##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632680.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20714
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01284155643526	34.0	33.0	34.0	31.0	34.0
2	33.162305686974996	34.0	33.0	34.0	31.0	34.0
3	33.235106691126774	34.0	34.0	34.0	31.0	34.0
4	36.510572559621515	37.0	37.0	37.0	35.0	37.0
5	36.48329632132857	37.0	37.0	37.0	35.0	37.0
6	36.51665540214348	37.0	37.0	37.0	35.0	37.0
7	36.501931061118086	37.0	37.0	37.0	35.0	37.0
8	36.50420005793183	37.0	37.0	37.0	35.0	37.0
9	38.29400405522835	39.0	39.0	39.0	37.0	39.0
10	38.31568021627884	39.0	39.0	39.0	37.0	39.0
11	38.37926040359177	39.0	39.0	39.0	37.0	39.0
12	38.33388046731679	39.0	39.0	39.0	37.0	39.0
13	38.382736313604326	39.0	39.0	39.0	37.0	39.0
14	39.904943516462296	41.0	40.0	41.0	38.0	41.0
15	39.8856811818094	41.0	40.0	41.0	38.0	41.0
16	39.86318431978372	41.0	40.0	41.0	38.0	41.0
17	39.85999806893888	41.0	40.0	41.0	38.0	41.0
18	39.88601911750507	41.0	40.0	41.0	38.0	41.0
19	39.871246499951724	41.0	40.0	41.0	38.0	41.0
20	39.85077725210003	41.0	40.0	41.0	38.0	41.0
21	39.78758327701072	41.0	40.0	41.0	38.0	41.0
22	39.78029352128995	41.0	40.0	41.0	38.0	41.0
23	39.76721058221493	41.0	40.0	41.0	38.0	41.0
24	39.72062373274114	41.0	40.0	41.0	38.0	41.0
25	39.69653374529304	41.0	40.0	41.0	37.0	41.0
26	39.58018731292845	41.0	40.0	41.0	37.0	41.0
27	39.46968234044608	41.0	39.0	41.0	37.0	41.0
28	39.43323356184223	41.0	39.0	41.0	37.0	41.0
29	39.373756879405235	41.0	39.0	41.0	36.0	41.0
30	39.34049435164623	41.0	39.0	41.0	36.0	41.0
31	39.290045379936274	41.0	39.0	41.0	36.0	41.0
32	39.22936178430047	41.0	39.0	41.0	35.0	41.0
33	39.19141643333011	41.0	39.0	41.0	35.0	41.0
34	39.137443275079654	41.0	39.0	41.0	35.0	41.0
35	39.01897267548518	41.0	39.0	41.0	35.0	41.0
36	38.97615139519166	41.0	39.0	41.0	35.0	41.0
37	38.91488848122043	41.0	39.0	41.0	35.0	41.0
38	38.84421164429854	40.0	39.0	41.0	35.0	41.0
39	38.71840301245535	40.0	38.0	41.0	35.0	41.0
40	38.62629139712272	40.0	38.0	41.0	35.0	41.0
41	38.607077338997776	40.0	38.0	41.0	35.0	41.0
42	38.512648450323454	40.0	38.0	41.0	35.0	41.0
43	37.821569952689	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	1.0
20	0.0
21	4.0
22	6.0
23	7.0
24	11.0
25	13.0
26	32.0
27	39.0
28	56.0
29	89.0
30	113.0
31	135.0
32	175.0
33	237.0
34	357.0
35	516.0
36	842.0
37	1624.0
38	3950.0
39	12506.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.19300955875253	16.138843294390266	12.392584725306556	28.275562421550642
2	22.149271024427925	19.252679347301342	30.650767596794438	27.947282031476295
3	21.22236168774742	18.765086414985035	28.898329632132857	31.11422226513469
4	16.60712561552573	15.091242637829488	33.13700878632809	35.16462296031669
5	17.58713913295356	32.04595925461041	32.673554117987834	17.6933474944482
6	35.323935502558655	32.72665829873516	15.21193395770976	16.73747224099643
7	30.061793955778697	26.943130250072418	19.571304431785265	23.423771362363617
8	27.51279327990731	30.539731582504587	20.068552669691996	21.878922467896107
9	28.290045379936274	12.576035531524573	16.75678285217727	42.37713623636188
10	19.44578545910978	22.994110263589842	28.589359853239355	28.970744424061024
11	39.89572269962344	18.707154581442502	17.89610891184706	23.501013807086995
12	22.825142415757462	23.356184223230667	25.605870425798976	28.2128029352129
13	36.680505938012935	16.964371922371342	19.783721154774547	26.57140098484117
14	24.408612532586655	19.39750893115767	22.202375205175244	33.99150333108043
