FastQCFastQC Report
Fri 10 Feb 2017
ERR1632683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC22.0408163265306123No Hit
TCTCCCATCCACTTAAAATGACAAAACAGATCATTATAGCTAA11.0204081632653061No Hit
TTTTTGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATT11.0204081632653061No Hit
GTTCACATCTTCGCTCTGGCCAGCCCATTCTGTATTTAAAGCT11.0204081632653061No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA11.0204081632653061No Hit
ATTAAGGATCCACCTAACTTTTCAGCAATACAAGCCCGATTGA11.0204081632653061No Hit
CTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCCCGTAC11.0204081632653061No Hit
GCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTT11.0204081632653061No Hit
CTTTACTCCCTTCCTCCCCGCTCCCGTACTCTGCGTTGATACC11.0204081632653061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
GCCTCGGTGATAACGACATTGGGGCCACTAAAGATACGGAGAC11.0204081632653061No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAA11.0204081632653061No Hit
CTGTGAAAGACAGTGTCGCCGAAAGTCCAAGGGTTCCTGCGTA11.0204081632653061No Hit
CACCGAGGCAATCGCACTACATGGTCACTAAAGATGACCTGTC11.0204081632653061No Hit
TTTTTAGGCTGGGCACGGTGGCTCATACCTGTAATTCACCATG11.0204081632653061No Hit
GCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGATG11.0204081632653061No Hit
CTTAGAAGCAGAAAATGCAGCCAGCTGAGGAGTGGGTTTGCAG11.0204081632653061No Hit
CAGAGGAGAAATCCAGATCATTCTCAGCTTCCCAGGCAGACCC11.0204081632653061No Hit
ATTAGCTAGTAGGGGGGGTAACGGCTCACCTAGGCGACGATCC11.0204081632653061No Hit
GTATCAACGCAGAGTACGGGTGGTATCCTGTCTGAATATGGGC11.0204081632653061No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11.0204081632653061No Hit
ATCAACGCAGAGTACGGGACACACCGCGGGGACCAGGAGCTGT11.0204081632653061No Hit
CAATATGATGATGCTAAGACTTACAAAATTAACCTAATTTACA11.0204081632653061No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11.0204081632653061No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCAGTGATAACGACAT11.0204081632653061No Hit
CTCTTGGCACGAGCCGTTGCTAGCCAGCTGGACTGCAATTTCT11.0204081632653061No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11.0204081632653061No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC11.0204081632653061No Hit
GTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGA11.0204081632653061No Hit
AGATGAAGATGCAGAGGACCATTGTCATCCGCCGAGACTATCT11.0204081632653061No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11.0204081632653061No Hit
TTACTGGATACTGGCACTACTCCTGTTACAAGAAAAGCTGCTG11.0204081632653061No Hit
GGAATAGTCATTGGAGTAGAAGCCGTCATTGAGCTGTCTCTTA11.0204081632653061No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11.0204081632653061No Hit
CCTTATGTATGTGTTACAAGAATTTCCCCCACAACATCCTTTA11.0204081632653061No Hit
GTACAGTCTCAGTCCCATAAAATTGGATGTTGTGCCTACACAC11.0204081632653061No Hit
CATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACT11.0204081632653061No Hit
GATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAA11.0204081632653061No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG11.0204081632653061No Hit
ATGCTATAAAATCACCACGGTCTTTAGCCATGCACAAACGGTA11.0204081632653061No Hit
CCGTAGTCTGGCGGGAAGGTTGGCATCTGCAGCTGTCTCTTAT11.0204081632653061No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCTGTCTCTT11.0204081632653061No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCTAG11.0204081632653061No Hit
CCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTAC11.0204081632653061No Hit
CTTCATGGTCACTAAAGATGAGGAGTGACAAAACTGTCTCTTA11.0204081632653061No Hit
CCCGTCCACTCCGTCATCTTTAGTGGCCCCCTGTCTCTTATAC11.0204081632653061No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC11.0204081632653061No Hit
GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTC11.0204081632653061No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACC11.0204081632653061No Hit
CCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCAC11.0204081632653061No Hit
GATTTCCTCCATGGTCGGTTCTTGGGCGCTCTGATCCTGTCTC11.0204081632653061No Hit
