##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632682.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16539
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37505290525425	33.0	31.0	34.0	31.0	34.0
2	32.56907914626036	34.0	31.0	34.0	31.0	34.0
3	32.641151218332425	34.0	31.0	34.0	31.0	34.0
4	36.18592417921277	37.0	35.0	37.0	35.0	37.0
5	35.98712134953746	37.0	35.0	37.0	35.0	37.0
6	35.944313440957735	37.0	35.0	37.0	35.0	37.0
7	35.98905617026423	37.0	35.0	37.0	35.0	37.0
8	35.97176371001874	37.0	35.0	37.0	35.0	37.0
9	37.617993832758934	39.0	37.0	39.0	35.0	39.0
10	37.5603724529899	39.0	37.0	39.0	35.0	39.0
11	37.630328314892076	39.0	37.0	39.0	35.0	39.0
12	37.51587157627426	39.0	37.0	39.0	35.0	39.0
13	37.624161073825505	39.0	37.0	39.0	35.0	39.0
14	38.82840558679485	40.0	38.0	41.0	36.0	41.0
15	38.8597859604571	40.0	38.0	41.0	36.0	41.0
16	38.781425721023034	40.0	38.0	41.0	35.0	41.0
17	38.77187254368462	40.0	38.0	41.0	35.0	41.0
18	38.834330975270575	40.0	38.0	41.0	36.0	41.0
19	38.85077695144809	40.0	38.0	41.0	36.0	41.0
20	38.806397001027875	40.0	38.0	41.0	35.0	41.0
21	38.72767398270754	40.0	38.0	41.0	35.0	41.0
22	38.70360964991838	40.0	38.0	41.0	35.0	41.0
23	38.649676522159744	40.0	38.0	41.0	35.0	41.0
24	38.5941108894129	40.0	38.0	41.0	35.0	41.0
25	38.590422637402504	40.0	38.0	41.0	35.0	41.0
26	38.44899933490537	40.0	38.0	41.0	35.0	41.0
27	38.228429772053936	40.0	38.0	41.0	34.0	41.0
28	38.254549851865285	40.0	38.0	41.0	34.0	41.0
29	38.17153395005744	40.0	38.0	41.0	34.0	41.0
30	38.13144688312474	40.0	38.0	41.0	34.0	41.0
31	38.06366769454018	40.0	38.0	41.0	34.0	41.0
32	37.974242699074914	40.0	37.0	41.0	33.0	41.0
33	37.875566842009796	40.0	37.0	41.0	33.0	41.0
34	37.885361871939054	40.0	37.0	41.0	33.0	41.0
35	37.777737469012635	40.0	37.0	41.0	33.0	41.0
36	37.6979261140335	40.0	37.0	41.0	33.0	41.0
37	37.63419795634561	39.0	37.0	41.0	33.0	41.0
38	37.5698651671806	39.0	37.0	41.0	33.0	41.0
39	37.43521373722716	39.0	36.0	41.0	32.0	41.0
40	37.36175101275772	39.0	36.0	40.0	32.0	41.0
41	37.26694479714614	39.0	36.0	40.0	32.0	41.0
42	37.231815708325776	39.0	36.0	40.0	32.0	41.0
43	36.36549972791583	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	3.0
21	2.0
22	1.0
23	10.0
24	16.0
25	23.0
26	33.0
27	66.0
28	71.0
29	111.0
30	142.0
31	200.0
32	276.0
33	375.0
34	588.0
35	896.0
36	1650.0
37	2974.0
38	5187.0
39	3912.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.76794243908338	16.300864623012274	13.755366104359393	28.175826833544953
2	23.502025515448334	19.928653485700465	29.83856339561037	26.730757603240825
3	21.917891045407824	18.592417921276983	29.56647923090876	29.923211802406435
4	15.84739101517625	17.002237136465325	34.826773081806635	32.32359876655179
5	17.03246871032106	32.03942197230788	33.115666001572045	17.81244331579902
6	30.884575851018802	35.11094987605055	16.1497067537336	17.854767519197047
7	28.744180422032773	26.821452324808032	19.80772719027753	24.626640062881673
8	26.36193240220086	29.076727734445857	21.591390047765888	22.9699498155874
9	27.813047947276136	12.07449059798053	16.911542414898122	43.20091903984522
10	20.533284962815166	21.95416893403471	26.869822842977204	30.64272326017293
11	40.74611524275954	18.67706632807304	17.407340226132174	23.169478203035247
12	22.141604691940262	23.762017050607653	26.386117661285446	27.710260596166634
13	38.406191426325655	16.730152971763708	18.531954773565513	26.331700828345124
14	24.336416953866618	20.7569986093476	22.377410968014992	32.529173468770786
15	31.507346272446945	23.06669085192575	18.70125158715763	26.724711288469678
16	24.106656992563032	24.360602212951207	22.879255094020195	28.653485700465563
17	28.314892073281335	23.22994135074672	19.983070318640788	28.47209625733116
18	27.129814378136523	19.922607170929318	22.10532680331338	30.842251647620778
19	28.816736199286535	22.667634077030048	21.917891045407824	26.59773867827559
20	32.20267247112885	19.287744119958887	21.585343732994737	26.924239675917526
21	28.1818731483161	20.642118628695812	21.74254791704456	29.433460305943527
22	29.76600761835661	21.615575306850474	20.26120079811355	28.357216276679363
23	30.080415986456256	21.168148013785597	20.587701795755486	28.16373420400266
24	28.478142572102307	20.388173408307637	22.637402503174314	28.496281516415745
25	29.270209807122562	21.228611161497067	22.093234173771087	27.40794485760929
26	29.294395066207144	21.98440050789044	21.633714251163916	27.087490174738498
27	26.694479714613944	21.815103694298323	24.8745389684987	26.61587762258903
28	26.507043956708387	23.33272870185622	20.696535461636135	29.463691879799264
29	27.65584376322631	23.755970735836506	20.44259024124796	28.14559525968922
30	27.389805913295845	21.198379587641334	24.058286474393856	27.353528024668964
