FastQCFastQC Report
Fri 10 Feb 2017
ERR1632676.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632676.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT130.4679625629949604TruSeq Adapter, Index 6 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA90.32397408207343414No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA80.28797696184305255No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA70.25197984161267095No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA70.25197984161267095No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA60.21598272138228944No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT60.21598272138228944No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC60.21598272138228944No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC50.17998560115190784RNA PCR Primer, Index 6 (95% over 22bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG50.17998560115190784No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA50.17998560115190784No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG50.17998560115190784No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT50.17998560115190784No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA50.17998560115190784No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT40.14398848092152627No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC40.14398848092152627No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC40.14398848092152627No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA40.14398848092152627No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC40.14398848092152627No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.14398848092152627No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG40.14398848092152627No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC40.14398848092152627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40.14398848092152627No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC40.14398848092152627No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC40.14398848092152627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10799136069114472No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT30.10799136069114472No Hit
CTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA30.10799136069114472No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30.10799136069114472No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT30.10799136069114472No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG30.10799136069114472No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG30.10799136069114472No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC30.10799136069114472No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC30.10799136069114472No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC30.10799136069114472No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC30.10799136069114472No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC30.10799136069114472No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT30.10799136069114472No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA30.10799136069114472No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA30.10799136069114472No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG30.10799136069114472No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG30.10799136069114472No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA30.10799136069114472No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA30.10799136069114472No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC30.10799136069114472No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG30.10799136069114472No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG30.10799136069114472No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC30.10799136069114472No Hit
ATGCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATG30.10799136069114472No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC30.10799136069114472No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG30.10799136069114472No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC30.10799136069114472No Hit
CGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGAT30.10799136069114472No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA30.10799136069114472No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTCTG30.10799136069114472No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers