FastQCFastQC Report
Fri 10 Feb 2017
ERR1632671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27888
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT940.33706253585771656TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA560.2008032128514056No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA470.16853126792885828No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA470.16853126792885828No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA380.13625932300631097No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA360.12908777969018934No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC340.1219162363740677No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC340.1219162363740677No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT330.11833046471600689No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA310.11115892139988526No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA280.1004016064257028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC250.005405885629.59999837
ACCTGTC358.6530816E-426.42857236
CCTGTCT1203.0793366E-513.87500137
CTGTCTC2203.4015102E-912.61363637
GCTGTCT1150.00500455611.2608736
Produced by FastQC (version 0.11.5)