15	30.655595249589652	23.061697402722796	19.011296707540794	27.271410640146758
16	26.730713527083132	23.43342666795404	21.23684464613305	28.599015158829776
17	27.401757265617455	23.43342666795404	20.700975185864632	28.46384088056387
18	27.030028000386213	20.396833059766344	22.443757844935792	30.129381094911658
19	28.285217727141067	21.772714106401466	22.63203630394902	27.31003186250845
20	30.95973737568794	19.9382060442213	21.92719899584822	27.174857584242538
21	28.30935599111712	21.217534034952205	21.309259438061215	29.16385053586946
22	29.443854397991696	22.23616877474172	20.02027614173989	28.299700685526695
23	29.159022883074247	21.04856618711982	21.217534034952205	28.574876894853723
24	28.207975282417692	20.78787293617843	22.096166843680603	28.90798493772328
25	29.289369508544944	21.04856618711982	22.12996041324708	27.532103891088155
26	29.120401660712563	21.676161050497246	21.647195133725983	27.556242155064208
27	27.39210196002703	21.299604132470794	23.206526986579128	28.101766920923048
28	26.711402915902287	22.77203823501014	20.898908950468282	29.61764989861929
29	27.590035724630685	23.269286472916868	21.623056869749927	27.51762093270252
30	27.13140870908564	21.92237134305301	22.979627305204207	27.96659264265714
31	28.49763445013035	21.806507675967946	21.82581828714879	27.87003958675292
32	26.43139905378005	21.58443564738824	22.02857970454765	29.955585594284056
33	26.11277396929613	21.632712175340348	23.732741141257122	28.5217727141064
34	27.276238292941972	21.18374046538573	22.86859129091436	28.671429950757943
35	26.156222844453026	23.22583759775997	24.003089697788933	26.614849859998067
36	26.759679443854402	20.98580670078208	24.664478130732835	27.590035724630685
37	27.797624794824756	21.063049145505456	23.592739210196005	27.546586849473787
38	26.13208458047697	20.918219561649128	25.562421550642078	27.387274307231824
39	25.910012551897267	19.47957902867626	26.441054359370476	28.169354060056
40	24.87689485372212	20.729941102635898	27.290721251327604	27.102442792314374
41	23.462392584725304	20.647871005117313	27.478999710340833	28.410736699816546
42	22.004441440571597	19.856135946702715	29.467992661967752	28.671429950757943
43	21.000289659167713	20.459592546104084	29.043159215989185	29.49695857873902
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	2.0
9	3.0
10	3.5
11	4.0
12	4.0
13	7.0
14	10.0
15	17.5
16	25.0
17	29.5
18	34.0
19	34.0
20	38.0
21	42.0
22	38.5
23	35.0
24	35.5
25	36.0
26	36.0
27	37.0
28	38.0
29	65.0
30	92.0
31	99.5
32	107.0
33	107.0
34	148.5
35	190.0
36	213.5
37	237.0
38	290.0
39	343.0
40	343.0
41	437.0
42	531.0
43	607.0
44	683.0
45	1000.5
46	1318.0
47	1318.0
48	1523.0
49	1728.0
50	1883.0
51	2038.0
52	2220.5
53	2403.0
54	2403.0
55	2233.0
56	2063.0
57	2038.5
58	2014.0
59	1905.5
60	1797.0
61	1797.0
62	1675.0
63	1553.0
64	1299.0
65	1045.0
66	921.0
67	797.0
68	797.0
69	703.0
70	609.0
71	503.0
72	397.0
73	314.0
74	231.0
75	231.0
76	180.5
77	130.0
78	105.0
79	80.0
80	63.0
81	46.0
82	46.0
83	36.0
84	26.0
85	19.5
86	13.0
87	9.0
88	5.0
89	5.0
90	5.5
91	6.0
92	4.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	20714.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.09829101091049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.39288206559665	68.02645553731776
2	5.779356721436275	8.795983392874385
3	1.846095286430248	4.214540890219175
4	0.9135316881304321	2.780728010041518
5	0.494829664403984	1.8827845901322777
6	0.38063820338768	1.7379550062759486
7	0.21569498191968536	1.1489813652602103
8	0.1268794011292267	0.7724244472337549