TGGGTGGCCAGCGCGATGAACGCCGCAAGTGCTGTCTCTTATA11.0204081632653061No Hit
CTTAGCCTTCAGACCAATCATTTTTTTTGCCTTCTTTGAACGT11.0204081632653061No Hit
TTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGC11.0204081632653061No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC11.0204081632653061No Hit
TGATGATTTCATGACGAACCAGTGAACCTCCCATATTGCGGAA11.0204081632653061No Hit
GGTCTTCCTTGTCCTCGATGCCGATCGAGAGCCGCACCACCTC11.0204081632653061No Hit
GAAGTTACGCGTGCAATTTGCCGAGTTCCTTCAACGCAGTTCT11.0204081632653061No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTGTC11.0204081632653061No Hit
TATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAG11.0204081632653061No Hit
CCTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGC11.0204081632653061No Hit
ACGCAGAGTACGGGGATTTCACATCTGACTTAACAAACCGCCT11.0204081632653061No Hit
AAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAG11.0204081632653061No Hit
ACATTGCTTTGCATGCCAATAAATAAATTTTCTTTTAGTGTTG11.0204081632653061No Hit
GCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG11.0204081632653061No Hit
ACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGG11.0204081632653061No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGGATTTAGCCTTGGA11.0204081632653061No Hit
ATCTGCTCGGCCCGAGGGCACCAAGGTGAAGCGGCTGGTGACA11.0204081632653061No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA11.0204081632653061No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT11.0204081632653061No Hit
CTACACAACACTAAAAGAAAATTTATTTATTGGCATGCAAAGC11.0204081632653061No Hit
CCTTGGCAGCTTGGCCTTCCAAATAAGAACTTACATCACTGGC11.0204081632653061No Hit
TTGGGAGGCCGAGATGGGTGGATCACGAGGTCAGGAATTCAAG11.0204081632653061No Hit
CCGGTCAAGTACTTCTCCATCGACCGCGTATTCCGGAATGAGA11.0204081632653061No Hit
GACTTGAAGAACCCTGACTTTGTTTCTGCAAAGGCACCTGCAT11.0204081632653061No Hit
AGGCACTACGGTGCTGAAGCAACAAATGCCCTGCTTCCAGGAA11.0204081632653061No Hit
GTGAAAGGCCGAGGAAGGCGAGATTTCCCAGAAGAGGTCGCCA11.0204081632653061No Hit
CTACTGACCTTGTAATCCACCCACCTCACATCTTTTTAATTAT11.0204081632653061No Hit
CCAATAAGGAGGTGTTTCTTCTTGTCTTCAGGCCCCTTGATAT11.0204081632653061No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGAT11.0204081632653061No Hit
GTCTGCAGTCAAAGCCGATGCTGGGTGTCCTGTAGTGTAGCAG11.0204081632653061No Hit
TTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTATAAAG11.0204081632653061No Hit
GTATCAACGCAGAGTACGGGCACCTTCATGGCCAAGCCGATCG11.0204081632653061No Hit
TCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0204081632653061No Hit
CGCCGAGCCTGATCGAGATCGCCCTATGGGGCGGGGCGGTGTT11.0204081632653061No Hit
CCCGAGAGCATGCCCTTCTGGCTTACACACTGGGTGTGAAACA11.0204081632653061No Hit
ACACGGTGGATGCCCTGGCAGTCAGAGGTGATGAAGGACGTGC11.0204081632653061No Hit
AGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC11.0204081632653061No Hit
TGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTCTGTC11.0204081632653061No Hit
CACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGG11.0204081632653061No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG11.0204081632653061No Hit
TCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAGCT11.0204081632653061No Hit
GCAGAATATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCTA11.0204081632653061No Hit
TGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG11.0204081632653061No Hit
AGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTG11.0204081632653061No Hit
GTACTTAGATGTTTCAGTTCCCCCGGATCGCCTCATTAACCTA11.0204081632653061No Hit
CCTCTAAGCATCAGGTAACATCAAATCGTACCCCAAACCGACA11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACT50.037.037
CCGGATT50.037.019
ACTCGAC50.037.036
TGCGTCG50.037.08
TGCAACT50.037.032
GTCGTAG50.037.011
CAACTCG50.037.034
GTCCGGA50.037.017
ATTGGAG50.037.023
GGATTGG50.037.021
CGTAGTC50.037.013
CGGATTG50.037.020
GTCTGCA50.037.029
TGGAGTC50.037.025
GCAACTC50.037.033
TAGTCCG50.037.015
GAGTCTG50.037.027
GATTGGA50.037.022
AACTCGA50.037.035
TCTGCAA50.037.030