##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632667.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234056
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53027053354753	34.0	31.0	34.0	31.0	34.0
2	32.701579963769355	34.0	31.0	34.0	31.0	34.0
3	32.785038623235465	34.0	31.0	34.0	31.0	34.0
4	36.28978535051441	37.0	37.0	37.0	35.0	37.0
5	36.09320419045015	37.0	35.0	37.0	35.0	37.0
6	36.09627610486379	37.0	35.0	37.0	35.0	37.0
7	36.09205916532795	37.0	35.0	37.0	35.0	37.0
8	36.06155364528147	37.0	35.0	37.0	35.0	37.0
9	37.747987661072564	39.0	37.0	39.0	35.0	39.0
10	37.70168677581434	39.0	37.0	39.0	35.0	39.0
11	37.79778343644256	39.0	37.0	39.0	35.0	39.0
12	37.73712701233893	39.0	37.0	39.0	35.0	39.0
13	37.78385941825888	39.0	37.0	39.0	35.0	39.0
14	39.144704686058034	40.0	38.0	41.0	36.0	41.0
15	39.11495539529002	40.0	38.0	41.0	36.0	41.0
16	39.07585364186349	40.0	38.0	41.0	36.0	41.0
17	39.06077605359401	40.0	38.0	41.0	36.0	41.0
18	39.04628806781283	40.0	38.0	41.0	36.0	41.0
19	39.049001093755344	40.0	38.0	41.0	36.0	41.0
20	39.01476569709813	40.0	38.0	41.0	36.0	41.0
21	38.97829151997812	40.0	38.0	41.0	36.0	41.0
22	38.93460539358102	40.0	38.0	41.0	36.0	41.0
23	38.88947089585398	40.0	38.0	41.0	36.0	41.0
24	38.87300475099976	40.0	38.0	41.0	35.0	41.0
25	38.813873602898454	40.0	38.0	41.0	35.0	41.0
26	38.69824315548416	40.0	38.0	41.0	35.0	41.0
27	38.585667532556315	40.0	38.0	41.0	35.0	41.0
28	38.54292989711864	40.0	38.0	41.0	35.0	41.0
29	38.477855726834605	40.0	38.0	41.0	35.0	41.0
30	38.438677068735686	40.0	38.0	41.0	35.0	41.0
31	38.373645623269645	40.0	38.0	41.0	34.0	41.0
32	38.304871483747476	40.0	38.0	41.0	34.0	41.0
33	38.23318778412004	40.0	38.0	41.0	34.0	41.0
34	38.18492155723417	40.0	38.0	41.0	34.0	41.0
35	38.08559490036573	40.0	38.0	41.0	34.0	41.0
36	38.04060993950166	40.0	38.0	41.0	34.0	41.0
37	37.97672351915781	40.0	37.0	41.0	33.0	41.0
38	37.89528147110093	40.0	37.0	41.0	33.0	41.0
39	37.79912072324572	40.0	37.0	41.0	33.0	41.0
40	37.71740523635369	40.0	37.0	41.0	33.0	41.0
41	37.65567214683666	40.0	37.0	41.0	33.0	41.0
42	37.58365519362888	40.0	36.0	41.0	33.0	41.0
43	36.745889872509146	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	0.0
15	1.0
16	2.0
17	3.0
18	6.0
19	9.0
20	30.0
21	55.0
22	99.0
23	143.0
24	256.0
25	350.0
26	461.0
27	719.0
28	926.0
29	1352.0
30	1773.0
31	2474.0
32	3218.0
33	4593.0
34	6698.0
35	10852.0
36	18399.0
37	36318.0
38	74248.0
39	71068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36355402125987	16.32045322486926	12.411132378576067	27.9048603752948
2	21.636702327648084	19.532077793348602	31.534760911918514	27.296458967084803
3	21.374799193355436	19.579929589499947	28.685015551833747	30.360255665310866
4	16.322162217588954	15.376234747239979	32.518713470280616	35.78288956489045
5	17.33644939672557	33.17838466008135	32.77805311549373	16.707112827699355
6	36.346002666028646	33.062600403322286	14.332894691868614	16.258502238780462
7	30.894743138394233	27.47803944355197	19.155244898656733	22.471972519397067
8	27.911696346173564	31.247650135010424	19.423984003828142	21.416669514987866
9	27.622876576545785	12.88922309190963	16.91133745770243	42.57656287384216
10	19.199251461188776	23.7024472775746	29.59505417506921	27.503247086167416
