FastQCFastQC Report
Fri 10 Feb 2017
ERR1632678.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632678.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158996
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7390.46479156708344865No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6100.3836574505018994No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5640.3547259050542152No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5240.32956803944753327No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4710.29623386751867975No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4420.2779944149538353No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4380.2754786283931671No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4350.27359178847266596No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4290.2698181086316637No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4250.2673023220709955No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4240.2666733754308284No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4140.2603839090291579No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4110.25849706910865683No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4080.2566102291881557No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3990.25094970942665223No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3920.24654708294548291No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3510.2207602706986339No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3500.2201313240584669No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3360.21132607109612822No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3320.20881028453546002No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3260.20503660469445772No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3250.20440765805429068No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3180.20000503157312136No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3030.1905708319706156No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3020.18994188533044856No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3020.18994188533044856No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3010.1893129386902815No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2970.18679715212961331No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2900.18239452564844397No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2880.1811366323681099No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2830.17799189916727465No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2810.17673400588694055No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2780.1748471659664394No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2770.17421821932627235No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT2750.17296032604593828RNA PCR Primer, Index 29 (95% over 23bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2670.16792875292460188No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2600.16352612644343253No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2590.16289717980326548No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2580.16226823316309844No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2410.15157614028025862No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2330.14654456715892222No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2280.143399833958087No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2270.14277088731791995No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2260.1421419406777529No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2260.1421419406777529No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2260.1421419406777529No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2260.1421419406777529No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2250.14151299403758585No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2250.14151299403758585No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2230.14025510075725175No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2220.1396261541170847No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2200.1383682608367506No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2180.1371103675564165No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2140.1345945809957483No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2060.12956300787441194No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2060.12956300787441194No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2000.12578932803340964No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1970.12390248811290851No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1960.12327354147274146No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1950.1226445948325744No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1950.1226445948325744No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1950.1226445948325744No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1940.12201564819240736No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1940.12201564819240736No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA1930.1213867015522403No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1900.11949986163173917No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1890.11887091499157211No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1880.11824196835140506No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1860.11698407507107096No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1860.11698407507107096No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1850.11635512843090393No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1810.11383934187023573No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT1810.11383934187023573No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1800.11321039523006868No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1800.11321039523006868No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1770.11132355530956753No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT1760.1106946086694005No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG1710.10754987546856526No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1710.10754987546856526No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1700.1069209288283982No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG1700.1069209288283982No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT1690.10629198218823115No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT1670.10503408890789705No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1660.10440514226773001No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC1640.10314724898739591No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1630.10251830234722886No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA1620.1018893557070618No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC1620.1018893557070618No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1610.10126040906689476No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT1610.10126040906689476No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG1600.10063146242672771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCATG200.001837168537.07
TAGGACC200.001837168537.04
CTCCCAT200.001837168537.06
CTTAGGA200.001837168537.02
CACTCCC200.001837168537.04
TTAGGAC200.001837168537.03
GAGGGCT200.001837168537.034
GCAGTCG251.2288458E-436.9999969
TGGGCAG251.2288458E-436.9999966
GGTATCA3500.032.242861
ATACGGC352.375477E-531.71428529
TTTTTTG303.5856626E-430.8333321
GGTGATT250.005482282529.59999815
TAACGGC250.005482282529.59999830
TGATTCC250.005482282529.59999817
ATGGTGT250.005482282529.59999811
ACTCCCA250.005482282529.5999985
CCCATGG250.005482282529.5999988
TCTACGC250.005482282529.59999810
CGCTACA250.005482282529.59999824
Produced by FastQC (version 0.11.5)