10885455821527348No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT19310.10713463400290169No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT19170.1063578940360241No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG19120.10608048690499637No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC19090.10591404262637975No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG19050.1056921169215576No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT18970.10524826551191326No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG18680.10363930415195252No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA18620.10330641559471929No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG18600.10319545274230818No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC18570.10302900846369156No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT18370.10191937993958072No Hit
GGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTA18030.1000330114485923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG303.6002824E-430.83333429
AATTATC250.005497162729.59999825
GTATAAA250.005497162729.5999981
GGTATCA44200.028.2104071
CAATGCT1150.027.34782633
TACGGTA358.8713574E-426.4285722
GTGCTAG358.8713574E-426.4285721
TCAATGC1200.026.20833432
ATACGGC3500.025.37142829
TGTAACT751.3747922E-824.66666634
ATATGCT1103.6379788E-1223.54545432
ATGCCCC1200.023.12500237
TTGTAAC802.7279384E-823.12533
GCTTGAA502.7029283E-422.1999992
TACGGCT4450.021.20224830
CTTTTTA1400.021.1428592
CCCTATT450.003826639620.55555527
CTAGTTG450.003826639620.5555554
AAGATGT450.003826639620.55555536
GTAAGGA450.003826639620.5555552
Produced by FastQC (version 0.11.5)