FastQCFastQC Report
Fri 10 Feb 2017
ERR1632669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19890
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA570.2865761689291101No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT340.17094017094017094TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA330.16591251885369532No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA330.16591251885369532No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA290.14580191050779287No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC260.130718954248366No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA250.1256913021618904No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC240.12066365007541478No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA240.12066365007541478No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG220.11060834590246355No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT200.10055304172951231No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC200.10055304172951231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTG200.001798709637.07
ATCTTTA251.1904731E-436.9999964
CATGAAG303.4747305E-430.83333416
GTCATCT303.4747305E-430.8333341
GAAGTGC303.4747305E-430.83333419
ATGAAGT303.4747305E-430.83333417
TGAAGTG303.4747305E-430.83333418
GACCATG303.4747305E-430.83333413
TCCGTGC250.00536881429.5999988
CTTTAGT358.5648615E-426.428576
TGACCAT358.5648615E-426.4285712
CATCTTT358.5648615E-426.428573
AGTGCGA358.5648615E-426.4285721
TTTAGTG358.5648615E-426.428577
AAGTGCG358.5648615E-426.4285720
TCTTTAG358.5648615E-426.428575
TTAGTGA358.5648615E-426.428578
TAGTGAC400.001865497323.1250029
ACCATGA400.001865497323.12500214
TGCGATT400.001865497323.12500223
Produced by FastQC (version 0.11.5)