9	0.1268794011292267	0.8689775031379743
>10	0.7105246463236694	9.182195616491262
>50	0.012687940112922666	0.5889736410157381
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	67	0.32345273727913487	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	55	0.26552090373660325	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	50	0.2413826397605484	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	47	0.2268996813749155	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	47	0.2268996813749155	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	39	0.18827845901322776	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	38	0.1834508062180168	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	35	0.1689678478323839	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	33	0.15931254224196195	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	32	0.15448488944675098	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	31	0.14965723665154002	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	29	0.14000193106111808	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	27	0.13034662547069614	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	27	0.13034662547069614	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	27	0.13034662547069614	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	26	0.12551897267548517	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	26	0.12551897267548517	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	25	0.1206913198802742	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	24	0.11586366708506324	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	24	0.11586366708506324	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	24	0.11586366708506324	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	24	0.11586366708506324	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	23	0.11103601428985228	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	22	0.10620836149464132	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	21	0.10138070869943033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.004827652795210968	0.0
17	0.0	0.0	0.0	0.004827652795210968	0.0
18	0.0	0.0	0.0	0.009655305590421936	0.0
19	0.0	0.0	0.0	0.009655305590421936	0.0
20	0.0	0.0	0.0	0.019310611180843873	0.0
21	0.0	0.0	0.0	0.019310611180843873	0.0
22	0.0	0.0	0.0	0.02413826397605484	0.0
23	0.0	0.0	0.0	0.02896591677126581	0.0
24	0.0	0.0	0.0	0.038621222361687746	0.0
25	0.0	0.0	0.0	0.038621222361687746	0.0
26	0.0	0.0	0.0	0.038621222361687746	0.0
27	0.0	0.0	0.0	0.08207009751858646	0.0
28	0.0	0.0	0.0	0.17862315342280582	0.0
29	0.0	0.0	0.0	0.3620739596408226	0.0
30	0.0	0.0	0.0	0.5889736410157381	0.0
31	0.0	0.0	0.0	1.071738920536835	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGTAA	30	3.47988E-4	30.833332	23
GCAGCCG	30	3.47988E-4	30.833332	17
CGCGGTA	30	3.47988E-4	30.833332	22
CGTGCCA	30	3.47988E-4	30.833332	10
AGCCGCG	30	3.47988E-4	30.833332	19
CGGTAAT	30	3.47988E-4	30.833332	24
GGTAATA	30	3.47988E-4	30.833332	25
GGTATCA	40	5.667464E-5	27.750002	1
CCGTGCC	35	8.577432E-4	26.428572	9
GTGCCAG	35	8.577432E-4	26.428572	11
CAGCCGC	35	8.577432E-4	26.428572	18
AACTCCG	40	0.0018682093	23.125002	5
TCCGTGC	40	0.0018682093	23.125002	8
GCCGCGG	40	0.0018682093	23.125002	20
GTAATAC	40	0.0018682093	23.125002	26
CTCCGTG	40	0.0018682093	23.125002	7
CCGCGGT	40	0.0018682093	23.125002	21
GTATCAA	50	2.5800205E-4	22.2	2
CCAGCAG	45	0.0037022159	20.555557	14
AGCAGCC	45	0.0037022159	20.555557	16
>>END_MODULE