31	28.786504625430798	21.180240643327892	22.238345728278613	27.794909002962694
32	26.265191365862506	21.470463752342948	22.06300259991535	30.201342281879196
33	26.0717092931858	20.932341737710868	24.53594534131447	28.46000362778886
34	26.19263558860874	21.035129088820366	23.677368643811597	29.094866678759296
35	25.70288409214584	23.967591752826653	24.475482193603	25.854041961424514
36	26.44053449422577	21.180240643327892	25.140576818429167	27.23864804401717
37	27.25678698833061	21.252796420581653	24.106656992563032	27.383759598524698
38	26.24100610677792	20.327710260596167	25.430799927444227	28.00048370518169
39	25.7754398693996	19.184956768849386	26.19263558860874	28.846967773142268
40	25.31591994679243	19.686800894854585	28.71999516294818	26.277283995404797
41	23.278311868915896	19.269605175645445	28.937662494709475	28.514420460729184
42	20.696535461636135	20.067718725436844	31.634318882641033	27.60142693028599
43	19.366346211983796	22.141604691940262	29.560432916137614	28.93161617993833
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	1.0
9	1.0
10	3.5
11	6.0
12	6.0
13	8.5
14	11.0
15	12.5
16	14.0
17	17.0
18	20.0
19	20.0
20	19.0
21	18.0
22	15.5
23	13.0
24	12.5
25	12.0
26	12.0
27	10.5
28	9.0
29	12.0
30	15.0
31	16.5
32	18.0
33	18.0
34	38.0
35	58.0
36	58.5
37	59.0
38	106.5
39	154.0
40	154.0
41	209.0
42	264.0
43	372.0
44	480.0
45	906.5
46	1333.0
47	1333.0
48	1495.5
49	1658.0
50	1924.0
51	2190.0
52	2255.5
53	2321.0
54	2321.0
55	2061.5
56	1802.0
57	1715.5
58	1629.0
59	1550.0
60	1471.0
61	1471.0
62	1306.0
63	1141.0
64	886.5
65	632.0
66	549.0
67	466.0
68	466.0
69	376.0
70	286.0
71	239.0
72	192.0
73	146.0
74	100.0
75	100.0
76	85.0
77	70.0
78	52.0
79	34.0
80	30.5
81	27.0
82	27.0
83	22.5
84	18.0
85	12.0
86	6.0
87	6.0
88	6.0
89	6.0
90	4.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	16539.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.61061732873814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.54386854650573	49.204909607594175
2	7.860837813165636	9.371787895277828
3	3.2964803732630084	5.895156901868312
4	1.5924535956993608	3.7970856762803074
5	1.0650167359772797	3.174315254852168
6	0.7302971903844203	2.612007981135498
7	0.4564357439902627	1.9045891529113006
8	0.34486256212597627	1.64459761775198
9	0.3752916117253271	2.013422818791946
>10	1.6938837610305304	18.89473365983433
>50	0.0405720661324678	1.4873934337021586
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	86	0.5199830703186409	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	57	0.3446399419553782	TruSeq Adapter, Index 11 (95% over 23bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	52	0.3144083680996433	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	51	0.30836205332849626	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	49	0.2962694237862023	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	49	0.2962694237862023	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	49	0.2962694237862023	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	48	0.2902231090150553	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	47	0.2841767942439083	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	45	0.27208416470161434	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	45	0.27208416470161434	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	44	0.26603784993046736	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	44	0.26603784993046736	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	42	0.2539452203881734	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	41	0.24789890561702643	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	38	0.22975996130358547	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	37	0.22371364653243847	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	37	0.22371364653243847	No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	37	0.22371364653243847	TruSeq Adapter, Index 11 (95% over 21bp)
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	36	0.21766733176129147	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	36	0.21766733176129147	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	36	0.21766733176129147	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	35	0.21162101699014452	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	34	0.2055747022189975	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	33	0.19952838744785054	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	31	0.18743575790555655	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	30	0.18138944313440958	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	29	0.17534312836326257	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	29	0.17534312836326257	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	