##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632669.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19890
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04509803921569	34.0	33.0	34.0	31.0	34.0
2	33.17551533433886	34.0	33.0	34.0	31.0	34.0
3	33.26184012066365	34.0	34.0	34.0	31.0	34.0
4	36.54409250879839	37.0	37.0	37.0	35.0	37.0
5	36.490045248868775	37.0	37.0	37.0	35.0	37.0
6	36.54610356963298	37.0	37.0	37.0	35.0	37.0
7	36.534489693313226	37.0	37.0	37.0	35.0	37.0
8	36.50678733031674	37.0	37.0	37.0	35.0	37.0
9	38.30196078431373	39.0	39.0	39.0	37.0	39.0
10	38.35967823026647	39.0	39.0	39.0	37.0	39.0
11	38.416339869281046	39.0	39.0	39.0	37.0	39.0
12	38.35696329813977	39.0	39.0	39.0	37.0	39.0
13	38.40950226244344	39.0	39.0	39.0	37.0	39.0
14	39.96706887883359	41.0	40.0	41.0	38.0	41.0
15	39.942735042735045	41.0	40.0	41.0	38.0	41.0
16	39.94977375565611	41.0	40.0	41.0	38.0	41.0
17	39.912518853695325	41.0	40.0	41.0	38.0	41.0
18	39.91216691804927	41.0	40.0	41.0	38.0	41.0
19	39.89441930618401	41.0	40.0	41.0	38.0	41.0
20	39.87833081950729	41.0	40.0	41.0	38.0	41.0
21	39.839366515837106	41.0	40.0	41.0	38.0	41.0
22	39.81920563097034	41.0	40.0	41.0	38.0	41.0
23	39.76752136752137	41.0	40.0	41.0	38.0	41.0
24	39.7684766214178	41.0	40.0	41.0	38.0	41.0
25	39.72347913524384	41.0	40.0	41.0	37.0	41.0
26	39.615686274509805	41.0	40.0	41.0	37.0	41.0
27	39.546254399195576	41.0	40.0	41.0	37.0	41.0
28	39.51116138763198	41.0	40.0	41.0	37.0	41.0
29	39.4684766214178	41.0	40.0	41.0	37.0	41.0
30	39.41458019105078	41.0	39.0	41.0	36.0	41.0
31	39.36465560583208	41.0	39.0	41.0	36.0	41.0
32	39.30336852689794	41.0	39.0	41.0	36.0	41.0
33	39.24328808446455	41.0	39.0	41.0	36.0	41.0
34	39.1982905982906	41.0	39.0	41.0	35.0	41.0
35	39.09602815485169	41.0	39.0	41.0	35.0	41.0
36	39.07435897435897	41.0	39.0	41.0	35.0	41.0
37	39.01357466063349	41.0	39.0	41.0	35.0	41.0
38	38.96425339366516	40.0	39.0	41.0	35.0	41.0
39	38.84117647058824	40.0	38.0	41.0	35.0	41.0
40	38.74454499748617	40.0	38.0	41.0	35.0	41.0
41	38.69904474610357	40.0	38.0	41.0	35.0	41.0
42	38.663197586727	40.0	38.0	41.0	35.0	41.0
43	37.89552538964303	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	4.0
21	1.0
22	4.0
23	8.0
24	9.0
25	16.0
26	20.0
27	32.0
28	54.0
29	55.0
30	76.0
31	118.0
32	146.0
33	190.0
34	343.0
35	533.0
36	774.0
37	1578.0
38	3837.0
39	12090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.539467068878835	16.03318250377074	11.860231271995977	28.567119155354447
2	21.97083961789844	18.366013071895427	30.819507290095526	28.843640020110605
3	21.196581196581196	19.386626445449977	27.712418300653596	31.70437405731523
4	15.746606334841628	14.539969834087483	32.71493212669683	36.99849170437406
5	17.702362996480645	32.584213172448464	32.85570638511815	16.85771744595274
6	36.80241327300151	32.845651080945196	14.303670186023126	16.048265460030166
7	31.36249371543489	26.450477626948217	19.376571141277026	22.81045751633987
8	28.783308195072898	30.165912518853695	19.06988436400201	21.980894922071393
9	28.169934640522875	13.031674208144798	16.80241327300151	41.99597787833082
10	19.522373051784818	23.202614379084967	29.41679235796883	27.858220211161388
11	41.11111111111111	18.541980894922073	17.82302664655606	22.52388134741076
12	24.233283056812468	22.785319255907492	25.656108597285066	27.32528908999497
13	35.7114127702363	16.611362493715433	19.808949220713927	27.86827551533434
14	24.479638009049772	18.260432378079436	21.794871794871796	35.465057817998996
15	29.250879839115136	23.795877325289087	18.994469582704877	27.9587732528909
16	27.340372046254398	22.74007038712921	20.8044243338361	29.115133232780295
17	28.456510809451984	22.85570638511815	20.633484162895925	28.054298642533936
18	27.893413775766717	21.06586224233283	22.13172448466566	28.908999497234795
19	28.687782805429862	22.227249874308697	22.187028657616892	26.897938662644545
20	29.768728004022122	21.07591754650578	22.116641528406234	27.038712921065862
21	28.54198089492207	21.784816490698844	20.678733031674206	28.99446958270488
22	29.069884364002014	21.84514831573655	20.46757164404223	28.617395676219203
23	29.125188536953246	21.468074409250878	21.15635997988939	28.250377073906485
24	28.969331322272502	21.060834590246355	21.045751633986928	28.924082453494222
25	28.868778280542983	21.236802413273	22.101558572146807	27.792860734037205
26	28.99446958270488	21.749622926093515	21.32730015082956	27.928607340372047
27	27.812971342383108	21.085972850678733	22.664655605832078	28.436400201106082
28	27.00854700854701	21.86023127199598	21.603821015585723	29.527400703871294
