FastQCFastQC Report
Fri 10 Feb 2017
ERR1632667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234056
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5930.25335817069419286No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4120.17602625012817444No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3300.14099189937450868No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT3010.12860170215674882RNA PCR Primer, Index 47 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2990.1277472057969033No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2760.11792049765867998No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2530.10809378952045665No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2430.1038213077212291No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2420.10339405954130636No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2380.10168506682161534No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2380.10168506682161534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACA200.00183894337.029
TCTATAC200.00183894337.03
GGTATCA2000.032.3749961
AGTATTA303.5908018E-430.83333415
AAATCCG250.00548751529.59999830
CTACCAC250.00548751529.59999828
GGTCTAT250.00548751529.5999981
TATACCC250.00548751529.5999985
ATACGGC556.2260006E-726.9090929
ATCTAAT358.848221E-426.428571
GCGGTAA2750.023.54545623
AGATTTC400.001926620123.12521
CAGTATT400.001926620123.12514
TAATGTC400.001926620123.1254
TCGCCAT652.6677171E-622.76923413
TAATACG2600.022.76923427
AATACGG2600.022.76923428
ATGCCGT753.7144855E-722.234
CCACGCT502.6932033E-422.19999920
GTAATAC2850.022.07017526
Produced by FastQC (version 0.11.5)