FastQCFastQC Report
Fri 10 Feb 2017
ERR1632675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT130.7291082445316881Illumina PCR Primer Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT80.448681996634885No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA80.448681996634885No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA80.448681996634885No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA60.33651149747616377No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA60.33651149747616377No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA60.33651149747616377No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA60.33651149747616377No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT50.28042624789680315No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC50.28042624789680315No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA50.28042624789680315No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC50.28042624789680315No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC50.28042624789680315No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA50.28042624789680315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40.2243409983174425No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC40.2243409983174425Illumina PCR Primer Index 10 (95% over 22bp)
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC40.2243409983174425No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.2243409983174425No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA40.2243409983174425No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC40.2243409983174425No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA40.2243409983174425No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC40.2243409983174425No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT40.2243409983174425No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC40.2243409983174425No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG40.2243409983174425No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT40.2243409983174425No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC40.2243409983174425No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT30.16825574873808188No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30.16825574873808188No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC30.16825574873808188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30.16825574873808188No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT30.16825574873808188No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG30.16825574873808188No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG30.16825574873808188No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC30.16825574873808188No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA30.16825574873808188No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCC30.16825574873808188No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA30.16825574873808188No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA30.16825574873808188No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA30.16825574873808188No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC30.16825574873808188No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC30.16825574873808188No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGC30.16825574873808188No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA30.16825574873808188No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA20.11217049915872125No Hit
GCGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTC20.11217049915872125No Hit
CCTCAGCCTTGATTTTCCGGATTTGCCTGGAAAACCAGCCCTG20.11217049915872125No Hit
CCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGC20.11217049915872125No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGCTGT20.11217049915872125No Hit
AGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAG20.11217049915872125No Hit
CCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACAC20.11217049915872125No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG20.11217049915872125No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT20.11217049915872125No Hit
GGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGC20.11217049915872125No Hit
CTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAC20.11217049915872125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20.11217049915872125No Hit
TGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCC20.11217049915872125No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCACTG20.11217049915872125No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT20.11217049915872125No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA20.11217049915872125No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG20.11217049915872125No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTCTGTC20.11217049915872125No Hit
TGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATC20.11217049915872125No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG20.11217049915872125No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG20.11217049915872125No Hit
CTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCC20.11217049915872125No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGCTGT20.11217049915872125No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA20.11217049915872125No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT20.11217049915872125No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT20.11217049915872125No Hit
TATCAACGCAGAGTACGGGCACAGGTGGTCAGGTAGAGAACTG20.11217049915872125No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG20.11217049915872125No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC20.11217049915872125No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA20.11217049915872125No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC20.11217049915872125No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG20.11217049915872125No Hit
ATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTA20.11217049915872125No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA20.11217049915872125No Hit
CCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGT20.11217049915872125No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA20.11217049915872125No Hit
GCTTTTTACGAGGCCAACCGCCCCTCGGCATGCACCTTGGGCT20.11217049915872125No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT20.11217049915872125No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTG20.11217049915872125No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACC20.11217049915872125No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAG20.11217049915872125No Hit
CAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAA20.11217049915872125No Hit
GGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTAC20.11217049915872125No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTC20.11217049915872125No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC20.11217049915872125No Hit
TCAACGCAGAGTACGGGCAGTACCGTGAGGAAAAAAAACTGTC20.11217049915872125No Hit
CGGCATGACCTACCGGCCGAACGAGAGCTGGGCCTACACCTGT20.11217049915872125No Hit
GCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAG20.11217049915872125No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG20.11217049915872125No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC20.11217049915872125No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC20.11217049915872125No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT20.11217049915872125No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC20.11217049915872125No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC20.11217049915872125No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT20.11217049915872125No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA20.11217049915872125No Hit
ACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCTGT20.11217049915872125No Hit
ACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAA20.11217049915872125No Hit
GCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTT20.11217049915872125No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC20.11217049915872125No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT20.11217049915872125No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20.11217049915872125No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC20.11217049915872125No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT20.11217049915872125No Hit
TCACCGCGCGCGATTTCATGAAGGCCGCGATCCCGATCCCTGT20.11217049915872125No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC20.11217049915872125No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGCTGTC20.11217049915872125No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT20.11217049915872125No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA20.11217049915872125No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA20.11217049915872125No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTCTG20.11217049915872125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)