29	28.25540472599296	22.292609351432883	21.543489190548016	27.908496732026144
30	27.898441427853193	21.598793363499247	22.709904474610358	27.792860734037205
31	28.28054298642534	21.287078934137757	21.6289592760181	28.8034188034188
32	26.752136752136753	21.66415284062343	21.804927099044747	29.778783308195074
33	26.877828054298643	20.81447963800905	23.43891402714932	28.868778280542983
34	27.712418300653596	20.910005027652087	23.17747611865259	28.20010055304173
35	26.52589240824535	22.4333836098542	23.162393162393162	27.87833081950729
36	27.40070387129211	21.085972850678733	23.57466063348416	27.938662644544998
37	26.60633484162896	20.6184012066365	24.132730015082956	28.64253393665158
38	26.0834590246355	19.90950226244344	25.072900955253896	28.934137757667173
39	25.58069381598793	19.798893916540976	26.078431372549023	28.54198089492207
40	24.69079939668175	20.55806938159879	27.219708396178984	27.531422825540474
41	23.64504776269482	19.431875314228257	27.767722473604827	29.1553544494721
42	22.23730517848165	20.643539467068877	28.969331322272502	28.149824032176973
43	21.659125188536954	20.794369029663148	28.41628959276018	29.13021618903972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	0.5
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	4.0
15	7.0
16	10.0
17	8.0
18	6.0
19	6.0
20	9.0
21	12.0
22	10.0
23	8.0
24	12.0
25	16.0
26	16.0
27	21.5
28	27.0
29	47.5
30	68.0
31	84.0
32	100.0
33	100.0
34	138.5
35	177.0
36	230.0
37	283.0
38	348.0
39	413.0
40	413.0
41	512.0
42	611.0
43	704.0
44	797.0
45	1008.0
46	1219.0
47	1219.0
48	1370.0
49	1521.0
50	1698.5
51	1876.0
52	1986.0
53	2096.0
54	2096.0
55	2038.5
56	1981.0
57	1956.5
58	1932.0
59	1814.5
60	1697.0
61	1697.0
62	1589.5
63	1482.0
64	1255.5
65	1029.0
66	929.5
67	830.0
68	830.0
69	718.5
70	607.0
71	527.5
72	448.0
73	349.0
74	250.0
75	250.0
76	200.0
77	150.0
78	123.0
79	96.0
80	86.5
81	77.0
82	77.0
83	51.0
84	25.0
85	23.0
86	21.0
87	15.5
88	10.0
89	10.0
90	8.5
91	7.0
92	4.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	19890.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.68476621417797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.34744010701691	75.53041729512317
2	5.138027483886659	8.49673202614379
3	1.4532409096436822	3.604826546003016
4	0.6566946369937978	2.171945701357466
5	0.370910859783534	1.5334338863750627
6	0.3465888361911711	1.7194570135746607
7	0.13377112975799585	0.7742584213172449
8	0.08512708257327009	0.563097033685269
9	0.11552961206372371	0.8597285067873304
>10	0.3465888361911711	4.459527400703871
>50	0.0060805058980907215	0.2865761689291101
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	57	0.2865761689291101	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	34	0.17094017094017094	TruSeq Adapter, Index 7 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	33	0.16591251885369532	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	33	0.16591251885369532	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	29	0.14580191050779287	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	26	0.130718954248366	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	25	0.1256913021618904	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	24	0.12066365007541478	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	24	0.12066365007541478	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	22	0.11060834590246355	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	20	0.10055304172951231	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	20	0.10055304172951231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.020110608345902465	0.0
28	0.0	0.0	0.0	0.09049773755656108	0.0
29	0.0	0.0	0.0	0.2513826043237808	0.0
30	0.0	0.0	0.0	0.38712921065862244	0.0
31	0.0	0.0	0.0	0.8345902463549523	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTG	20	0.0017987096	37.0	7
ATCTTTA	25	1.1904731E-4	36.999996	4
CATGAAG	30	3.4747305E-4	30.833334	16
GTCATCT	30	3.4747305E-4	30.833334	1
GAAGTGC	30	3.4747305E-4	30.833334	19
ATGAAGT	30	3.4747305E-4	30.833334	17
TGAAGTG	30	3.4747305E-4	30.833334	18
GACCATG	30	3.4747305E-4	30.833334	13
TCCGTGC	25	0.005368814	29.599998	8
CTTTAGT	35	8.5648615E-4	26.42857	6
TGACCAT	35	8.5648615E-4	26.42857	12
CATCTTT	35	8.5648615E-4	26.42857	3
AGTGCGA	35	8.5648615E-4	26.42857	21
TTTAGTG	35	8.5648615E-4	26.42857	7
AAGTGCG	35	8.5648615E-4	26.42857	20
TCTTTAG	35	8.5648615E-4	26.42857	5
TTAGTGA	35	8.5648615E-4	26.42857	8
TAGTGAC	40	0.0018654973	23.125002	9
ACCATGA	40	0.0018654973	23.125002	14
TGCGATT	40	0.0018654973	23.125002	23
>>END_MODULE