11	40.25361451960215	18.978364152168712	18.271268414396555	22.496752913832587
12	23.47173326041631	23.421317975185428	25.82715247633045	27.279796288067814
13	35.25523806268585	17.222374132686195	20.578408585979425	26.94397921864853
14	24.76544074922241	18.843780975493043	21.84904467306969	34.541733602214855
15	29.560019824315546	24.006220733499674	19.29794579075093	27.135813651433843
16	27.019602146494854	23.30553371842636	21.479902245616433	28.19496188946235
17	27.848463615545	23.212820863383122	21.003947773182485	27.934767747889396
18	26.8965546706771	21.306012236387872	22.825734012373108	28.97169908056192
19	29.029804833031413	22.200242676966194	22.16179034077315	26.60816214922924
20	29.824913695867654	20.67411217828212	22.347216050859622	27.153758074990602
21	27.788648870355814	21.974655637966983	21.18766449054927	29.049031001127933
22	28.4662644837133	22.28312882387121	20.864237618347744	28.386369074067748
23	28.355607205113305	22.22545031958164	21.490583450114503	27.928359025190552
24	28.70381447175035	21.444440646682843	21.59739549509519	28.25434938647161
25	29.30580715726151	21.692244591038044	21.840072461291314	27.161875790409134
26	28.893939911815973	22.140000683597087	21.44828588030215	27.517773524284788
27	27.74549680418361	21.66532795570291	22.908192911098197	27.68098232901528
28	27.002084971118023	22.34208907270055	21.83494548313224	28.820880473049186
29	27.680127832655433	23.044057832313634	21.751632088047305	27.52418224698363
30	27.359264449533445	22.2040879105855	22.84496018046963	27.591687459411425
31	28.323990839799023	21.966965170728372	22.160935844413302	27.548108145059302
32	26.015141675496462	22.10325734012373	22.542041220904398	29.33955976347541
33	26.867501794442354	21.486310968315276	23.46361554499778	28.18257169224459
34	27.08710735892265	21.656782992104453	23.171377789930613	28.08473185904228
35	26.52698499504392	22.696277813856515	23.66527668592132	27.111460505178247
36	26.69617527429333	21.647810780326076	24.232662268858736	27.423351676521857
37	27.388317325768192	20.870646341046587	23.818231534333663	27.92280479885156
38	26.12494445773661	20.95609597703114	25.37426940561233	27.544690159619922
39	25.670352394298803	19.832006015654375	26.153570085791433	28.344071504255393
40	25.084595139624703	20.51261236627132	27.529736473322625	26.87305602078135
41	23.616997641590046	20.11398981440339	28.09114058174112	28.17787196226544
42	22.69285982841713	20.581399323238884	28.993061489558052	27.732679358785933
43	21.887069761082817	20.9065351881601	28.73585808524456	28.470536965512526
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.0
4	2.0
5	2.0
6	4.5
7	7.0
8	9.5
9	12.0
10	14.0
11	16.0
12	16.0
13	31.5
14	47.0
15	84.5
16	122.0
17	123.5
18	125.0
19	125.0
20	149.0
21	173.0
22	183.0
23	193.0
24	238.0
25	283.0
26	283.0
27	367.5
28	452.0
29	788.5
30	1125.0
31	1303.0
32	1481.0
33	1481.0
34	1945.0
35	2409.0
36	2810.0
37	3211.0
38	4507.5
39	5804.0
40	5804.0
41	6827.0
42	7850.0
43	8803.5
44	9757.0
45	12385.5
46	15014.0
47	15014.0
48	16913.5
49	18813.0
50	21296.0
51	23779.0
52	25568.5
53	27358.0
54	27358.0
55	25103.0
56	22848.0
57	22004.0
58	21160.0
59	19934.0
60	18708.0
61	18708.0
62	17344.5
63	15981.0
64	13576.0
65	11171.0
66	9869.0
67	8567.0
