FastQCFastQC Report
Fri 10 Feb 2017
ERR1632682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16539
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA860.5199830703186409No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT570.3446399419553782TruSeq Adapter, Index 11 (95% over 23bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA520.3144083680996433No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA510.30836205332849626No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA490.2962694237862023No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT490.2962694237862023No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG490.2962694237862023No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA480.2902231090150553No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT470.2841767942439083No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC450.27208416470161434No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA450.27208416470161434No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT440.26603784993046736No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC440.26603784993046736No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA420.2539452203881734No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC410.24789890561702643No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT380.22975996130358547No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC370.22371364653243847No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA370.22371364653243847No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC370.22371364653243847TruSeq Adapter, Index 11 (95% over 21bp)
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA360.21766733176129147No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT360.21766733176129147No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA360.21766733176129147No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA350.21162101699014452No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA340.2055747022189975No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC330.19952838744785054No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC310.18743575790555655No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA300.18138944313440958No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA290.17534312836326257No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG290.17534312836326257No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA290.17534312836326257No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC280.1692968135921156No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA280.1692968135921156No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC270.16325049882096862No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA270.16325049882096862No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT260.15720418404982164No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC260.15720418404982164No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA260.15720418404982164No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT250.15115786927867467No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG250.15115786927867467No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA250.15115786927867467No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG250.15115786927867467No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC240.14511155450752766No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC240.14511155450752766No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC230.13906523973638069No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC230.13906523973638069No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC230.13906523973638069No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG230.13906523973638069No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC220.13301892496523368No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT210.1269726101940867No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA210.1269726101940867No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA200.12092629542293971No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC200.12092629542293971No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT200.12092629542293971No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT200.12092629542293971No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG190.11487998065179274No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC190.11487998065179274No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG190.11487998065179274No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC190.11487998065179274No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT190.11487998065179274No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT180.10883366588064573No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA180.10883366588064573No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT180.10883366588064573No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG180.10883366588064573No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC180.10883366588064573No Hit
CAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAAT180.10883366588064573No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT180.10883366588064573No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGC170.10278735110949876No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC170.10278735110949876No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC170.10278735110949876No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG170.10278735110949876No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC170.10278735110949876No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC170.10278735110949876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCAG358.5028994E-426.4285714
CGCGGTA358.5028994E-426.4285722
AGCCGCG358.5028994E-426.4285719
CGGTAAT358.5028994E-426.4285724
GGTAATA358.5028994E-426.4285725
CAGCCGC358.5028994E-426.4285718
CAGCAGC400.00185212923.12515
GCCAGCA400.00185212923.12513
GCAGCCG400.00185212923.12517
TGCCAGC400.00185212923.12512
CGTGCCA400.00185212923.12510
TAATACG400.00185212923.12527
GTAATAC400.00185212923.12526
CCGCGGT400.00185212923.12521
GTGCCAG400.00185212923.12511
GCGGTAA450.003670648720.55555723
AGCAGCC450.003670648720.55555716
GCTAACT450.003670648720.5555572
GGCTAAC450.003670648720.5555571
GCCGCGG450.003670648720.55555720
Produced by FastQC (version 0.11.5)