29	0.17534312836326257	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	28	0.1692968135921156	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	28	0.1692968135921156	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	27	0.16325049882096862	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	27	0.16325049882096862	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT	26	0.15720418404982164	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	26	0.15720418404982164	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	26	0.15720418404982164	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	25	0.15115786927867467	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	25	0.15115786927867467	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	25	0.15115786927867467	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	25	0.15115786927867467	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	24	0.14511155450752766	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	24	0.14511155450752766	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	23	0.13906523973638069	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	23	0.13906523973638069	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	23	0.13906523973638069	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	23	0.13906523973638069	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	22	0.13301892496523368	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT	21	0.1269726101940867	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	21	0.1269726101940867	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	20	0.12092629542293971	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	20	0.12092629542293971	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	20	0.12092629542293971	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	20	0.12092629542293971	No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG	19	0.11487998065179274	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	19	0.11487998065179274	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	19	0.11487998065179274	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	19	0.11487998065179274	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	19	0.11487998065179274	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	18	0.10883366588064573	No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA	18	0.10883366588064573	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	18	0.10883366588064573	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	18	0.10883366588064573	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	18	0.10883366588064573	No Hit
CAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAAT	18	0.10883366588064573	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	18	0.10883366588064573	No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGC	17	0.10278735110949876	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	17	0.10278735110949876	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	17	0.10278735110949876	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	17	0.10278735110949876	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	17	0.10278735110949876	No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC	17	0.10278735110949876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.006046314771146986	0.0
17	0.0	0.0	0.0	0.006046314771146986	0.0
18	0.0	0.0	0.0	0.006046314771146986	0.0
19	0.0	0.0	0.0	0.006046314771146986	0.0
20	0.0	0.0	0.0	0.006046314771146986	0.0
21	0.0	0.0	0.0	0.006046314771146986	0.0
22	0.0	0.0	0.0	0.006046314771146986	0.0
23	0.0	0.0	0.0	0.006046314771146986	0.0
24	0.0	0.0	0.0	0.006046314771146986	0.0
25	0.0	0.0	0.0	0.006046314771146986	0.0
26	0.0	0.0	0.0	0.006046314771146986	0.0
27	0.0	0.0	0.0	0.04837051816917589	0.0
28	0.0	0.0	0.0	0.18138944313440958	0.0
29	0.0	0.0	0.0	0.44138097829372996	0.0
30	0.0	0.0	0.0	0.870669327045166	0.0
31	0.0	0.0	0.0	1.4692544893887176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGCAG	35	8.5028994E-4	26.42857	14
CGCGGTA	35	8.5028994E-4	26.42857	22
AGCCGCG	35	8.5028994E-4	26.42857	19
CGGTAAT	35	8.5028994E-4	26.42857	24
GGTAATA	35	8.5028994E-4	26.42857	25
CAGCCGC	35	8.5028994E-4	26.42857	18
CAGCAGC	40	0.001852129	23.125	15
GCCAGCA	40	0.001852129	23.125	13
GCAGCCG	40	0.001852129	23.125	17
TGCCAGC	40	0.001852129	23.125	12
CGTGCCA	40	0.001852129	23.125	10
TAATACG	40	0.001852129	23.125	27
GTAATAC	40	0.001852129	23.125	26
CCGCGGT	40	0.001852129	23.125	21
GTGCCAG	40	0.001852129	23.125	11
GCGGTAA	45	0.0036706487	20.555557	23
AGCAGCC	45	0.0036706487	20.555557	16
GCTAACT	45	0.0036706487	20.555557	2
GGCTAAC	45	0.0036706487	20.555557	1
GCCGCGG	45	0.0036706487	20.555557	20
>>END_MODULE