##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632676.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2778
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.926205903527716	34.0	33.0	34.0	31.0	34.0
2	33.08927285817135	34.0	33.0	34.0	31.0	34.0
3	33.16486681065515	34.0	33.0	34.0	31.0	34.0
4	36.42872570194385	37.0	37.0	37.0	35.0	37.0
5	36.37257019438445	37.0	37.0	37.0	35.0	37.0
6	36.43304535637149	37.0	37.0	37.0	35.0	37.0
7	36.48272138228942	37.0	37.0	37.0	35.0	37.0
8	36.402447804175665	37.0	37.0	37.0	35.0	37.0
9	38.22534197264219	39.0	39.0	39.0	37.0	39.0
10	38.220302375809936	39.0	39.0	39.0	37.0	39.0
11	38.309575233981285	39.0	39.0	39.0	37.0	39.0
12	38.268178545716346	39.0	39.0	39.0	37.0	39.0
13	38.30417566594672	39.0	39.0	39.0	37.0	39.0
14	39.78113750899928	41.0	40.0	41.0	38.0	41.0
15	39.81857451403888	41.0	40.0	41.0	38.0	41.0
16	39.793016558675305	41.0	40.0	41.0	38.0	41.0
17	39.78041756659467	41.0	40.0	41.0	38.0	41.0
18	39.73542116630669	41.0	40.0	41.0	37.0	41.0
19	39.81965442764579	41.0	40.0	41.0	38.0	41.0
20	39.77429805615551	41.0	40.0	41.0	38.0	41.0
21	39.64002879769618	41.0	40.0	41.0	37.0	41.0
22	39.71238300935925	41.0	40.0	41.0	37.0	41.0
23	39.65802735781138	41.0	40.0	41.0	37.0	41.0
24	39.68142548596112	41.0	40.0	41.0	37.0	41.0
25	39.65334773218142	41.0	40.0	41.0	37.0	41.0
26	39.50359971202304	41.0	39.0	41.0	37.0	41.0
27	39.34809215262779	41.0	39.0	41.0	36.0	41.0
28	39.27177825773938	41.0	39.0	41.0	36.0	41.0
29	39.30885529157668	41.0	39.0	41.0	36.0	41.0
30	39.22894168466523	41.0	39.0	41.0	36.0	41.0
31	39.15658747300216	41.0	39.0	41.0	35.0	41.0
32	39.0655147588193	41.0	39.0	41.0	35.0	41.0
33	38.99928005759539	41.0	39.0	41.0	35.0	41.0
34	38.992080633549314	40.0	39.0	41.0	35.0	41.0
35	38.882649388048954	40.0	39.0	41.0	35.0	41.0
36	38.89020878329734	40.0	39.0	41.0	35.0	41.0
37	38.765298776097914	40.0	38.0	41.0	35.0	41.0
38	38.67458603311735	40.0	38.0	41.0	35.0	41.0
39	38.53095752339813	40.0	38.0	41.0	35.0	41.0
40	38.402447804175665	40.0	38.0	41.0	34.0	41.0
41	38.460763138948884	40.0	38.0	41.0	35.0	41.0
42	38.42512598992081	40.0	38.0	41.0	35.0	41.0
43	37.60223182145428	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	6.0
27	5.0
28	11.0
29	10.0
30	23.0
31	29.0
32	23.0
33	42.0
34	42.0
35	89.0
36	137.0
37	211.0
38	516.0
39	1631.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.37652987760979	15.550755939524837	12.059035277177827	29.013678905687545
2	21.058315334773216	21.058315334773216	30.74154067674586	27.141828653707705
3	23.038156947444204	19.726421886249103	27.96976241900648	29.265658747300215
4	16.70266378689705	14.938804895608351	33.4053275737941	34.953203743700506
5	16.954643628509718	33.621310295176386	32.82937365010799	16.594672426205904
6	35.70914326853852	33.33333333333333	14.002879769618431	16.954643628509718
7	31.641468682505398	26.313894888408928	19.51043916486681	22.534197264218864
8	27.60979121670266	30.849532037437005	19.51043916486681	22.03023758099352
9	27.96976241900648	13.462922966162708	16.558675305975523	42.00863930885529
10	20.19438444924406	23.254139668826493	29.44564434845212	27.10583153347732
11	38.624910007199425	20.050395968322533	18.466522678185747	22.8581713462923
12	22.75017998560115	22.28221742260619	27.68178545716343	27.28581713462923
13	36.89704823614111	16.19870410367171	19.07847372210223	27.825773938084954
14	24.29805615550756	20.19438444924406	23.542116630669547	31.96544276457883
15	29.013678905687545	24.190064794816415	18.934485241180706	27.861771058315334
16	26.241900647948164	22.894168466522675	22.174226061915046	28.68970482361411
17	27.141828653707705	24.15406767458603	20.15838732901368	28.545716342692586
18	26.961843052555796	21.238300935925125	21.634269258459323	30.165586753059753
19	28.149748020158388	21.34629229661627	22.606191504679625	27.897768178545718
20	31.353491720662348	20.734341252699785	21.52627789776818	26.38588912886969
21	27.03383729301656	21.706263498920087	21.958243340532757	29.301655867530595
22	28.437724982001438	21.562275017998562	21.202303815694744	28.797696184305256
23	28.545716342692586	21.238300935925125	22.31821454283657	27.897768178545718
24	27.96976241900648	21.958243340532757	21.31029517638589	28.761699064074875
25	28.437724982001438	21.742260619150468	22.93016558675306	26.88984881209503
26	29.697624190064797	22.354211663066955	21.670266378689703	26.277897768178544
27	26.709863210943123	21.778257739380848	23.542116630669547	27.96976241900648
28	25.70194384449244	23.398128149748022	23.110151187904968	27.789776817854573
29	26.09791216702664	23.8300935925126	21.166306695464364	28.9056875449964
30	27.32181425485961	20.518358531317496	24.190064794816415	27.96976241900648
31	28.401727861771057	22.174226061915046	22.28221742260619	27.141828653707705
32	27.68178545716343	19.51043916486681	24.26205903527718	28.545716342692586
33	26.277897768178544	21.634269258459323	24.73002159827214	27.35781137508999
34	26.169906407487403	20.734341252699785	23.038156947444204	30.057595392368608
35	25.557955363570915	23.614110871130308	24.442044636429085	26.38588912886969
36	26.601871850251978	22.28221742260619	24.658027357811374	26.457883369330453
37	27.03383729301656	21.418286537077034	24.226061915046795	27.32181425485961
38	27.141828653707705	21.202303815694744	25.01799856011519	26.63786897048236
39	25.305975521958246	20.44636429085673	27.10583153347732	27.141828653707705
40	24.98200143988481	21.850251979841612	25.44996400287977	27.717782577393805
41	24.010079193664506	21.52627789776818	27.35781137508999	27.10583153347732
42	23.146148308135352	21.166306695464364	26.997840172786177	28.68970482361411
43	21.34629229661627	22.138228941684666	27.861771058315334	28.65370770338373
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	1.5
7	2.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	2.5