68	8567.0
69	7310.0
70	6053.0
71	5265.5
72	4478.0
73	3641.5
74	2805.0
75	2805.0
76	2305.5
77	1806.0
78	1427.0
79	1048.0
80	850.5
81	653.0
82	653.0
83	514.0
84	375.0
85	285.5
86	196.0
87	151.0
88	106.0
89	106.0
90	71.5
91	37.0
92	29.5
93	22.0
94	14.5
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	234056.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.34043251870793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.57139777354183	43.74163105660164
2	7.953004007543798	8.325273391557197
3	2.507548412443688	3.9373850540670614
4	1.3202095917291992	2.764013641858124
5	0.8519957960002603	2.2296914233387235
6	0.5791805078567045	1.8188734972574898
7	0.3993598075983267	1.463186554219908
8	0.34873150487895793	1.4602206238611648
9	0.2536019343285778	1.1946271437304852
>10	1.9554352380528397	19.979679672341703
>50	0.18524093384285334	6.575891740542626
>100	0.0733038989538564	6.202067097719064
>500	9.905932291061677E-4	0.3074591029048157
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	593	0.25335817069419286	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	412	0.17602625012817444	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	330	0.14099189937450868	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	301	0.12860170215674882	RNA PCR Primer, Index 47 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	299	0.1277472057969033	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	276	0.11792049765867998	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	253	0.10809378952045665	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	243	0.1038213077212291	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	242	0.10339405954130636	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	238	0.10168506682161534	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	238	0.10168506682161534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	4.2724817992275353E-4	0.0
20	0.0	0.0	0.0	4.2724817992275353E-4	0.0
21	0.0	0.0	0.0	0.0012817445397682605	0.0
22	0.0	0.0	0.0	0.0021362408996137678	0.0
23	0.0	0.0	0.0	0.0029907372594592746	0.0
24	0.0	0.0	0.0	0.0034179854393820283	0.0
25	0.0	0.0	0.0	0.004699729979150289	0.0
26	4.2724817992275353E-4	0.0	0.0	0.006408722698841303	0.0
27	4.2724817992275353E-4	0.0	0.0	0.0303346207745155	0.0
28	4.2724817992275353E-4	0.0	0.0	0.12347472399767577	0.0
29	4.2724817992275353E-4	0.0	0.0	0.27472057969033054	0.0
30	4.2724817992275353E-4	0.0	0.0	0.4699729979150289	0.0
31	4.2724817992275353E-4	0.0	0.0	0.936100762210753	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAACA	20	0.001838943	37.0	29
TCTATAC	20	0.001838943	37.0	3
GGTATCA	200	0.0	32.374996	1
AGTATTA	30	3.5908018E-4	30.833334	15
AAATCCG	25	0.005487515	29.599998	30
CTACCAC	25	0.005487515	29.599998	28
GGTCTAT	25	0.005487515	29.599998	1
TATACCC	25	0.005487515	29.599998	5
ATACGGC	55	6.2260006E-7	26.90909	29
ATCTAAT	35	8.848221E-4	26.42857	1
GCGGTAA	275	0.0	23.545456	23
AGATTTC	40	0.0019266201	23.125	21
CAGTATT	40	0.0019266201	23.125	14
TAATGTC	40	0.0019266201	23.125	4
TCGCCAT	65	2.6677171E-6	22.769234	13
TAATACG	260	0.0	22.769234	27
AATACGG	260	0.0	22.769234	28
ATGCCGT	75	3.7144855E-7	22.2	34
CCACGCT	50	2.6932033E-4	22.199999	20
GTAATAC	285	0.0	22.070175	26
>>END_MODULE