FastQCFastQC Report
Fri 10 Feb 2017
ERR1632673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16558
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT590.3563232274429279RNA PCR Primer, Index 41 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA510.3080082135523614No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA370.22345693924387006No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA350.21137818577122838No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA340.20533880903490762No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC330.19929943229858677No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA290.17514192535330353No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA270.1630631718806619No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT260.1570237951443411No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT260.1570237951443411No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT250.1509844184080203No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC230.13890566493537868No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC230.13890566493537868No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA230.13890566493537868No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC220.13286628819905785RNA PCR Primer, Index 41 (95% over 23bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT210.12682691146273703No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG210.12682691146273703No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC210.12682691146273703No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC200.12078753472641622No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT190.11474815799009541No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA190.11474815799009541No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC190.11474815799009541No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA190.11474815799009541No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT180.10870878125377462No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC170.10266940451745381No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC170.10266940451745381No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC170.10266940451745381No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA170.10266940451745381No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA170.10266940451745381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCC251.1818537E-437.09
AACTCCG251.1818537E-437.05
TAACTCC251.1818537E-437.04
CTAACTC251.1818537E-437.03
GCTAACT251.1818537E-437.02
GGCTAAC251.1818537E-437.01
TACGGAG352.2502392E-531.71428530
ATACGGA303.4498083E-430.83333429
CGTGCCA303.4498083E-430.83333410
TAATACG303.4498083E-430.83333427
TCCGTGC303.4498083E-430.8333348
AATACGG303.4498083E-430.83333428
GTAATAC303.4498083E-430.83333426
CTCCGTG303.4498083E-430.8333347
GGTAATA303.4498083E-430.83333425
ACTCCGT405.5994038E-527.756
CAGCAGC358.5040054E-426.4285715
GCGGTAA358.5040054E-426.4285723
CGCGGTA358.5040054E-426.4285722
AGCCGCG358.5040054E-426.4285719
Produced by FastQC (version 0.11.5)