16	4.0
17	3.5
18	3.0
19	3.0
20	5.0
21	7.0
22	5.0
23	3.0
24	4.5
25	6.0
26	6.0
27	6.0
28	6.0
29	7.5
30	9.0
31	19.0
32	29.0
33	29.0
34	27.0
35	25.0
36	25.0
37	25.0
38	37.0
39	49.0
40	49.0
41	61.5
42	74.0
43	97.5
44	121.0
45	152.0
46	183.0
47	183.0
48	212.0
49	241.0
50	259.0
51	277.0
52	299.0
53	321.0
54	321.0
55	313.0
56	305.0
57	286.5
58	268.0
59	247.5
60	227.0
61	227.0
62	196.0
63	165.0
64	147.5
65	130.0
66	115.5
67	101.0
68	101.0
69	85.0
70	69.0
71	53.5
72	38.0
73	41.0
74	44.0
75	44.0
76	28.0
77	12.0
78	13.0
79	14.0
80	11.0
81	8.0
82	8.0
83	7.0
84	6.0
85	3.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2778.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.92080633549317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.43474779823859	84.01727861771057
2	4.363490792634107	7.847372210223182
3	1.200960768614892	3.2397408207343417
4	0.4403522818254604	1.5838732901367891
5	0.24019215372297836	1.079913606911447
6	0.12009607686148918	0.6479481641468683
7	0.08006405124099279	0.5039596832253419
8	0.040032025620496396	0.28797696184305255
9	0.040032025620496396	0.32397408207343414
>10	0.040032025620496396	0.4679625629949604
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	13	0.4679625629949604	TruSeq Adapter, Index 6 (95% over 21bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	9	0.32397408207343414	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	8	0.28797696184305255	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	7	0.25197984161267095	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	7	0.25197984161267095	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	6	0.21598272138228944	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	6	0.21598272138228944	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	6	0.21598272138228944	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	5	0.17998560115190784	RNA PCR Primer, Index 6 (95% over 22bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	5	0.17998560115190784	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	5	0.17998560115190784	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	5	0.17998560115190784	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	5	0.17998560115190784	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	5	0.17998560115190784	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	4	0.14398848092152627	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	4	0.14398848092152627	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	4	0.14398848092152627	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	4	0.14398848092152627	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	4	0.14398848092152627	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	4	0.14398848092152627	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	4	0.14398848092152627	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	4	0.14398848092152627	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.14398848092152627	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	4	0.14398848092152627	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	4	0.14398848092152627	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.10799136069114472	No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT	3	0.10799136069114472	No Hit
CTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA	3	0.10799136069114472	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	3	0.10799136069114472	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	3	0.10799136069114472	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	3	0.10799136069114472	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	3	0.10799136069114472	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	3	0.10799136069114472	No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC	3	0.10799136069114472	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3	0.10799136069114472	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	3	0.10799136069114472	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	3	0.10799136069114472	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	3	0.10799136069114472	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	3	0.10799136069114472	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	3	0.10799136069114472	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	3	0.10799136069114472	No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG	3	0.10799136069114472	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	3	0.10799136069114472	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	3	0.10799136069114472	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	3	0.10799136069114472	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	3	0.10799136069114472	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	3	0.10799136069114472	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	3	0.10799136069114472	No Hit
ATGCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATG	3	0.10799136069114472	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	3	0.10799136069114472	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	3	0.10799136069114472	No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC	3	0.10799136069114472	No Hit
CGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGAT	3	0.10799136069114472	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	3	0.10799136069114472	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTCTG	3	0.10799136069114472	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.1079913606911447	0.0
28	0.0	0.0	0.0	0.14398848092152627	0.0
29	0.0	0.0	0.0	0.28797696184305255	0.0
30	0.0	0.0	0.0	0.4679625629949604	0.0
31	0.0	0.0	0.0	0.8639308855291576	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE