##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632673.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16558
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04312114989733	34.0	33.0	34.0	31.0	34.0
2	33.17628940693321	34.0	33.0	34.0	31.0	34.0
3	33.2462253895398	34.0	34.0	34.0	31.0	34.0
4	36.51872206788259	37.0	37.0	37.0	35.0	37.0
5	36.49571204251721	37.0	37.0	37.0	35.0	37.0
6	36.534907597535934	37.0	37.0	37.0	35.0	37.0
7	36.53859161734509	37.0	37.0	37.0	35.0	37.0
8	36.50924024640657	37.0	37.0	37.0	35.0	37.0
9	38.31235656480251	39.0	39.0	39.0	37.0	39.0
10	38.34315738615775	39.0	39.0	39.0	37.0	39.0
11	38.41852880782703	39.0	39.0	39.0	37.0	39.0
12	38.38724483633289	39.0	39.0	39.0	37.0	39.0
13	38.4149655755526	39.0	39.0	39.0	37.0	39.0
14	39.98900833433989	41.0	40.0	41.0	38.0	41.0
15	39.96406570841889	41.0	40.0	41.0	38.0	41.0
16	39.92227322140355	41.0	40.0	41.0	38.0	41.0
17	39.89400893827757	41.0	40.0	41.0	38.0	41.0
18	39.90886580504892	41.0	40.0	41.0	38.0	41.0
19	39.91979707694166	41.0	40.0	41.0	38.0	41.0
20	39.9220316463341	41.0	40.0	41.0	38.0	41.0
21	39.87619277690543	41.0	40.0	41.0	38.0	41.0
22	39.85855779683536	41.0	40.0	41.0	38.0	41.0
23	39.77926078028747	41.0	40.0	41.0	38.0	41.0
24	39.777750936103395	41.0	40.0	41.0	38.0	41.0
25	39.7258726899384	41.0	40.0	41.0	37.0	41.0
26	39.63703345814712	41.0	40.0	41.0	37.0	41.0
27	39.58122961710352	41.0	40.0	41.0	37.0	41.0
28	39.509723396545475	41.0	40.0	41.0	37.0	41.0
29	39.45023553569272	41.0	40.0	41.0	37.0	41.0
30	39.40518178523976	41.0	39.0	41.0	37.0	41.0
31	39.35559850223457	41.0	39.0	41.0	36.0	41.0
32	39.298828360913156	41.0	39.0	41.0	36.0	41.0
33	39.242903732334824	41.0	39.0	41.0	36.0	41.0
34	39.173088537262956	41.0	39.0	41.0	35.0	41.0
35	39.1309940814108	41.0	39.0	41.0	35.0	41.0
36	39.07700205338809	41.0	39.0	41.0	35.0	41.0
37	39.024157506945286	41.0	39.0	41.0	35.0	41.0
38	38.94407537142167	40.0	39.0	41.0	35.0	41.0
39	38.85118975721706	40.0	39.0	41.0	35.0	41.0
40	38.77134919676289	40.0	38.0	41.0	35.0	41.0
41	38.75111728469622	40.0	38.0	41.0	35.0	41.0
42	38.66149293392922	40.0	38.0	41.0	35.0	41.0
43	37.926681966421064	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	5.0
22	3.0
23	6.0
24	9.0
25	13.0
26	20.0
27	31.0
28	41.0
29	59.0
30	63.0
31	101.0
32	136.0
33	148.0
34	271.0
35	407.0
36	633.0
37	1259.0
38	3215.0
39	10136.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.040101461529176	16.23988404396666	13.461770745259088	28.258243749245075
2	21.705519990336995	19.458871844425655	31.984539195555016	26.85106896968233
3	21.391472400048315	19.57362000241575	28.95881145065829	30.07609614687764
4	15.817127672424206	15.593670733180337	33.59705278415267	34.99214881024278
5	17.610822563111487	32.46164995772436	32.34690179973427	17.580625679429883
6	34.90155815919797	33.13202077545597	15.291701896364296	16.67471916898176
7	29.88283609131538	27.183234690179976	19.984297620485567	22.949631598019085
8	28.197849981881866	29.514434110399808	20.17151829931151	22.11619760840681
9	28.517936948906875	12.646454885855778	17.429641261021864	41.40596690421548
10	19.72460442082377	22.599347747312475	29.230583403792725	28.44546442807102
11	39.22575190240367	19.41055683053509	18.709989129121873	22.653702137939362
12	23.04626162580022	23.43882111366107	27.09264403913516	26.422273221403554
13	35.97052784152675	17.163908684623745	19.80311631839594	27.062447155453555
14	25.226476627612033	18.963642952047348	22.303418287232756	33.50646213310786
15	29.89491484478802	23.783065587631356	19.295808672544993	27.026210895035636
16	26.156540645005432	23.758908080686073	22.32153641744172	27.763014856866768
17	26.941659620727144	23.36634859282522	21.542456818456333	28.1495349679913
18	27.014132141562992	20.594274670853967	23.05834037927286	29.333252808310185
19	28.385070660707818	22.122236985143136	22.66578089141201	26.826911462737048
20	30.68003382050972	20.376857108346417	21.80215001811813	27.140959053025725
21	27.605991061722428	22.073921971252567	22.454402705640778	27.865684261384228
22	28.64476386036961	22.55103273342191	20.99891291218746	27.80529049402102
23	28.681000120787537	21.8504650320087	21.58473245561058	27.88380239159319
24	28.203889358618188	21.705519990336995	21.89878004589926	28.19181060514555
25	28.18577122840923	22.4906389660587	22.092040101461528	27.23154970407054
26	28.427346297862062	22.152433868824737	21.627008092764825	27.793211740548372
27	26.663848290856386	22.14639449208842	23.795144341104	27.394612875951204
28	26.63365140717478	22.97982848170069	21.71759874380964	28.668921367314894
29	27.907959898538472	22.99794661190965	22.345693924387003	26.748399565164878
30	27.03225027177195	21.753835004227565	23.336151709143614	27.877763014856864
31	28.10121995410074	21.86254378548134	22.67785964488465	27.358376615533274
32	26.192776905423358	21.959173813262474	22.671820268148327	29.176229013165838
33	26.283367556468175	21.331078632685106	24.501751419253534	27.88380239159319
34	26.156540645005432	22.41816644522285	23.281797318516727	28.14349559125498
35	26.361879454040345	22.641623384466723	24.139388815074284	26.857108346418652
36	26.301485686677132	21.524338688247376	25.075492209204008	27.098683415871484
37	26.91146273704554	21.306921125739827	24.211861335910136	27.56975480130451
38	26.047831863751657	20.787534726416233	25.818335547771472	27.346297862060638
39	25.570721101582315	20.32250271771953	26.494745742239402	27.612030438458753
40	24.912429037323346	21.053267302814348	27.63014856866771	26.404155091194585
41	24.10919193139268	20.485565889600192	27.418770382896483	27.98647179611064
42	21.856504408745018	21.010991665660104	29.351370938519146	27.781132987075736
43	21.21029109795869	21.349196762894067	28.850102669404514	28.590409469742724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	2.0
10	2.0
11	2.0
12	2.0
13	3.5
14	5.0
15	11.0
16	17.0
17	15.0
18	13.0
19	13.0
20	19.5
21	26.0
22	27.5
23	29.0
24	29.5
25	30.0
26	30.0
27	36.5
28	43.0
29	59.5
30	76.0
31	90.5
32	105.0
33	105.0
34	142.5
35	180.0
36	205.0
37	230.0
38	270.0
39	310.0
40	310.0
41	401.0
42	492.0
43	587.5
44	683.0
45	909.0
46	1135.0
47	1135.0
48	1291.0
49	1447.0
50	1591.0
51	1735.0
52	1788.5
53	1842.0
54	1842.0
55	1802.5
56	1763.0
57	1697.0
58	1631.0
59	1487.5
60	1344.0
61	1344.0
62	1243.0
63	1142.0
64	932.0
65	722.0
66	643.5
67	565.0
68	565.0
69	452.0
70	339.0
71	306.0
72	273.0
73	202.0
74	131.0
75	131.0
76	109.5
77	88.0
78	75.5
79	63.0
80	48.0
81	33.0
82	33.0
83	31.5
84	30.0
85	20.5
86	11.0
87	11.5
88	12.0
89	12.0
90	7.5
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	16558.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.16765309820026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.42725797728501	70.68486532189878
2	5.045198176620567	7.887426017634979
3	1.885188905199722	4.420823770986834
4	0.7726184037703778	2.4157506945283247
5	0.4944757784130418	1.9326005556226595
6	0.3090473615081511	1.4494504167169948
7	0.11589276056555667	0.6341345573136852
8	0.1777022328671869	1.1112453194830294
9	0.10044039249014912	0.706607078149535
>10	0.6567256432048211	8.092764826669887
>50	0.015452368075407556	0.6643314409952893
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	59	0.3563232274429279	RNA PCR Primer, Index 41 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	51	0.3080082135523614	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	37	0.22345693924387006	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	35	0.21137818577122838	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	34	0.20533880903490762	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	33	0.19929943229858677	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	29	0.17514192535330353	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	27	0.1630631718806619	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	26	0.1570237951443411	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	26	0.1570237951443411	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	25	0.1509844184080203	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	23	0.13890566493537868	No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	23	0.13890566493537868	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	23	0.13890566493537868	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	22	0.13286628819905785	RNA PCR Primer, Index 41 (95% over 23bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	21	0.12682691146273703	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	21	0.12682691146273703	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	21	0.12682691146273703	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	20	0.12078753472641622	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	19	0.11474815799009541	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	19	0.11474815799009541	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	19	0.11474815799009541	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	19	0.11474815799009541	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	18	0.10870878125377462	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	17	0.10266940451745381	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	17	0.10266940451745381	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	17	0.10266940451745381	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	17	0.10266940451745381	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	17	0.10266940451745381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.03019688368160406	0.0
28	0.0	0.0	0.0	0.09059065104481218	0.0
29	0.0	0.0	0.0	0.21741756250754923	0.0
30	0.0	0.0	0.0	0.39255948786085276	0.0
31	0.0	0.0	0.0	0.8998671337118009	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCC	25	1.1818537E-4	37.0	9
AACTCCG	25	1.1818537E-4	37.0	5
TAACTCC	25	1.1818537E-4	37.0	4
CTAACTC	25	1.1818537E-4	37.0	3
GCTAACT	25	1.1818537E-4	37.0	2
GGCTAAC	25	1.1818537E-4	37.0	1
TACGGAG	35	2.2502392E-5	31.714285	30
ATACGGA	30	3.4498083E-4	30.833334	29
CGTGCCA	30	3.4498083E-4	30.833334	10
TAATACG	30	3.4498083E-4	30.833334	27
TCCGTGC	30	3.4498083E-4	30.833334	8
AATACGG	30	3.4498083E-4	30.833334	28
GTAATAC	30	3.4498083E-4	30.833334	26
CTCCGTG	30	3.4498083E-4	30.833334	7
GGTAATA	30	3.4498083E-4	30.833334	25
ACTCCGT	40	5.5994038E-5	27.75	6
CAGCAGC	35	8.5040054E-4	26.42857	15
GCGGTAA	35	8.5040054E-4	26.42857	23
CGCGGTA	35	8.5040054E-4	26.42857	22
AGCCGCG	35	8.5040054E-4	26.42857	19
>>END_MODULE