FastQCFastQC Report
Fri 10 Feb 2017
ERR1632680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20714
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA670.32345273727913487No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA550.26552090373660325No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA500.2413826397605484No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT470.2268996813749155No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA470.2268996813749155No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC390.18827845901322776No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA380.1834508062180168No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC350.1689678478323839No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT330.15931254224196195No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC320.15448488944675098No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT310.14965723665154002No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA290.14000193106111808No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT270.13034662547069614No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT270.13034662547069614No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG270.13034662547069614No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA260.12551897267548517No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA260.12551897267548517No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC250.1206913198802742No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC240.11586366708506324No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC240.11586366708506324No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC240.11586366708506324No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA240.11586366708506324No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT230.11103601428985228No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA220.10620836149464132No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC210.10138070869943033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAA303.47988E-430.83333223
GCAGCCG303.47988E-430.83333217
CGCGGTA303.47988E-430.83333222
CGTGCCA303.47988E-430.83333210
AGCCGCG303.47988E-430.83333219
CGGTAAT303.47988E-430.83333224
GGTAATA303.47988E-430.83333225
GGTATCA405.667464E-527.7500021
CCGTGCC358.577432E-426.4285729
GTGCCAG358.577432E-426.42857211
CAGCCGC358.577432E-426.42857218
AACTCCG400.001868209323.1250025
TCCGTGC400.001868209323.1250028
GCCGCGG400.001868209323.12500220
GTAATAC400.001868209323.12500226
CTCCGTG400.001868209323.1250027
CCGCGGT400.001868209323.12500221
GTATCAA502.5800205E-422.22
CCAGCAG450.003702215920.55555714
AGCAGCC450.003702215920.55555716
Produced by FastQC (version 0.11.5)